Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,851,873	G→A	*291* (TAG→TAA)  G4S (GGC→AGC)	hypB → hypC →	GTP hydrolase involved in nickel liganding into hydrogenases hydrogenase maturation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,851,873	0	G	A	100.0%	45.7 / NA	16	*291* (TAG→TAA)  G4S (GGC→AGC)	hypB hypC	GTP hydrolase involved in nickel liganding into hydrogenases hydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/5);  total (11/5)

TGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTG  >  NC_000913/2851744‑2852003                                                                                                                                  |                                                                                                                                   tGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCg                                                                                                                           >  2:522005/1‑139 (MQ=255)                 aTCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCAt                                                                                                           <  1:233616/139‑1 (MQ=255)                              gcgAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGTGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAg                                                                                              >  2:699368/1‑139 (MQ=255)                               cgAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAgg                                                                                             >  1:486362/1‑139 (MQ=255)                                             aaaTTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGTGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTc                                                                               >  1:607976/1‑139 (MQ=255)                                                                  ccGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTc                                                          >  2:473044/1‑139 (MQ=255)                                                                    gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAgcg                                                                 <  1:64359/130‑1 (MQ=255)                                                                    gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAgcg                                                                 >  2:64359/1‑130 (MQ=255)                                                                     ccaccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGAt                                                       >  1:277450/1‑139 (MQ=255)                                                                      caccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGAtt                                                      <  2:615485/139‑1 (MQ=255)                                                                            cggcgAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGTGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGt                                                <  2:607976/139‑1 (MQ=255)                                                                                            cAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGa                                >  2:767282/1‑139 (MQ=255)                                                                                                    gAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACg                        >  1:272589/1‑139 (MQ=255)                                                                                                        tggctggAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCa                    >  2:673869/1‑139 (MQ=255)                                                                                                                gaCACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCcgcgcg            <  1:699368/139‑1 (MQ=255)                                                                                                                          tgtgCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTg  >  1:241335/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                   TGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTG  >  NC_000913/2851744‑2852003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑