Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 264,882 C→T A109T (GCT→ACT)  yafX ← CP4‑6 prophage, uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913264,8820CT100.0% 132.2 / NA 41A109T (GCT→ACT) yafXCP4‑6 prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)

AGTCGATAATACGCAGAATGGCGTGAGCCTCTGGATGTTGCATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGCAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCGGGGGACATAAATGCGCCGCCATTGCTGAGGGTGTAGAACGACCA  >  NC_000913/264750‑265011
                                                                                                                                    |                                                                                                                                 
aGTCGATAATACGCAGAATGGCGTGAGCCTCTGGATGTTGCATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATAcc                                                                                                                             >  1:464914/1‑139 (MQ=255)
  tCGATAATACGCAGAATGGCGTGAGCCTCTGGATGTTGCATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACctg                                                                                                                           >  1:42987/1‑139 (MQ=255)
   cGATAATACGCAGAATGGCGTGAGCCTCTGGATGTTGCATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACctgc                                                                                                                          <  1:211464/139‑1 (MQ=255)
          aCGCAGAATGGCGTGAGCCTCTGGATGTTGCATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCt                                                                                                                   >  1:148802/1‑139 (MQ=255)
            gCAGAATGGCGTGAGCCTCTGGATGTTGCATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGc                                                                                                                 >  2:659540/1‑139 (MQ=255)
                   ggCGTGAGCCTCTGGATGTTGCATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCAtt                                                                                                          >  1:436430/1‑139 (MQ=255)
                          gCCTCTGGATGTTGCATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCAt                                                                                                   <  2:148802/139‑1 (MQ=255)
                                    gTTGCATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGtt                                                                                         <  1:622806/139‑1 (MQ=255)
                                    gTTGCATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGtt                                                                                         >  2:622806/1‑139 (MQ=255)
                                      tGCATGGCATACTCCCGCAGGCGGAAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCa                                                                                       >  1:144955/1‑139 (MQ=255)
                                        cATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAgg                                                                                     >  2:561882/1‑139 (MQ=255)
                                        cATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAgg                                                                                     <  1:245357/139‑1 (MQ=255)
                                        cATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAgg                                                                                     <  2:42987/139‑1 (MQ=255)
                                           ggCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCa                                                                                                                >  2:453804/1‑109 (MQ=255)
                                           ggCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCa                                                                                                                <  1:453804/109‑1 (MQ=255)
                                             cATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGtt                                                                                >  1:312983/1‑139 (MQ=255)
                                             cATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGtt                                                                                >  2:657816/1‑139 (MQ=255)
                                                       cAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTg                                                                                                        >  2:649458/1‑105 (MQ=255)
                                                       cAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTg                                                                                                        <  1:649458/105‑1 (MQ=255)
                                                       cAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGc                                                                      <  2:436430/139‑1 (MQ=255)
                                                              tAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGtct                                                               <  1:210151/139‑1 (MQ=255)
                                                              tAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGtct                                                               <  2:312983/139‑1 (MQ=255)
                                                                    tgtgCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGt                                                         >  1:617994/1‑139 (MQ=255)
                                                                        cGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTc                                                     >  1:478371/1‑139 (MQ=255)
                                                                              tCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTc                                               >  2:545108/1‑139 (MQ=255)
                                                                               cgcgTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCg                                              <  1:561882/139‑1 (MQ=255)
                                                                                               cGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCGGGGGACATAAATgcgc                              <  2:478371/139‑1 (MQ=255)
                                                                                               cGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCGGGGGACATAAATgcgc                              <  1:412242/139‑1 (MQ=255)
                                                                                                ggcaggcaTGGTGGCTATACGCAATCAGGCAGACAGTAATACTTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCGGGGGACATAAATGcgcc                             >  1:745064/1‑139 (MQ=255)
                                                                                                ggcaggcaTGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCGGGGGACATAAATGcgcc                             >  2:40046/1‑139 (MQ=255)
                                                                                                 gcaggcaTGGTGGCTATACGCAATCAGGCAGACAGTAATACctgct                                                                                                                         <  2:26402/46‑1 (MQ=255)
                                                                                                 gcaggcaTGGTGGCTATACGCAATCAGGCAGACAGTAATACctgct                                                                                                                         >  1:26402/1‑46 (MQ=255)
                                                                                                  caggcaTGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCa                                                                                                    >  2:407129/1‑66 (MQ=255)
                                                                                                  caggcaTGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCa                                                                                                    <  1:407129/66‑1 (MQ=255)
                                                                                                  caggcaTGGTGGCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCGGGGGACATAAATGcgccgc                           <  1:657816/139‑1 (MQ=255)
                                                                                                         ggtggCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCGGGGGACATAAATGCGCCGCCATTGCt                    <  1:124034/139‑1 (MQ=255)
                                                                                                         ggtggCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCGGGGGACATAAATGCGCCGCCATTGCt                    <  1:168423/139‑1 (MQ=255)
                                                                                                           tggCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTa                                                                                              <  1:742246/63‑1 (MQ=255)
                                                                                                           tggCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTa                                                                                              >  2:742246/1‑63 (MQ=255)
                                                                                                           tggCTATACGCAATCAGGCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCGGGGGACATAAATGCGCCGCCATTGCTGa                  <  1:240215/139‑1 (MQ=255)
                                                                                                                           ggCAGACAGTAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCGGGGGACATAAATGCGCCGCCATTGCTGAGGGTGTAGAACGACCa  >  2:123542/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                 
AGTCGATAATACGCAGAATGGCGTGAGCCTCTGGATGTTGCATGGCATACTCCCGCAGGCGGTAATAGTGTGCGGTCATCGCGTCACATTCTGTACGGCAGGCATGGTGGCTATACGCAATCAGGCAGACAGCAATACCTGCTGCTTCTGCACTCATTTGGGCATCGTTACCGTTCAGGCAGTTAAACAGACGCCATGTCTCATCGTTGTCAGGCTCGGGGGACATAAATGCGCCGCCATTGCTGAGGGTGTAGAACGACCA  >  NC_000913/264750‑265011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑