Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 219,364 T→C P77P (CCA→CCG)  tsaA ← tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913219,3640TC97.1% 119.3 / ‑5.7 35P77P (CCA→CCG) tsaAtRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (17/17);  minor base A (0/1);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGT  >  NC_000913/219230‑219497
                                                                                                                                      |                                                                                                                                     
gcGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCGTTCAGCTCTACCAGCGACATGCCAATTGGGTGAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGAc                                                                                                                                   >  1:473359/2‑139 (MQ=255)
 cGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACg                                                                                                                                  >  1:656447/1‑139 (MQ=255)
          gAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCgg                                                                                                                         >  2:452363/1‑139 (MQ=255)
           aaTCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGa                                                                                                                        <  2:264921/139‑1 (MQ=255)
                 gCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCTGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAg                                                                                                                  <  2:552621/139‑1 (MQ=255)
                  cTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgc                                                                                                                 <  1:422669/139‑1 (MQ=255)
                  cTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCACCGAGGCGCGGCGGACGCACAGTCGGACGCCAgc                                                                                                                 >  1:493894/1‑139 (MQ=255)
                    gTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgccg                                                                                                               <  2:473359/139‑1 (MQ=255)
                            ggCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCAt                                                                                                       >  1:359284/1‑139 (MQ=255)
                               aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                                    >  1:667152/1‑139 (MQ=255)
                               aaaCAACCTCTTTCAGCTCTACCAGCGACATACCAACTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                                    >  2:712878/1‑139 (MQ=255)
                                aacaacCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGtt                                                                                                   >  1:651083/1‑139 (MQ=255)
                                 acaacCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGc                                                                                                                     >  2:156568/1‑120 (MQ=255)
                                 acaacCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGc                                                                                                                     <  1:156568/120‑1 (MQ=255)
                                 acaacCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGttt                                                                                                  >  2:579325/1‑139 (MQ=255)
                                        ctTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGaaa                                                                                           >  2:487322/1‑139 (MQ=255)
                                                    ccAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCAt                                                                                                       <  2:149741/115‑1 (MQ=255)
                                                    ccAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCAt                                                                                                       >  1:149741/1‑115 (MQ=255)
                                                         gACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATa                                                                          <  2:359284/139‑1 (MQ=255)
                                                                   ttGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                                <  1:19158/139‑1 (MQ=255)
                                                                   ttGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                                <  2:493894/139‑1 (MQ=255)
                                                                   ttGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                                <  1:569231/139‑1 (MQ=255)
                                                                   ttGGGTTACGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCGTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                                <  2:468846/139‑1 (MQ=255)
                                                                            ggCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaaa                                                                                   <  2:262172/111‑1 (MQ=255)
                                                                            ggCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaaa                                                                                   >  1:262172/1‑111 (MQ=255)
                                                                             gCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAgg                                                      >  1:174454/1‑139 (MQ=255)
                                                                             gCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAgg                                                      >  1:333611/1‑139 (MQ=255)
                                                                               ggAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGcc                                                    <  2:446654/139‑1 (MQ=255)
                                                                                gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCcg                                                   >  2:25025/1‑139 (MQ=255)
                                                                                               tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCtt                                                           >  1:283055/1‑116 (MQ=255)
                                                                                               tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCtt                                                           <  2:283055/116‑1 (MQ=255)
                                                                                                      aCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGt                             <  2:360073/139‑1 (MQ=255)
                                                                                                            aTTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAg                       <  1:579325/139‑1 (MQ=255)
                                                                                                              tCTGGCGTTACCGCCGAGGCGCGGAGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCa                     <  2:184655/139‑1 (MQ=255)
                                                                                                                                 cgcgGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGt  <  2:30842/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
CCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGT  >  NC_000913/219230‑219497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑