Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,870,441	G→A	*355* (TAG→TAA)  G430G (GGC→GGT)	cbrA → dgoT ←	colicin M resistance protein, FAD‑binding protein, putative oxidoreductase D‑galactonate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,870,441	0	G	A	100.0%	192.2 / NA	59	*355* (TAG→TAA)  G430G (GGC→GGT)	cbrA dgoT	colicin M resistance protein, FAD‑binding protein, putative oxidoreductase D‑galactonate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/31);  total (28/31)

CTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATG  >  NC_000913/3870305‑3870576                                                                                                                                         |                                                                                                                                        cTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAAcc                                                                                                                                       >  1:565326/1‑139 (MQ=255)        gcgcgcCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACgcgc                                                                                                                                >  2:373323/1‑139 (MQ=255)         cgcgcCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCt                                                                                                                               >  2:352275/1‑139 (MQ=255)                   gCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGcc                                                                                                                     <  2:748168/139‑1 (MQ=255)                         tGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  1:601840/139‑1 (MQ=255)                           cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAc                                                                                                                                    <  1:627940/116‑1 (MQ=255)                           cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAAc                                                                                                                                    >  2:627940/1‑116 (MQ=255)                           cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  1:213418/139‑1 (MQ=255)                           cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             >  2:483527/1‑139 (MQ=255)                           cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  1:479666/139‑1 (MQ=255)                           cGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  2:565326/139‑1 (MQ=255)                             tttAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAgg                                                                                                           <  1:622734/139‑1 (MQ=255)                                            cAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAgcgcg                                                                                            >  1:322853/1‑139 (MQ=255)                                                           ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCccac                                                                                                                  >  1:418324/1‑102 (MQ=255)                                                           ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCccac                                                                                                                  <  2:418324/102‑1 (MQ=255)                                                           ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             >  1:425029/1‑107 (MQ=255)                                                           ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  2:425029/107‑1 (MQ=255)                                                           ccGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcg                                                                             >  1:569539/1‑139 (MQ=255)                                                              aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGTGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcg                                                                          <  2:117467/139‑1 (MQ=255)                                                              aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               >  1:429902/1‑102 (MQ=255)                                                              aTGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcag                                                                                                               <  2:429902/102‑1 (MQ=255)                                                               tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  2:569539/139‑1 (MQ=255)                                                               tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  1:483527/139‑1 (MQ=255)                                                               tGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  1:606515/139‑1 (MQ=255)                                                                       cGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATaa                                                                 <  1:352275/139‑1 (MQ=255)                                                                         cACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAAc                                                               >  2:285111/1‑139 (MQ=255)                                                                               cTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGc                                                         <  2:383364/139‑1 (MQ=255)                                                                                    aGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  2:556265/118‑1 (MQ=255)                                                                                    aGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         >  1:556265/1‑118 (MQ=255)                                                                                       aaGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                                 <  1:288795/139‑1 (MQ=255)                                                                                       aaGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                                 <  1:285111/139‑1 (MQ=255)                                                                                        agagaaTTATGGGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCGCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGAGATAAACCAGTGCAGGTGCGaa                                                >  2:448122/6‑139 (MQ=255)                                                                                        aGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGaa                                                >  1:106586/1‑139 (MQ=255)                                                                                         gTGGATTATGCGCAGTGGTGTGGTACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         <  2:152534/113‑1 (MQ=255)                                                                                         gTGGATTATGCGCAGTGGTGTGGTACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGAcggcgg                                                                         >  1:152534/1‑113 (MQ=255)                                                                                         gTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGaaa                                               >  1:576616/1‑139 (MQ=255)                                                                                         gTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGaaa                                               >  1:85107/1‑139 (MQ=255)                                                                                          tGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                                 <  2:772418/136‑1 (MQ=255)                                                                                          tGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGa                                                 >  1:772418/1‑136 (MQ=255)                                                                                                 tGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAAcc                                       <  2:576616/139‑1 (MQ=255)                                                                                                           tgtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                                    <  2:283613/52‑1 (MQ=255)                                                                                                           tgtgGCACATATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                                    >  1:283613/1‑52 (MQ=255)                                                                                                                  caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  2:385404/52‑1 (MQ=255)                                                                                                                  caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             >  1:385404/1‑52 (MQ=255)                                                                                                                  caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTAccc                        >  1:627369/1‑137 (MQ=255)                                                                                                                  caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTAccc                        <  2:627369/137‑1 (MQ=255)                                                                                                                  caTATTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCcca                      >  1:557590/1‑139 (MQ=255)                                                                                                                   atatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCccac                     <  2:557590/139‑1 (MQ=255)                                                                                                                    tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                                    <  2:742342/43‑1 (MQ=255)                                                                                                                    tatTCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGccc                                                                                                                    >  1:742342/1‑43 (MQ=255)                                                                                                                      ttCCACAGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCccaccac                  <  1:373323/139‑1 (MQ=255)                                                                                                                         cacaGTTGAAGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAg               >  1:106191/1‑139 (MQ=255)                                                                                                                                gAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             >  1:39368/1‑38 (MQ=255)                                                                                                                                gAAGGATTAACCAACGCGCTTCACATCGCCCACcagca                                                                                                             <  2:39368/38‑1 (MQ=255)                                                                                                                                 aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAAcgcg                                                                                <  2:621834/66‑1 (MQ=255)                                                                                                                                 aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAAcgcg                                                                                >  1:621834/1‑66 (MQ=255)                                                                                                                                 aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACgg       >  2:182477/1‑139 (MQ=255)                                                                                                                                 aaGGATTAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACgg       >  2:238181/1‑139 (MQ=255)                                                                                                                                      ttAACCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATg  <  2:106586/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CTTACTGGCGCGCCACCCGCAAACTGCGTTTAAAACTCTTCGGCAAGATAGTAAAAAGCCGATGCCTGACCGCACCGGCTTTAAGAAAGTGGATTATGCGCAGTGGTGTGGCACATATTCCACAGTTGAAGGATTAGCCAACGCGCTTCACATCGCCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATG  >  NC_000913/3870305‑3870576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑