| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,414,699 | T→C | intergenic (+27/‑48) | ackA → / → pta | acetate kinase A and propionate kinase 2/phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,414,699 | 0 | T | C | 96.5% | 91.5 / ‑4.4 | 29 | intergenic (+27/‑48) | ackA/pta | acetate kinase A and propionate kinase 2/phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (13/15); total (13/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
CGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCC > NC_000913/2414562‑2414807 | cGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTg < 2:581130/139‑1 (MQ=255) gTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGgcgc < 2:135431/139‑1 (MQ=255) tGGTTGCATCCACAAAACAGGACCACGTACTACGGTGGTTATCCCAACCAACATAGCACGGTTTATCGGGAAGGACGCGAGCCCCGTGTCTGGCTCATTTCAAACCGCAAGCTCAGCCCGCGGCGCTGTTTTGTAAccc < 2:734614/139‑1 (MQ=255) acaaAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATcggc < 2:735977/139‑1 (MQ=255) gTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa < 1:420170/139‑1 (MQ=255) cccGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAaga > 1:725373/1‑139 (MQ=255) gtTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTc < 2:27071/139‑1 (MQ=255) aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg > 1:579483/1‑139 (MQ=255) aTCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCg < 2:725373/139‑1 (MQ=255) ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAag < 1:288903/114‑1 (MQ=255) ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAag > 2:288903/1‑114 (MQ=255) ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtattatt > 1:13842/1‑139 (MQ=255) ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGtattatt < 2:292057/139‑1 (MQ=255) acGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATg > 2:684980/1‑139 (MQ=255) tttATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCATACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATaaa > 1:473839/2‑108 (MQ=255) gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa > 1:30362/1‑98 (MQ=255) gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGaa < 2:30362/98‑1 (MQ=255) gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATaaa > 1:89215/1‑108 (MQ=255) gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATaaa < 2:89215/108‑1 (MQ=255) gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATaaa < 2:473839/108‑1 (MQ=255) aGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCg < 2:257994/139‑1 (MQ=255) aGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCg > 1:434740/1‑139 (MQ=255) cgccTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGAc < 1:564544/139‑1 (MQ=255) gccTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGAcc < 2:591492/139‑1 (MQ=255) ccTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCa > 2:711613/1‑139 (MQ=255) gCCTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAg > 1:768143/1‑139 (MQ=255) ccTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGc > 2:99701/1‑139 (MQ=255) cTGATTTCACACCGCCAGCTCAGCTGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGcc < 1:464085/139‑1 (MQ=255) ctcagctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGAc > 1:120572/1‑110 (MQ=255) ctcagctGGCGGCGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGAc < 2:120572/110‑1 (MQ=255) | CGTTTCGGCAAATCTGGTTTCATCAACAAAGAAGGTACCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCC > NC_000913/2414562‑2414807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |