| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,662,226 | A→T | intergenic (‑95/+357) | iscR ← / ← trmJ | isc operon transcriptional repressor, suf operon transcriptional activator, oxidative stress‑ and iron starvation‑inducible, autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,662,226 | 0 | A | T | 98.0% | 157.1 / NA | 50 | intergenic (‑95/+357) | iscR/trmJ | isc operon transcriptional repressor, suf operon transcriptional activator, oxidative stress‑ and iron starvation‑inducible, autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (26/23); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTAAATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTG > NC_000913/2662103‑2662356 | gCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTAttt > 1:269438/1‑139 (MQ=255) ccTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTg > 2:220729/1‑139 (MQ=255) cAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCggg > 1:276821/1‑139 (MQ=255) gTCTTCCTTCACCTCAAACTCGCCCCTGCCCGGGGTTTCATATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGTCCTTCCCGAGTATATTGGTCAACTATTTACTTTACTGATTGCGTCGGGCATTTAAcc > 2:638802/1‑139 (MQ=255) tACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCa > 1:708153/1‑139 (MQ=255) ccTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCAt > 1:442558/1‑139 (MQ=255) cTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCAtt > 1:724185/1‑139 (MQ=255) aCTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACt > 2:274779/1‑89 (MQ=255) aCTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACt < 1:274779/89‑1 (MQ=255) cGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGc > 2:667571/1‑127 (MQ=255) cGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGc < 1:667571/127‑1 (MQ=255) ccccTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCgg < 2:559324/107‑1 (MQ=255) ccccTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCgg > 1:559324/1‑107 (MQ=255) ccccTGCCCGGGGTCTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGt > 1:241669/1‑139 (MQ=255) gCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGt > 1:228868/1‑139 (MQ=255) ggTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTAc < 2:276821/139‑1 (MQ=255) ggTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTAc < 1:220729/139‑1 (MQ=255) tttATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTc < 2:620577/64‑1 (MQ=255) tttATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTc > 1:620577/1‑64 (MQ=255) tttATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCtgtt > 1:521461/1‑139 (MQ=255) tAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGAt > 1:311652/1‑139 (MQ=255) aaaGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGAAAATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGTTgc > 1:22614/1‑139 (MQ=255) ggTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGaa > 1:17048/1‑139 (MQ=255) tGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTg > 1:101347/1‑55 (MQ=255) tGCATAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTg < 2:101347/55‑1 (MQ=255) aTAGCAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGCATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCatat < 2:442558/139‑1 (MQ=255) cAGGGTCAAGTCTGACATTCCCGAGTATATTGGTc > 1:692154/1‑35 (MQ=255) cAGGGTCAAGTCTGACATTCCCGAGTATATTGGTc < 2:692154/35‑1 (MQ=255) cAGGGTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGc > 1:676804/1‑139 (MQ=255) ggTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCg > 1:676826/1‑58 (MQ=255) ggTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCg < 2:676826/58‑1 (MQ=255) ggTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTAt > 1:629204/1‑88 (MQ=255) ggTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTAt < 2:629204/88‑1 (MQ=255) ggTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGt < 2:415428/91‑1 (MQ=255) ggTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGt > 1:415428/1‑91 (MQ=255) ggTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGCCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTg < 1:712768/99‑1 (MQ=255) ggTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGCCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTg > 2:712768/1‑99 (MQ=255) gTCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTg < 2:269438/139‑1 (MQ=255) tCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCa > 2:45793/1‑71 (MQ=255) tCAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCa < 1:45793/71‑1 (MQ=255) cAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTggg < 2:521461/139‑1 (MQ=255) cAAGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTggg < 2:676804/139‑1 (MQ=255) aGTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGt > 2:95524/1‑139 (MQ=255) gTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGtt < 2:241669/139‑1 (MQ=255) gTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGtt < 1:739008/139‑1 (MQ=255) gTCTGACATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGtt < 2:724185/139‑1 (MQ=255) aCATTCCCGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTa < 2:228868/139‑1 (MQ=255) cccGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCgg < 2:248485/43‑1 (MQ=255) cccGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCgg > 1:248485/1‑43 (MQ=255) cccGAGTATATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTg < 2:708153/139‑1 (MQ=255) | GCATAGCGCCCTTTAGATGTCAGTCTCATGTCTTACTTCACCTCAAACTCGCCCCTGCCCGGGGTTTTTTATTGTAAAAGTGGGGGTATTGCATAGCAGGGTCAAGTCTGACATTCCCGAGTAAATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTG > NC_000913/2662103‑2662356 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |