Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,379,348	C→T	*432* (TAG→TAA)	menF ←	isochorismate synthase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,379,348	0	C	T	100.0%	105.1 / NA	34	*432* (TAG→TAA)	menF	isochorismate synthase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/14);  total (20/14)

AGAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGA  >  NC_000913/2379216‑2379482                                                                                                                                     |                                                                                                                                       aGAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTcc                                                                                                                                  <  2:388574/139‑1 (MQ=255)   aaTGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                                >  2:440544/1‑139 (MQ=255)       aCCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                                <  1:178566/135‑1 (MQ=255)       aCCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                                >  2:178566/1‑135 (MQ=255)           ccgccCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                                >  1:329377/1‑131 (MQ=255)           ccgccCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCAt                                                                                                                                <  2:329377/131‑1 (MQ=255)            cgccCAGCGTCGGTTAAATGCGCTTACTAACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGtaataa                                                                                                                       <  1:461943/139‑1 (MQ=255)                       ggTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGcc                                                                                                            <  2:565801/139‑1 (MQ=255)                                                    tCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCAc                                                                               >  1:686095/1‑139 (MQ=255)                                                     ccGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCAct                                                                              >  2:365526/1‑139 (MQ=255)                                                            cAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                       <  2:633080/139‑1 (MQ=255)                                                                                 ggAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACg                                                                                                                 >  1:490584/1‑76 (MQ=255)                                                                                 ggAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACg                                                                                                                 <  2:490584/76‑1 (MQ=255)                                                                                    gCTACAAAAATAGATTATTGATATGATTCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGcc                                               <  2:282692/139‑1 (MQ=255)                                                                                     cTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGccc                                              >  2:574171/1‑139 (MQ=255)                                                                                      tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                       <  1:33624/113‑1 (MQ=255)                                                                                      tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTc                                                                       >  2:33624/1‑113 (MQ=255)                                                                                      tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcg                                             >  1:415000/1‑139 (MQ=255)                                                                                      tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcg                                             >  2:566516/1‑139 (MQ=255)                                                                                      tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcg                                             >  2:37570/1‑139 (MQ=255)                                                                                      tACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTAGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCcg                                             >  1:460550/1‑139 (MQ=255)                                                                                         aaaaaTAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                          <  1:574171/139‑1 (MQ=255)                                                                                         aaaaaTAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                          <  1:440544/139‑1 (MQ=255)                                                                                         aaaaaTAGATTATTGATATGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCgcgc                                          <  1:365526/139‑1 (MQ=255)                                                                                                          atGAATCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACg                         >  1:109734/1‑139 (MQ=255)                                                                                                               tCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                                <  2:378972/95‑1 (MQ=255)                                                                                                               tCGGTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                                >  1:378972/1‑95 (MQ=255)                                                                                                                  gTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGcc                 >  2:66108/1‑139 (MQ=255)                                                                                                                  gTAATGATGCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGcc                 >  2:679399/1‑139 (MQ=255)                                                                                                                         tgCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAAtt          >  1:765212/1‑139 (MQ=255)                                                                                                                          gCGACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAAttt         >  1:120324/1‑139 (MQ=255)                                                                                                                            gACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                                >  2:244265/1‑82 (MQ=255)                                                                                                                            gACTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCg                                                                <  1:244265/82‑1 (MQ=255)                                                                                                                             aCTCATTATTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTg      >  2:199964/1‑139 (MQ=255)                                                                                                                                 attattattCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGa  >  2:642377/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                       AGAATGACCGCCGCCCAGCGTCGGTTAAATGCGCTTACTGACATGAGTTTGTCCGGTATCAATATTGCGGCTAAGTATAAGGAGCTACAAAAATAGATTATTGATATGAATCGGTAATGATGCGACTCATTACTATTCCATTTGTAATAAAGTACGCAGCCCTGCCGCTTTGTTGTCGATTTCCTGCCACTCTTGCTCGGGGTCGGAACCACGGACAATGCCCGCGCCAGCATATAATCGCACGACATTGCCGCTAATTTTTGCTGA  >  NC_000913/2379216‑2379482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑