Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	251,932	A→G	R345R (AGA→AGG)	dinB →	DNA polymerase IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	251,932	0	A	G	100.0%	195.5 / NA	55	R345R (AGA→AGG)	dinB	DNA polymerase IV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/37);  total (18/37)

AGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCA  >  NC_000913/251799‑252041                                                                                                                                      |                                                                                                              aGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCaa                                                                                                            >  2:349114/1‑137 (MQ=255) aGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCaa                                                                                                            <  1:349114/137‑1 (MQ=255)     cGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATCGAAAGGCAACTGGTg                                                                                                      >  1:161163/1‑139 (MQ=255)      gTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGc                                                                                                     <  2:530136/139‑1 (MQ=255)      gTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGc                                                                                                     <  1:672845/139‑1 (MQ=255)          ggCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTggg                                                                                                 >  2:583455/1‑139 (MQ=255)           gCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGa                                                                                                <  1:141032/139‑1 (MQ=255)           gCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGa                                                                                                <  1:341574/139‑1 (MQ=255)             cgcgGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGAtt                                                                                              <  1:583455/139‑1 (MQ=255)             cgcgGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGAtt                                                                                              <  1:400047/139‑1 (MQ=255)                ggCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTatg                                                                                           <  1:244100/139‑1 (MQ=255)                ggCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTatg                                                                                           <  2:33377/139‑1 (MQ=255)                ggCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTatg                                                                                           <  2:594112/139‑1 (MQ=255)                  cTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATCGAAAGGCAACTGGTGCTGGGATTatgat                                                                                         <  2:161163/139‑1 (MQ=255)                    gAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGt                                                                                       <  1:433411/139‑1 (MQ=255)                     aaTAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGta                                                                                      <  1:486417/139‑1 (MQ=255)                       tAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGtata                                                                                    <  1:327690/139‑1 (MQ=255)                        aaaGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATAc                                                                                   <  1:448535/139‑1 (MQ=255)                           gCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACtat                                                                                <  2:46960/139‑1 (MQ=255)                              gATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACtattat                                                                             <  1:221607/139‑1 (MQ=255)                                       gCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCt                                                                    <  1:139683/139‑1 (MQ=255)                                          aCCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGgt                                                                 <  2:613787/139‑1 (MQ=255)                                          aCCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGgt                                                                 <  2:75717/139‑1 (MQ=255)                                           ccGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGc                                                                                                     >  2:687307/1‑102 (MQ=255)                                           ccGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGc                                                                                                     <  1:687307/102‑1 (MQ=255)                                              cgcgTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGc                                                             >  1:437858/1‑139 (MQ=255)                                                  tAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACAGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCatta                                                         >  2:636106/1‑139 (MQ=255)                                                      aCCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATtattat                                                     <  1:636106/139‑1 (MQ=255)                                                        cTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGa                                                   <  1:621599/139‑1 (MQ=255)                                                        cTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGa                                                   <  2:414348/139‑1 (MQ=255)                                                           ggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGAtt                                                                                              >  1:557065/1‑93 (MQ=255)                                                           ggATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGAtt                                                                                              <  2:557065/93‑1 (MQ=255)                                                              tGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTAt                                             <  1:99876/139‑1 (MQ=255)                                                                  cgccgcGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACtat                                                                                <  1:303532/100‑1 (MQ=255)                                                                  cgccgcGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACtat                                                                                >  2:303532/1‑100 (MQ=255)                                                                             gcgcGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGa                              >  1:551417/1‑139 (MQ=255)                                                                                       cGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTatgat                                                                                         >  2:208242/1‑70 (MQ=255)                                                                                       cGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTatgat                                                                                         <  1:208242/70‑1 (MQ=255)                                                                                             gtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGtata                                                                                    >  1:717427/1‑69 (MQ=255)                                                                                             gtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGtata                                                                                    <  2:717427/69‑1 (MQ=255)                                                                                             gtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGgt                                                                 >  2:268170/1‑88 (MQ=255)                                                                                             gtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGgt                                                                 <  1:268170/88‑1 (MQ=255)                                                                                             gtggGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAAt              >  1:444191/1‑139 (MQ=255)                                                                                               ggggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCg            <  1:460900/139‑1 (MQ=255)                                                                                                 ggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATtattat                                                     >  2:75738/1‑96 (MQ=255)                                                                                                 ggCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATtattat                                                     <  1:75738/96‑1 (MQ=255)                                                                                                      cATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGAtt                                                                                              >  1:420068/1‑50 (MQ=255)                                                                                                      cATGTGACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGAtt                                                                                              <  2:420068/50‑1 (MQ=255)                                                                                                         gtgACGTTGCTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTCTGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCa  <  2:551417/139‑1 (MQ=255)                                                                                                                  cTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGtata                                                                                    >  2:581577/1‑48 (MQ=255)                                                                                                                  cTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGtata                                                                                    <  1:581577/48‑1 (MQ=255)                                                                                                                  cTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGgtgt                                                               >  1:351939/1‑69 (MQ=255)                                                                                                                  cTTGACCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGgtgt                                                               <  2:351939/69‑1 (MQ=255)                                                                                                                      aCCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGgt                                                                 >  1:619421/1‑63 (MQ=255)                                                                                                                      aCCCGCAAATGGAAAGGCAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGgt                                                                 <  2:619421/63‑1 (MQ=255)                                                                                                                                      |                                                                                                              AGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCATTATTATGAGGGTATCACTGTATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCA  >  NC_000913/251799‑252041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑