Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	262,563	G→A	R354H (CGT→CAT)	proA →	gamma‑glutamylphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	262,563	0	G	A	100.0%	114.0 / NA	36	R354H (CGT→CAT)	proA	gamma‑glutamylphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/11);  total (25/11)

GATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATG  >  NC_000913/262433‑262693                                                                                                                                   |                                                                                                                                   gATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTa                                                                                                                            <  2:355873/139‑1 (MQ=255)   tGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAAc                                                                                                                          >  1:486113/1‑139 (MQ=255)                ttAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGt                                                                                                             >  2:426699/1‑139 (MQ=255)                 tAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTc                                                                                                            >  1:386956/1‑139 (MQ=255)                 tAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTc                                                                                                            >  2:325581/1‑139 (MQ=255)                  aGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGa                                                                                                                  <  1:263113/132‑1 (MQ=255)                  aGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGa                                                                                                                  >  2:263113/1‑132 (MQ=255)                   gATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCg                                                                                                          <  1:628693/139‑1 (MQ=255)                       tGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGt                                                                                                      >  2:324512/1‑139 (MQ=255)                       tGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGt                                                                                                      >  1:601580/1‑139 (MQ=255)                        gAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGtt                                                                                                     <  1:325581/139‑1 (MQ=255)                              cAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGtt                                                                                               >  2:731412/1‑139 (MQ=255)                                         gCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTAc                                                                                    >  1:478053/1‑139 (MQ=255)                                                      tGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGAc                                                                       >  1:240555/1‑139 (MQ=255)                                                       gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTg                                                                                                                    >  1:649547/1‑93 (MQ=255)                                                       gCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTg                                                                                                                    <  2:649547/93‑1 (MQ=255)                                                                     tCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGt                                                        <  2:386956/139‑1 (MQ=255)                                                                         gAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTgg                                                    >  1:648287/1‑139 (MQ=255)                                                                         gAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTgg                                                    >  2:452736/1‑139 (MQ=255)                                                                               ggCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCgg                                              >  2:728423/1‑139 (MQ=255)                                                                                                  cGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGcacaca                           >  1:770803/1‑139 (MQ=255)                                                                                                  cGATCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGcacaca                           >  1:300711/1‑139 (MQ=255)                                                                                                     tCCTGACCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACaaaa                        >  2:119473/1‑139 (MQ=255)                                                                                                          aCCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTcc                   <  2:300711/139‑1 (MQ=255)                                                                                                          aCCCGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTcc                   >  2:388940/1‑139 (MQ=255)                                                                                                           cccGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCa                  <  1:324512/139‑1 (MQ=255)                                                                                                           cccGCGATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCa                  <  2:478053/139‑1 (MQ=255)                                                                                                               cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcg              >  1:569019/1‑139 (MQ=255)                                                                                                               cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcg              <  1:119473/139‑1 (MQ=255)                                                                                                               cgATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcg              >  1:151235/1‑139 (MQ=255)                                                                                                               cgATATGCGCAACGACCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTCGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcg              >  1:468718/1‑139 (MQ=255)                                                                                                                gATATGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCAcgcgc             <  1:237699/139‑1 (MQ=255)                                                                                                                    tGCGCAACGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTgg         >  1:711930/1‑139 (MQ=255)                                                                                                                          aCGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAAt   >  1:579018/1‑139 (MQ=255)                                                                                                                          aCGCCCAGCATTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAAt   >  1:436323/1‑139 (MQ=255)                                                                                                                           cGCCCAGCATTTTGTTAACGAAGTGGACTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATg  >  2:460575/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                   GATGAGTTTCTGTCATTAGATTTGAACGTCAAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACACTCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGATTCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTGGGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATG  >  NC_000913/262433‑262693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑