Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,683,630	C→T	*663* (TAG→TAA)	yhjK ←	cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,683,630	0	C	T	100.0%	86.8 / NA	28	*663* (TAG→TAA)	yhjK	cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/12);  total (16/12)

CCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCA  >  NC_000913/3683494‑3683744                                                                                                                                         |                                                                                                                   ccTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTa                                                                                                                  >  1:155414/1‑139 (MQ=255) ccTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTa                                                                                                                  >  1:331249/1‑139 (MQ=255)                              caTCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGa                                                                                     >  1:669427/1‑139 (MQ=255)                                  acaGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACACACCAGCACTTTTTCAAGTTTAGTAATCAGTTTGGGTTAGTTACTTTTCTTCCAGATAACTCTCTTCGAAGATTTc                                                                                 >  2:385243/1‑139 (MQ=255)                                   caGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGAtt                                                                                   <  2:541586/136‑1 (MQ=255)                                   caGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGAtt                                                                                   >  1:541586/1‑136 (MQ=255)                                                ggATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGc                                                                   >  1:727242/1‑139 (MQ=255)                                                ggATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGc                                                                   <  2:669427/139‑1 (MQ=255)                                                 gATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCg                                                                  >  1:34695/1‑139 (MQ=255)                                                 gATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCg                                                                  >  2:232526/1‑139 (MQ=255)                                                  aTCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGa                                                                 >  2:29809/1‑139 (MQ=255)                                                           gAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                        <  1:372998/139‑1 (MQ=255)                                                           gAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                        >  1:154171/1‑139 (MQ=255)                                                                aaaaaGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGcc                                                   <  2:122013/139‑1 (MQ=255)                                                                 aaaaGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGccc                                                  <  1:543480/139‑1 (MQ=255)                                                                     ggTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTggg                                              <  2:285230/139‑1 (MQ=255)                                                                                ttGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCaa                                                             <  2:410515/113‑1 (MQ=255)                                                                                ttGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTAGAAGATTTCAATAGGGAGTGGGCGAGCaa                                                             >  1:410515/1‑113 (MQ=255)                                                                                               caAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTgccagc                    <  2:155414/139‑1 (MQ=255)                                                                                                  aCCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                        <  1:657838/100‑1 (MQ=255)                                                                                                  aCCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAgg                                                        >  2:657838/1‑100 (MQ=255)                                                                                                  aCCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCTGCTTTTGccagccag                 >  1:165171/1‑139 (MQ=255)                                                                                                  aCCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGccagccag                 >  1:641647/1‑139 (MQ=255)                                                                                                     aGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCC‑‑GGGCAATACCAACGCCCGCTTTTGCCAGCCAGTcgcg            <  2:727242/139‑1 (MQ=255)                                                                                                            tttAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTg                                                >  2:332359/1‑98 (MQ=255)                                                                                                            tttAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTg                                                <  1:332359/98‑1 (MQ=255)                                                                                                            tttAAAGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTtgtg       >  2:579136/1‑139 (MQ=255)                                                                                                                 aGTTTTGTAATCAGTTTGGGGTAGTTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCa  <  1:579136/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                   CCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAATCAGTTTGGGGTAGCTACTTTTCTTCCAGGTAACTCTCTTCGAAGATTTCAATAGGGAGTGGGCGAGCAAAAAGGAAGCCCTGGGCAATACCAACGCCCGCTTTTGCCAGCCAGTCGCGTTGTGCTTCA  >  NC_000913/3683494‑3683744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑