Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,075,233 A→G I317T (ATA→ACA)  yggF ← fructose 1,6 bisphosphatase isozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,075,2330AG100.0% 143.3 / NA 40I317T (ATA→ACA) yggFfructose 1,6 bisphosphatase isozyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/19);  total (21/19)

CCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTC  >  NC_000913/3075097‑3075367
                                                                                                                                        |                                                                                                                                      
ccccTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtgtt                                                                                                                                      <  2:28154/139‑1 (MQ=255)
ccccTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCtgtt                                                                                                                                      <  2:344815/139‑1 (MQ=255)
                gTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGt                                                                                                                      <  2:374487/139‑1 (MQ=255)
                   tGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACGCGTTTGGTCcg                                                                                                                   >  1:504654/1‑139 (MQ=255)
                   tGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCcg                                                                                                                   <  1:390278/139‑1 (MQ=255)
                     cgcAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCcgcg                                                                                                                 <  2:144275/139‑1 (MQ=255)
                        aGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              >  1:197528/1‑139 (MQ=255)
                        aGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTACCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGcgccg                                                                                                              <  2:425841/139‑1 (MQ=255)
                          ttttCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCgccgcc                                                                                                            <  1:86693/139‑1 (MQ=255)
                          ttttCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCgccgcc                                                                                                            <  1:294048/139‑1 (MQ=255)
                                       aaataaatTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTc                                                                                               <  2:248318/139‑1 (MQ=255)
                                                  ccTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCAc                                                                                    >  2:10184/1‑139 (MQ=255)
                                                     gtgCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCAcccc                                                                                 >  1:520043/1‑139 (MQ=255)
                                                          tCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCg                                                                            <  1:10184/139‑1 (MQ=255)
                                                           ccAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGa                                                                                                          <  1:266370/108‑1 (MQ=255)
                                                           ccAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGa                                                                                                          >  2:266370/1‑108 (MQ=255)
                                                                  cTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTa                                                                                                    >  2:240316/1‑107 (MQ=255)
                                                                  cTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTa                                                                                                    <  1:240316/107‑1 (MQ=255)
                                                                  cTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGt                                                                    >  2:512129/1‑139 (MQ=255)
                                                                  cTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGt                                                                    >  2:539994/1‑139 (MQ=255)
                                                                            tcatcTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGt                                                          >  2:504516/1‑139 (MQ=255)
                                                                                    tGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGc                                                  >  2:622211/1‑139 (MQ=255)
                                                                                     gTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCt                                                 >  2:728934/1‑139 (MQ=255)
                                                                                       cAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCa                                               >  1:245297/1‑139 (MQ=255)
                                                                                          cGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCg                                            <  2:245297/139‑1 (MQ=255)
                                                                                               ccATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGt                                       <  1:154993/139‑1 (MQ=255)
                                                                                                         tCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTa                             >  1:720240/1‑139 (MQ=255)
                                                                                                         tCCGATAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTa                             >  1:343887/1‑139 (MQ=255)
                                                                                                             aTAGATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAgcgc                         >  1:86598/1‑139 (MQ=255)
                                                                                                                gATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTaa                      <  2:720240/139‑1 (MQ=255)
                                                                                                                gATCACCATCAATGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTaa                      <  1:60428/139‑1 (MQ=255)
                                                                                                                           aTGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAg                                                                                                      >  2:454029/1‑48 (MQ=255)
                                                                                                                           aTGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAg                                                                                                      <  1:454029/48‑1 (MQ=255)
                                                                                                                           aTGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCa                                                                                     <  2:728891/65‑1 (MQ=255)
                                                                                                                           aTGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCa                                                                                     >  1:728891/1‑65 (MQ=255)
                                                                                                                            tGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGt          <  2:655992/139‑1 (MQ=255)
                                                                                                                            tGCAGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGt          >  1:655992/1‑139 (MQ=255)
                                                                                                                               aGGGAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTcc       <  1:539994/139‑1 (MQ=255)
                                                                                                                                 ggAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAaga                   <  1:52198/125‑1 (MQ=255)
                                                                                                                                 ggAGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAaga                   >  2:52198/1‑125 (MQ=255)
                                                                                                                                   aGTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCt   >  1:424804/1‑139 (MQ=255)
                                                                                                                                    gTCTGTTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTc  >  1:297638/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CCCCTGGAGCAAAATGGTATGCGCAGTTTTCATAAATGAAAATAAATTGTCCTGTGCATCCAGTTGCTCAAGCACATCATCTTCTGTCAGCGTCGCCATTGGTCTTCCGATAGATCACCATCAATGCAGGGAGTCTATTATATTACACGTTTGGTCCGCGCCGCCGATCAGTAATGTCTGCGTCCGCACCCCATTCGCCGTCTGTTGGATACCGTTCACCAGCTCACCGCCCGTCACGCCCGTAGCGCTAAAGAGGATATCGTTCCCCCTC  >  NC_000913/3075097‑3075367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: