| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,914,462 | A→G | L180P (CTG→CCG) | rlmD ← | 23S rRNA m(5)U1939 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,914,462 | 0 | A | G | 100.0% | 70.8 / NA | 21 | L180P (CTG→CCG) | rlmD | 23S rRNA m(5)U1939 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/10); total (11/10) | |||||||||||
CCAGTTTTTCGCGATCTGCCGAACTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCAGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCA > NC_000913/2914343‑2914597 | ccAGTTTTTCGCGATCTGCCGAACTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTggg < 1:36342/139‑1 (MQ=255) cgcgATCTGCCGAACTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGc > 1:704389/1‑139 (MQ=255) ccGAACTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGAc > 1:447168/1‑105 (MQ=255) aCTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGgc < 2:538639/139‑1 (MQ=255) cggtATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATggg > 1:5307/1‑139 (MQ=255) ggtATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATgggg < 2:48275/139‑1 (MQ=255) cAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTg > 2:731968/1‑139 (MQ=255) aaaTCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGAc < 1:281498/139‑1 (MQ=255) atcaGCGTGCCGCTGGTTGTCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGa < 1:625192/139‑1 (MQ=255) cGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCa < 2:543062/115‑1 (MQ=255) cGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCa > 1:543062/1‑115 (MQ=255) tACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGgcagc < 1:618382/70‑1 (MQ=255) tACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGgcagc > 2:618382/1‑70 (MQ=255) ttCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAAc < 2:563068/139‑1 (MQ=255) ttCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAAc > 1:206980/1‑139 (MQ=255) aGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTc > 2:129467/1‑139 (MQ=255) aGCTTGTAAGCTGCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCACTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTc > 1:466334/1‑139 (MQ=255) gCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACaa < 1:247695/77‑1 (MQ=255) gCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACaa > 2:247695/1‑77 (MQ=255) gCCCGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACGATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTc < 2:747355/139‑1 (MQ=255) cccGGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCa > 1:769391/1‑139 (MQ=255) | CCAGTTTTTCGCGATCTGCCGAACTTAGCGGTGCGGTATGGCGCAAAATCATCAGCGTGCCGCTGGTTGCCTGTACCAGTTCAACATGACCAAGATGGCGCATAGCTTGTAAGCTGCCCAGACATGCCCTGACTTTGGGCAGCAATGCTTCAAGTTGGGGCGCTAAAATGGGGCATTGTTTGACGTCGACAATGTCACTGGAGCCCGCTTTGCGAAACCCCATCTGAAGTTGCTGTGTTTTCGGTAAGTAGTTCA > NC_000913/2914343‑2914597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |