Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,767,179	G→A	*281* (TAG→TAA)	yibA →	putative immunity protein for polymorphic toxin RhsA, HEAT‑domain protein, lethality reduction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,767,179	0	G	A	100.0%	172.6 / NA	53	*281* (TAG→TAA)	yibA	putative immunity protein for polymorphic toxin RhsA, HEAT‑domain protein, lethality reduction protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/22);  total (31/22)

TATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTG  >  NC_000913/3767048‑3767313                                                                                                                                    |                                                                                                                                       tATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGt                                                                                                                                 <  1:282576/139‑1 (MQ=255) tATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGt                                                                                                                                 <  1:643994/139‑1 (MQ=255)         tatCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATg                                                                                                                         >  2:440914/1‑139 (MQ=255)           tCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGca                                                                                                                       >  1:294241/1‑139 (MQ=255)           tCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGca                                                                                                                       <  1:96273/139‑1 (MQ=255)           tCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGca                                                                                                                       <  1:689481/139‑1 (MQ=255)              ttGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAg                                                                                                                    >  1:597652/1‑139 (MQ=255)                     tGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGa                                                                                                                                   >  2:57089/1‑117 (MQ=255)                     tGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGa                                                                                                                                   <  1:57089/117‑1 (MQ=255)                       cGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGAtt                                                                                                           >  1:507002/1‑139 (MQ=255)                         ggTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTcc                                                                                                         >  2:749103/1‑139 (MQ=255)                         ggTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTcc                                                                                                         >  1:681836/1‑139 (MQ=255)                           tGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCccg                                                                                                       >  2:8509/1‑139 (MQ=255)                              aTTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCccgccg                                                                                                    >  1:62793/1‑139 (MQ=255)                               ttAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCgg                                                                                                   <  2:47854/139‑1 (MQ=255)                               ttAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCgg                                                                                                   <  2:62793/139‑1 (MQ=255)                                tAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGc                                                                                                  <  1:1816/139‑1 (MQ=255)                                 aGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                                 <  2:507002/139‑1 (MQ=255)                                 aGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCa                                                                                                 <  2:294241/139‑1 (MQ=255)                                  ggTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCAc                                                                                                <  2:397667/139‑1 (MQ=255)                                            cGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATg                                                                                      >  2:630469/1‑139 (MQ=255)                                             gCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGc                                                                                     >  2:107719/1‑139 (MQ=255)                                                  ttCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGAc                                                                                <  1:82484/139‑1 (MQ=255)                                                       gTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCt                                                                           >  2:361227/1‑139 (MQ=255)                                                                  tATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGaaa                                                                >  2:417168/1‑139 (MQ=255)                                                                    tgttgtACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATc                                                              >  2:478196/1‑139 (MQ=255)                                                                       tgtACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTg                                                           >  1:573395/1‑139 (MQ=255)                                                                        gtACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTgg                                                          <  1:630469/139‑1 (MQ=255)                                                                         tACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGAAAAGCTGAAGCGTTCATAACGTGAGTTGCCTAAGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGc                                                         >  1:473555/1‑139 (MQ=255)                                                                          aCAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCt                                                        <  1:440914/139‑1 (MQ=255)                                                                           cAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTg                                                       >  1:360051/1‑139 (MQ=255)                                                                              gTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGAcc                                                    >  2:687525/1‑139 (MQ=255)                                                                              gTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGAcc                                                    >  1:513985/1‑139 (MQ=255)                                                                                ttGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCgg                                                  <  2:360051/139‑1 (MQ=255)                                                                                   atgaCAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCggggg                                               >  2:78337/1‑137 (MQ=255)                                                                                   atgaCAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                               >  2:23482/1‑139 (MQ=255)                                                                                   atgaCAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGgtg                                               >  1:10383/1‑139 (MQ=255)                                                                                                  taACTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAgccgc                                >  2:100074/1‑139 (MQ=255)                                                                                                    aCTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGccgccg                              <  2:597652/139‑1 (MQ=255)                                                                                                    aCTTCCGCTATTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGccgccg                              <  1:6434/139‑1 (MQ=255)                                                                                                             aTTGATAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCtggctggc                     <  1:671232/139‑1 (MQ=255)                                                                                                                  tAAGCTGAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGt                <  1:8509/139‑1 (MQ=255)                                                                                                                        gAAGCGTTCATAACGTGAGTTGCCTATGCACGGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGcc          >  2:304971/1‑139 (MQ=255)                                                                                                                        gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGa                                                      <  2:424289/95‑1 (MQ=255)                                                                                                                        gAAGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGa                                                      >  1:424289/1‑95 (MQ=255)                                                                                                                         aaGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt         >  2:466062/1‑139 (MQ=255)                                                                                                                         aaGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCt         >  2:699276/1‑139 (MQ=255)                                                                                                                          aGCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTg        >  1:294889/1‑139 (MQ=255)                                                                                                                           gCGTTCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGa       >  2:482283/1‑139 (MQ=255)                                                                                                                              ttCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGcc          <  2:2910/133‑1 (MQ=255)                                                                                                                              ttCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGcc          >  1:2910/1‑133 (MQ=255)                                                                                                                               tCATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGt   >  1:461016/1‑139 (MQ=255)                                                                                                                                cATAACGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTg  <  1:100074/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       TATGATGATATCATTGAAGCTGCGGGTGAATTAGGTGATAAAACGCTACTTCCTGTTTTAGATACTATGTTGTACAAGTTTGATGACAATGAAATTATAACTTCCGCTATTGATAAGCTGAAGCGTTCATAGCGTGAGTTGCCTATGCACAGTGGGGGATTCCCGCCGGCACGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTG  >  NC_000913/3767048‑3767313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑