Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	217,138	A→G	Y546Y (TAT→TAC)	proS ←	prolyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	217,138	0	A	G	100.0%	177.2 / NA	50	Y546Y (TAT→TAC)	proS	prolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/21);  total (29/21)

TTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCAGCAGCACTTCGATACCTTGTGCA  >  NC_000913/217005‑217273                                                                                                                                      |                                                                                                                                        ttATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATAATCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATCTGTATTc                                                                                                                                    >  2:467592/1‑139 (MQ=255)      cAATCCATGTAAAAAAAGGGCCCTGCAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGatat                                                                                                                               <  1:328209/139‑1 (MQ=255)         tCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGGGACGATATTTGTATTCGATAtcgt                                                                                                                            >  2:137055/1‑139 (MQ=255)         tCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATAtcgt                                                                                                                            >  1:614815/1‑139 (MQ=255)             tGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGtt                                                                                                                        >  2:463558/1‑139 (MQ=255)             tGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGtt                                                                                                                        >  1:322742/1‑139 (MQ=255)              gTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTg                                                                                                                       >  1:608176/1‑139 (MQ=255)              gTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTg                                                                                                                       >  2:506007/1‑139 (MQ=255)                             tGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCg                                                                                                        <  2:322742/139‑1 (MQ=255)                               aaaTTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                      <  2:608176/139‑1 (MQ=255)                               aaaTTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                      <  1:463558/139‑1 (MQ=255)                                 aTTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCa                                                                                                    <  1:506007/139‑1 (MQ=255)                                    cAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGca                                                                                                 >  1:651454/1‑139 (MQ=255)                                       gACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACaa                                                                                              <  2:651454/139‑1 (MQ=255)                                           cTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAgt                                                                                          <  1:467592/139‑1 (MQ=255)                                            tttCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATAtcgtcg                                                                                                                          <  1:265653/106‑1 (MQ=255)                                            tttCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATAtcgtcg                                                                                                                          >  2:265653/1‑106 (MQ=255)                                                     tCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTc                                                                                                                     <  1:537679/102‑1 (MQ=255)                                                     tCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTc                                                                                                                     >  2:537679/1‑102 (MQ=255)                                                       aGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATAcc                                                                              >  2:708719/1‑139 (MQ=255)                                                          cTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGAt                                                                           <  1:708719/139‑1 (MQ=255)                                                                   gTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAgt                                                                                          <  2:395774/115‑1 (MQ=255)                                                                   gTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAgt                                                                                          >  1:395774/1‑115 (MQ=255)                                                                   gTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCat                                                                  >  2:303443/1‑139 (MQ=255)                                                                   gTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCat                                                                  >  2:87515/1‑139 (MQ=255)                                                                   gTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCat                                                                  >  1:575051/1‑139 (MQ=255)                                                                       cACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCa                                                              >  2:426853/1‑139 (MQ=255)                                                                             atatTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                      <  1:728474/93‑1 (MQ=255)                                                                             atatTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                      >  2:728474/1‑93 (MQ=255)                                                                                    acgaTGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGc                                                 >  1:444837/1‑139 (MQ=255)                                                                                        tGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                      >  1:714292/1‑82 (MQ=255)                                                                                        tGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGcc                                                                                                      <  2:714292/82‑1 (MQ=255)                                                                                        tGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCgg                                             >  2:617589/1‑139 (MQ=255)                                                                                        tGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCgg                                             >  1:222266/1‑139 (MQ=255)                                                                                                    taattaaCTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCg                                 <  1:87515/139‑1 (MQ=255)                                                                                                           cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCg                                                                                     <  1:544272/80‑1 (MQ=255)                                                                                                           cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCata                                                                 >  2:248031/1‑100 (MQ=255)                                                                                                           cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCata                                                                 <  1:248031/100‑1 (MQ=255)                                                                                                           cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATcc                          >  2:270473/1‑139 (MQ=255)                                                                                                           cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATcc                          >  1:128347/1‑139 (MQ=255)                                                                                                           cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATcc                          >  2:689428/1‑139 (MQ=255)                                                                                                           cTGTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTCGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCg                                                                                     >  2:544272/1‑80 (MQ=255)                                                                                                             gTTTCTCGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCcag                        >  1:573733/1‑139 (MQ=255)                                                                                                               ttCTCGCCGTTGCGACGATATTTGTATTCGATATCGACGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGGCATCcagca                      >  2:528679/1‑139 (MQ=255)                                                                                                                tctcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCcagcag                     <  2:614815/139‑1 (MQ=255)                                                                                                                tctcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCcagcag                     <  1:270473/139‑1 (MQ=255)                                                                                                                  tcGCCGTTGCGACGATATTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCAgcagca                   <  1:617589/139‑1 (MQ=255)                                                                                                                                atatTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCg                                                                                     <  1:753086/59‑1 (MQ=255)                                                                                                                                atatTTGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCg                                                                                     >  2:753086/1‑59 (MQ=255)                                                                                                                                   tttGTATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCAGCAGCACTTCGATACCTTGTGCa  <  1:137055/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTGTGCGGAATACCGATCAGTTCCATATCAGCAAACATCACGCCCGGACGCTCTTTGCGGTCATCCAGCAGCACTTCGATACCTTGTGCA  >  NC_000913/217005‑217273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑