Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,913,699	C→T	*434* (TAG→TAA)	rlmD ←	23S rRNA m(5)U1939 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,913,699	0	C	T	100.0%	185.8 / NA	57	*434* (TAG→TAA)	rlmD	23S rRNA m(5)U1939 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/31);  total (26/31)

TAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTA  >  NC_000913/2913573‑2913834                                                                                                                               |                                                                                                                                        tAATGCCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcgc                                                                                                                             <  1:508433/139‑1 (MQ=255) tAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcgc                                                                                                                             >  2:722957/1‑139 (MQ=255) tAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcgc                                                                                                                             <  2:288226/139‑1 (MQ=255) tAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAAcgcgc                                                                                                                             <  2:507941/139‑1 (MQ=255)          gACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGt                                                                                                                    >  1:553795/1‑139 (MQ=255)          gACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGt                                                                                                                    >  2:766329/1‑139 (MQ=255)               gCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                               <  2:67141/139‑1 (MQ=255)                     cATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTc                                                                                                         <  2:552201/139‑1 (MQ=255)                               gATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTccc                                                                                               <  1:760556/139‑1 (MQ=255)                               gATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTccc                                                                                               <  2:757035/139‑1 (MQ=255)                                aTCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                                                              <  1:199240/139‑1 (MQ=255)                                aTCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCg                                                                                              <  1:428097/139‑1 (MQ=255)                                 tCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgt                                                                                             <  1:722957/139‑1 (MQ=255)                                      ttCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtgtg                                                                                        <  1:160670/139‑1 (MQ=255)                                         aCCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGAGAGAAAAGTACCATCAATTCCAGATGTCCCGTCTGTGGGa                                                                                     <  1:470872/139‑1 (MQ=255)                                                    tGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGc                                                                          <  1:766329/139‑1 (MQ=255)                                                        atGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCg                                                                      >  2:411226/1‑139 (MQ=255)                                                           tgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCa                                                                   >  1:729786/1‑139 (MQ=255)                                                           tgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCa                                                                   >  1:642644/1‑139 (MQ=255)                                                           tgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCa                                                                   >  1:497513/1‑139 (MQ=255)                                                           tgCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTAAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCa                                                                   >  1:437776/1‑139 (MQ=255)                                                             cACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt                                                                 >  1:538449/1‑139 (MQ=255)                                                             cACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt                                                                 >  1:737359/1‑139 (MQ=255)                                                                ttcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt                                                                 >  2:49445/1‑136 (MQ=255)                                                                ttcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGt                                                                 <  1:49445/136‑1 (MQ=255)                                                                ttcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgc                                                              >  1:414050/1‑139 (MQ=255)                                                                 tcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                             <  2:497513/139‑1 (MQ=255)                                                                 tcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                             <  2:435094/139‑1 (MQ=255)                                                                 tcttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTcgcg                                                             <  1:240006/139‑1 (MQ=255)                                                                  cttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                               >  1:313519/1‑88 (MQ=255)                                                                  cttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCAt                                                                                                               <  2:313519/88‑1 (MQ=255)                                                                  cttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCgcgc                                                            <  2:414050/139‑1 (MQ=255)                                                                  cttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTAAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCgcgc                                                            <  2:437776/139‑1 (MQ=255)                                                                   ttACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCa                                                           <  2:195151/139‑1 (MQ=255)                                                                      acGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATg                                                        >  1:356066/2‑139 (MQ=255)                                                                          aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg                                                                                            <  1:758821/99‑1 (MQ=255)                                                                          aaCCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCgtg                                                                                            >  2:758821/1‑99 (MQ=255)                                                                            ccATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTatat                                                  >  2:763170/1‑139 (MQ=255)                                                                             cATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATc                                                 <  2:553795/139‑1 (MQ=255)                                                                             cATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATc                                                 <  2:729786/139‑1 (MQ=255)                                                                             cATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATc                                                 <  1:651775/139‑1 (MQ=255)                                                                                cGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGa                                                                                                     >  2:718089/1‑84 (MQ=255)                                                                                cGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGa                                                                                                     <  1:718089/84‑1 (MQ=255)                                                                                    ctctCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCttt                                          <  2:15398/139‑1 (MQ=255)                                                                                      ctcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATc                                                 <  2:195351/130‑1 (MQ=255)                                                                                      ctcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATc                                                 >  1:195351/1‑130 (MQ=255)                                                                                       tcCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTtaa                                       >  1:182005/1‑139 (MQ=255)                                                                                            tAGGGACCAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACg                                  >  1:765374/1‑139 (MQ=255)                                                                                                   cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTccc                                                                                               >  2:347517/1‑71 (MQ=255)                                                                                                   cAGACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTccc                                                                                               <  1:347517/71‑1 (MQ=255)                                                                                                     gACCTGCCGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTAt                         >  2:50242/1‑139 (MQ=255)                                                                                                      aCCTGCCGAAATCGGCAAATCGCAATTATTTAAcg                                                                                                                                >  2:756758/1‑35 (MQ=255)                                                                                                      aCCTGCCGAAATCGGCAAATCGCAATTATTTAAcg                                                                                                                                <  1:756758/35‑1 (MQ=255)                                                                                                           ccGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAg                   >  2:697114/1‑139 (MQ=255)                                                                                                           ccGAAATCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAg                   >  1:654837/1‑139 (MQ=255)                                                                                                                aTCGGCAAATCGCAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGc              >  2:190845/1‑139 (MQ=255)                                                                                                                            cAATTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTa  <  1:411226/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                        TAATACCCAGACTTGCGATCCATTTTTCCGGATCAAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTGTGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTA  >  NC_000913/2913573‑2913834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑