Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,539,230	G→A	G118G (GGC→GGT)	cysM ←	cysteine synthase B (O‑acetylserine sulfhydrolase B)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,539,230	0	G	A	100.0%	71.8 / NA	24	G118G (GGC→GGT)	cysM	cysteine synthase B (O‑acetylserine sulfhydrolase B)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/11);  total (13/11)

GTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATG  >  NC_000913/2539123‑2539365                                                                                                            |                                                                                                                                        gtggtggtgTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCg                                                                                                          <  2:755190/139‑1 (MQ=255) gtggtggtgTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCg                                                                                                          <  1:726879/139‑1 (MQ=255)               cgcATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGc                                                                                            >  1:125854/1‑139 (MQ=255)                           aTCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACgg                                                                                >  2:711828/1‑139 (MQ=255)                                       gAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTc                                                                    <  2:589541/139‑1 (MQ=255)                                          cTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAAt                                                                                                                  <  1:731408/90‑1 (MQ=255)                                          cTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAAt                                                                                                                  >  2:731408/1‑90 (MQ=255)                                             aTCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGa                                                                                                                         <  1:535839/80‑1 (MQ=255)                                             aTCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGa                                                                                                                         >  2:535839/1‑80 (MQ=255)                                             aTCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACg                                                                                 >  2:374867/1‑120 (MQ=255)                                             aTCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACg                                                                                 <  1:374867/120‑1 (MQ=255)                                             aTCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCAtgttg                                                              >  1:614656/1‑139 (MQ=255)                                                                         gCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTTATGTTGTCGGGCATCAGCAATTTCATGCGATAGc                                  >  1:267994/1‑139 (MQ=255)                                                                             tctcCAGCGCCAGATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGAACCATAAGCAGGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCttt                              <  2:717719/139‑1 (MQ=255)                                                                                         gATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCA‑GTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCgg                      >  1:272828/1‑134 (MQ=255)                                                                                         gATCGCGCGCACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCA‑GTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCgg                      <  2:272828/134‑1 (MQ=255)                                                                                              cgcgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTg             >  2:534246/1‑139 (MQ=255)                                                                                              cgcgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTg             >  1:184036/1‑139 (MQ=255)                                                                                              cgcgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTg             >  1:17972/1‑139 (MQ=255)                                                                                              cgcgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTg             >  1:144985/1‑139 (MQ=255)                                                                                               gcgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGc            <  2:646201/139‑1 (MQ=255)                                                                                                cgcACCTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGcc           <  2:125854/139‑1 (MQ=255)                                                                                                     cTTCCATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAgcgc      >  1:651136/1‑139 (MQ=255)                                                                                                         cATACCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATg  <  1:534246/139‑1 (MQ=255)                                                                                                            |                                                                                                                                        GTGGTGGTGTAATGCGCATAAGGGTTATCGGGATTATTGAACTGATCGAGCAGCTTTCCTTCGCCACGATTCGCCATCTCCAGCGCCAGATCGCGCGCACCTTCCATGCCCTGCTCTTTGGTGACAAGAATCAGTTCCGCACCATAAGCACGCATCGCCGCACGGCGTTCCTGGCTCATGTTGTCGGGCATCAGCAATTTCATGCGATAGCCTTTCAGCGCGGCAATCATTGCCAGCGCAATG  >  NC_000913/2539123‑2539365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑