Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,694,840	G→A	H133Y (CAT→TAT)	bcsA ←	cellulose synthase, catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,694,840	0	G	A	100.0%	107.0 / NA	34	H133Y (CAT→TAT)	bcsA	cellulose synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/19);  total (15/19)

TGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATGCTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCA  >  NC_000913/3694710‑3694968                                                                                                                                   |                                                                                                                                 tGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGttt                                                                                                                          >  1:770246/1‑139 (MQ=255)  gcgcCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGtttt                                                                                                                         >  1:715232/1‑139 (MQ=255)  gcgcCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGtttt                                                                                                                         >  1:569134/1‑139 (MQ=255)     ccAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATg                                                                                                                      >  1:443327/1‑139 (MQ=255)      cAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATgg                                                                                                                     <  1:436046/139‑1 (MQ=255)          gggTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCTCTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                 <  1:268874/139‑1 (MQ=255)          gggTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                 <  1:243657/139‑1 (MQ=255)           ggTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAAc                                                                                                                >  2:702489/1‑139 (MQ=255)                   ggCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAg                                                                                                        <  2:243507/139‑1 (MQ=255)                      tGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCa                                                                                                     >  2:349859/1‑139 (MQ=255)                           aaCGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCg                                                                                                <  1:676418/139‑1 (MQ=255)                              gCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTc                                                                                             <  2:770246/139‑1 (MQ=255)                                aGATTAACGCCAGAATCCGCGAGAAGGTGACGATGATACCGAGGATCGCCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCAGCCAGATACTTTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCAt                                                                                           <  2:270221/139‑1 (MQ=255)                                aGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCAt                                                                                           <  2:569134/139‑1 (MQ=255)                                aGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCAt                                                                                           <  2:552503/139‑1 (MQ=255)                                aGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCAt                                                                                           <  2:354526/139‑1 (MQ=255)                                  aTTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATcc                                                                                         >  2:244417/1‑139 (MQ=255)                                        gCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGAt                                                                                   <  2:443327/139‑1 (MQ=255)                                                 aGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTc                                                                          <  2:578875/139‑1 (MQ=255)                                                                    tACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGAcc                                                       <  1:349859/139‑1 (MQ=255)                                                                       cGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGa                                                    >  2:533699/1‑139 (MQ=255)                                                                           gATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAAt                                                >  2:460861/1‑139 (MQ=255)                                                                                aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTc                                                                          <  1:352733/108‑1 (MQ=255)                                                                                aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTc                                                                          >  2:352733/1‑108 (MQ=255)                                                                                aCCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCg                                           >  2:576488/1‑139 (MQ=255)                                                                                     cgcgCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTgc                                      >  1:212816/1‑139 (MQ=255)                                                                                                         aCCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCa                                                                                                         >  1:679657/1‑52 (MQ=255)                                                                                                         aCCGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCa                                                                                                         <  2:679657/52‑1 (MQ=255)                                                                                                           cGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                 >  2:232114/1‑42 (MQ=255)                                                                                                           cGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTaa                                                                                                                 <  1:232114/42‑1 (MQ=255)                                                                                                           cGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTgcg                <  1:533699/139‑1 (MQ=255)                                                                                                           cGAGCTCTTTTTTCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTgcg                <  1:244417/139‑1 (MQ=255)                                                                                                                    ttttCTCATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCAcc       <  1:460861/139‑1 (MQ=255)                                                                                                                         tcATCCAGATACTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCa  >  1:200509/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                 TGCGCCAGCGGGTTAAACGGCTGAGTAACGCAGATTAACGCCAGAATCAGCGAGAAGGTGACGATGATACCGAGGATCAACCGCCGCGCCCCGGCACTCAAATGACCGAGCTCTTTTTTCTCATCCAGATGCTGTGTTTTATGGCTAACGCGCTCAGGCAGCTCGTTCATCCATTGATGGTAACGTCCACGAATATTTTGCAGACCTGAAAATGCCCGCCTGCGCGGTTTCGGCGTTTCTTTGCGCGATGCACCGATCA  >  NC_000913/3694710‑3694968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑