Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,047	C→T	A316V (GCT→GTT)  *345* (TAG→TAA)	yibQ → waaH ←	putative polysaccharide deacetylase LPS(HepIII)‑glucuronic acid glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,047	0	C	T	100.0%	145.2 / NA	45	A316V (GCT→GTT)  *345* (TAG→TAA)	yibQ waaH	putative polysaccharide deacetylase LPS(HepIII)‑glucuronic acid glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/20);  total (25/20)

CCGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTT  >  NC_000913/3788914‑3789181                                                                                                                                      |                                                                                                                                       ccGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAgcg                                                                                                                                   <  2:622742/139‑1 (MQ=255)                       aTCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTa                                                                                                             >  2:370931/1‑139 (MQ=255)                       aTCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTa                                                                                                             >  1:388078/1‑139 (MQ=255)                            ttcttTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCg                                                                                                        >  1:688584/1‑139 (MQ=255)                                        ttAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcg                                                                                            >  1:242665/1‑139 (MQ=255)                                        ttAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcg                                                                                            >  1:421642/1‑139 (MQ=255)                                        ttAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcg                                                                                            >  1:466024/1‑139 (MQ=255)                                         tAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcgc                                                                                           >  1:744949/1‑139 (MQ=255)                                           aGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGt                                                                                         >  2:358515/1‑139 (MQ=255)                                           aGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGACGCTACGCGTAATGCGATGGTGCGACCGCGt                                                                                         >  2:628989/1‑139 (MQ=255)                                                  gCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTcg                                                                                  <  2:242665/139‑1 (MQ=255)                                                     tCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCgcgc                                                                               >  2:296420/1‑139 (MQ=255)                                                     tCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTATGTCgcgc                                                                               >  2:468241/1‑139 (MQ=255)                                                           agagCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGc                                                                         <  2:484309/139‑1 (MQ=255)                                                                   cTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCg                                                                 >  1:759846/1‑139 (MQ=255)                                                                   cTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGAGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAgcg                                                                                                                                   <  2:299239/73‑1 (MQ=255)                                                                   cTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGAGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAgcg                                                                                                                                   >  1:299239/1‑73 (MQ=255)                                                                       tCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGaaa                                                             >  2:591337/1‑139 (MQ=255)                                                                            tACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACca                                                        >  1:181290/1‑139 (MQ=255)                                                                                tCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcg                                                                                                                 <  1:60653/78‑1 (MQ=255)                                                                                tCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcg                                                                                                                 >  2:60653/1‑78 (MQ=255)                                                                                tCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTACCATAAGCGGAAAGACCACAGt                                                    >  2:322620/1‑139 (MQ=255)                                                                                         aGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTgg                                           >  2:51917/1‑139 (MQ=255)                                                                                           tGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTa                                         >  1:642970/1‑139 (MQ=255)                                                                                             gCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAAc                                       >  1:537120/1‑139 (MQ=255)                                                                                              cAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTATCGCCTGGTAAcc                                      <  2:466024/139‑1 (MQ=255)                                                                                                aGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGa                                    >  2:656475/1‑139 (MQ=255)                                                                                                  gCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGAcc                                  >  1:387125/1‑139 (MQ=255)                                                                                                    tGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCtt                                <  2:388078/139‑1 (MQ=255)                                                                                                     ggggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGaaa                                                             <  1:690842/109‑1 (MQ=255)                                                                                                     ggggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGaaa                                                             >  2:690842/1‑109 (MQ=255)                                                                                                     ggggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTc                               >  1:759973/1‑139 (MQ=255)                                                                                                       ggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCAc                             >  1:240225/1‑139 (MQ=255)                                                                                                        gCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACg                            <  1:370931/139‑1 (MQ=255)                                                                                                          aaaCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCt                          <  1:51917/139‑1 (MQ=255)                                                                                                                           gAAGTTTAACGTTAGCGCAAATTAAAGACGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCa         <  1:628989/139‑1 (MQ=255)                                                                                                                             aGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCGCATTGGTAATCAgg       <  1:358515/139‑1 (MQ=255)                                                                                                                             aGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAgg       <  2:163622/139‑1 (MQ=255)                                                                                                                             aGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAgg       <  1:656475/139‑1 (MQ=255)                                                                                                                             aGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAgg       <  2:759973/139‑1 (MQ=255)                                                                                                                             aGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAgg       <  2:90331/139‑1 (MQ=255)                                                                                                                               tttAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGgcg     <  1:60869/139‑1 (MQ=255)                                                                                                                               tttAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGgcg     <  1:296420/139‑1 (MQ=255)                                                                                                                                ttAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGgcgc    <  1:591337/139‑1 (MQ=255)                                                                                                                                  aaCGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCtt  <  2:181290/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       CCGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTT  >  NC_000913/3788914‑3789181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑