| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,787,434 | G→A | pseudogene (1038/2253 nt) | ygaQ → | uncharacterized protein, putative enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,787,434 | 0 | G | A | 97.0% | 208.7 / ‑1.0 | 66 | pseudogene (1038/2253 nt) | ygaQ | uncharacterized protein, putative enzyme |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (30/34); minor base T (1/1); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
CGATATCATTCCTGATTATACCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAGCAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACATCTCTAAGGATGAATGGGAA > NC_000913/2787302‑2787569 | cGATATCATTCCTGATTATACCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGt < 1:751038/139‑1 (MQ=255) cTGATTATACCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGAt > 1:719629/1‑139 (MQ=255) gATTATACCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTc > 1:144672/1‑139 (MQ=255) tataCCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCcat < 1:262685/139‑1 (MQ=255) aCCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCcattca > 2:318544/1‑139 (MQ=255) ttATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATcc < 1:318544/139‑1 (MQ=255) aTGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGAtt > 1:404646/1‑120 (MQ=255) aTGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGAtt < 2:404646/120‑1 (MQ=255) aTGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAg > 1:17853/1‑139 (MQ=255) tGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAGGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGt < 1:263510/139‑1 (MQ=255) tGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACCAGGTCAGGTTTTGATTCca > 1:510036/1‑106 (MQ=255) tGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca < 2:510036/106‑1 (MQ=255) ggTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca > 1:688101/1‑105 (MQ=255) ggTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca < 2:688101/105‑1 (MQ=255) aaaaTGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAat < 1:108815/139‑1 (MQ=255) gACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAatccatc > 1:196920/1‑139 (MQ=255) aCCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAatccatcc < 2:120393/139‑1 (MQ=255) ccTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCcatt < 1:772297/96‑1 (MQ=255) ccTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCcatt > 2:772297/1‑96 (MQ=255) gATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCagaag < 2:549559/139‑1 (MQ=255) gATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCagaag > 2:699196/1‑139 (MQ=255) tCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGAtt > 1:164682/1‑82 (MQ=255) tCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGAtt < 2:164682/82‑1 (MQ=255) tCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca < 2:245972/85‑1 (MQ=255) tCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca > 1:245972/1‑85 (MQ=255) ctgctgATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACAtct > 1:53951/1‑139 (MQ=255) ctgctgATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACAtct < 1:664342/139‑1 (MQ=255) gctgATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGGTATTAACAAGGTCAGGTTTTGAGTCCAATCATTAAAGATCCAGTAACAAAAGAACGTAAACGAATCCATCCATAAGATATACATCTCa < 2:719629/139‑1 (MQ=255) gctgATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGACGATATACATCTCa < 2:17853/139‑1 (MQ=255) gctgATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCa < 2:94446/139‑1 (MQ=255) atatACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGc > 2:432203/1‑139 (MQ=255) aGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCcattcat < 1:450783/72‑1 (MQ=255) aGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCcactcat > 2:450783/1‑72 (MQ=255) aGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGAc > 2:240265/1‑139 (MQ=255) aGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGAc > 2:618430/1‑139 (MQ=255) atatAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGaa < 2:386386/128‑1 (MQ=255) atatAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGaa > 1:386386/1‑128 (MQ=255) taGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGt > 2:764653/1‑131 (MQ=255) taGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGt < 1:764653/131‑1 (MQ=255) aGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc < 2:144672/139‑1 (MQ=255) aGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc < 1:618430/139‑1 (MQ=255) aGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc < 2:335273/139‑1 (MQ=255) aGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc < 1:699196/139‑1 (MQ=255) gAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAg > 1:381805/1‑124 (MQ=255) gAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAg < 2:381805/124‑1 (MQ=255) ggtggtATCCCTGGCTATTATCAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACAtct > 2:304540/1‑139 (MQ=255) gtggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGAAAAAGACTTCGAAGCAAGTAAAGATAACAtct > 1:308634/1‑138 (MQ=255) gtggtATCCCTGGCTATTAACAAAGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACAtct < 2:308634/138‑1 (MQ=255) tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAatccatcc > 2:61619/1‑84 (MQ=255) tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAatccatcc < 1:61619/84‑1 (MQ=255) tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTaaa < 2:365522/127‑1 (MQ=255) tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTaaa > 1:365522/1‑127 (MQ=255) tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACATctct > 2:157123/1‑139 (MQ=255) ggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc > 2:21512/1‑132 (MQ=255) ggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc < 1:21512/132‑1 (MQ=255) gtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAg > 2:213831/1‑117 (MQ=255) gtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAg < 1:213831/117‑1 (MQ=255) gtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACAt > 2:180518/1‑133 (MQ=255) gtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACAt < 1:180518/133‑1 (MQ=255) gtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACATCTCTaa > 2:619406/1‑139 (MQ=255) ccTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACATCTCTAAGGATg < 1:129600/139‑1 (MQ=255) cTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAAc > 2:105546/1‑60 (MQ=255) cTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAAc < 1:105546/60‑1 (MQ=255) tATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAg > 2:606524/1‑106 (MQ=255) tATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAg < 1:606524/106‑1 (MQ=255) ttATCAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACATCTCTAAGGATGAATGGGaa < 1:304540/139‑1 (MQ=255) | CGATATCATTCCTGATTATACCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAGCAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACATCTCTAAGGATGAATGGGAA > NC_000913/2787302‑2787569 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |