Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	253,084	A→G	T126A (ACA→GCA)	yafP →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	253,084	0	A	G	100.0%	115.0 / NA	33	T126A (ACA→GCA)	yafP	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/14);  total (19/14)

ACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGACAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTGCGCGTTTTCTGATTCCAC  >  NC_000913/252959‑253218                                                                                                                              |                                                                                                                                       aCCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcagca                                                                                                                           >  2:41560/1‑139 (MQ=255)        aTACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGtt                                                                                                                    <  1:236155/139‑1 (MQ=255)        aTACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGtt                                                                                                                    <  2:744171/139‑1 (MQ=255)        aTACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGtt                                                                                                                    <  2:307330/139‑1 (MQ=255)                 ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCgg                                                                                                           >  1:371632/1‑139 (MQ=255)                 ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCgg                                                                                                           >  2:697987/1‑139 (MQ=255)                 ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCgg                                                                                                           >  1:484289/1‑139 (MQ=255)                 ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCgg                                                                                                           >  2:634481/1‑139 (MQ=255)                     ggggTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAg                                                                                                       <  2:484289/139‑1 (MQ=255)                           gCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGt                                                                                                 >  2:416810/1‑139 (MQ=255)                            ccAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGtt                                                                                                >  2:142072/1‑139 (MQ=255)                                                 tGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAATGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAg                                                                                                       >  1:597871/1‑111 (MQ=255)                                                 tGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAg                                                                                                       <  2:597871/111‑1 (MQ=255)                                                 tGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGata                                                                           >  2:700711/1‑139 (MQ=255)                                                       aGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAAcc                                                                     >  2:244617/1‑139 (MQ=255)                                                       aGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAAcc                                                                     >  1:169276/1‑139 (MQ=255)                                                                   aaCTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATg                                                                                <  2:87724/116‑1 (MQ=255)                                                                   aaCTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATg                                                                                >  1:87724/1‑116 (MQ=255)                                                                          ggTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCtt                                                  <  2:415496/139‑1 (MQ=255)                                                                          ggTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGAGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCtt                                                  >  1:22268/1‑139 (MQ=255)                                                                            tGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGc                                                <  1:627665/139‑1 (MQ=255)                                                                                cGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                                     >  1:68681/1‑82 (MQ=255)                                                                                cGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                                     <  2:68681/82‑1 (MQ=255)                                                                                cGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATg                                            >  2:52530/1‑139 (MQ=255)                                                                                         aaCCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcg                                   <  1:41560/139‑1 (MQ=255)                                                                                          aCCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcgc                                  <  1:416810/139‑1 (MQ=255)                                                                                           ccGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcgcc                                 <  1:52530/139‑1 (MQ=255)                                                                                           ccGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcgcc                                 >  2:664870/1‑139 (MQ=255)                                                                                             gCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcgcccg                               >  2:521450/1‑139 (MQ=255)                                                                                              cAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAAttttt                                                                                     <  2:403063/84‑1 (MQ=255)                                                                                              cAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAAttttt                                                                                     >  1:403063/1‑84 (MQ=255)                                                                                                                         ttCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTGCGCGTTTTCTGATTCca   >  1:557346/1‑139 (MQ=255)                                                                                                                          tCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTGCGCGTTTTCTGATTCcac  <  1:697987/139‑1 (MQ=255)                                                                                                                              |                                                                                                                                       ACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGACAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATGTGCGCGTTTTCTGATTCCAC  >  NC_000913/252959‑253218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑