Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,257,675	G→A	G112G (GGC→GGT)	yhaO ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,257,675	0	G	A	100.0%	109.7 / NA	35	G112G (GGC→GGT)	yhaO	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/20);  total (15/20)

AAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGAT  >  NC_000913/3257540‑3257798                                                                                                                                        |                                                                                                                            aaaGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATAccc                                                                                                                          <  2:76011/139‑1 (MQ=255) aaaGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGCTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATAccc                                                                                                                          <  2:442520/139‑1 (MQ=255)       aCTGTCTGACAGCAACCCTTCCGTCACGACGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCCCACCTAACAGGATACCCCAGttt                                                                                                                    >  1:477014/1‑139 (MQ=255)                cAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTaaa                                                                                                           <  2:367356/139‑1 (MQ=255)                     aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAAcc                                                                                                      >  1:493589/1‑139 (MQ=255)                               agcGCCGAAGGTATGCAAGTAAGAAGCACTATCGTTGGTGATGGCCGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACg                                                                                            <  2:480511/137‑1 (MQ=255)                                            tGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTCTGACCTAAATAACGGATAATGACGATCGGGTaaacct                                                                               >  1:480511/1‑135 (MQ=255)                                                         cACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGa                                                                  >  1:69100/1‑139 (MQ=255)                                                          aCTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCCCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAt                                                                 <  2:477014/139‑1 (MQ=255)                                                           cTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAtt                                                                >  1:288194/1‑139 (MQ=255)                                                           cTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAtt                                                                >  1:525096/1‑139 (MQ=255)                                                           cTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAtt                                                                >  1:66833/1‑139 (MQ=255)                                                                 ttGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCa                                                          <  1:292272/139‑1 (MQ=255)                                                                 ttGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCa                                                          <  2:424526/139‑1 (MQ=255)                                                                                       cGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTg                                    <  2:525096/139‑1 (MQ=255)                                                                                             tCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCgg                                                                                       <  2:404736/82‑1 (MQ=255)                                                                                             tCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCgg                                                                                       >  1:404736/1‑82 (MQ=255)                                                                                             tCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                     <  1:455088/84‑1 (MQ=255)                                                                                             tCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                     >  2:455088/1‑84 (MQ=255)                                                                                               cAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGAt                            <  2:156457/139‑1 (MQ=255)                                                                                                aaaTCACCTGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATACACAATCGCt                                     <  1:517726/129‑1 (MQ=255)                                                                                                aaaTCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCt                                     >  2:517726/1‑129 (MQ=255)                                                                                                       cAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGcc                    >  1:657872/1‑139 (MQ=255)                                                                                                        aGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCg                   >  1:174079/1‑139 (MQ=255)                                                                                                           aTTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGt                <  2:174079/139‑1 (MQ=255)                                                                                                             tACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTaa              <  2:66833/139‑1 (MQ=255)                                                                                                               cGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAAcc            <  2:493589/139‑1 (MQ=255)                                                                                                                       agCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCaa          <  2:178062/133‑1 (MQ=255)                                                                                                                       agCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCaa          >  1:178062/1‑133 (MQ=255)                                                                                                                         cgcACCTAACTGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCt                                                                                          <  1:23633/51‑1 (MQ=255)                                                                                                                         cgcACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCt                                                                                          >  2:23633/1‑51 (MQ=255)                                                                                                                         cgcACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGAt  <  2:470118/139‑1 (MQ=255)                                                                                                                         cgcACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGAt  <  2:69100/139‑1 (MQ=255)                                                                                                                               tAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                       >  2:189352/1‑80 (MQ=255)                                                                                                                               tAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                       <  1:189352/80‑1 (MQ=255)                                                                                                                                        |                                                                                                                            AAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGAT  >  NC_000913/3257540‑3257798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑