Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,933,819 C→T F14F (TTC→TTT)  rbsA → D‑ribose ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,933,8190CT100.0% 92.3 / NA 30F14F (TTC→TTT) rbsAD‑ribose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/12);  total (18/12)

AACCGCAGAAAGTCAGGCGGTAATTCGCAGCGGAGAATGTTCTCCGTATGCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTCCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTGGCATCTATACTCGCGATGCCG  >  NC_000913/3933686‑3933952
                                                                                                                                     |                                                                                                                                     
aaCCGCAGAAAGTCAGGCGGTAATTCGCAGCGGAGAATGTTCTCCGTATGCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCggg                                                                                                                                  >  2:270739/1‑139 (MQ=255)
      aGAAAGTCAGGCGGTAATTCGCAGCGGAGAATGTTCTCCGTATGCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTaaa                                                                                                                            >  1:33165/1‑139 (MQ=255)
                                             gTATGCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCcg                                                                                     >  2:73284/1‑139 (MQ=255)
                                                 gCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGtt                                                                                                       <  2:459303/117‑1 (MQ=255)
                                                 gCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGtt                                                                                                       >  1:459303/1‑117 (MQ=255)
                                                      tatCATTCTCTGTGCTGCCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATGAAGCCTTTCCGGGCGTAAAAGCCCTCCCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGgc                                                                            >  2:441003/1‑139 (MQ=255)
                                                           ttCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCtgg                                                                       >  1:126804/1‑139 (MQ=255)
                                                                  tgCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCa                                                                                                            >  1:326590/1‑95 (MQ=255)
                                                                  tgCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCa                                                                                                            <  2:326590/95‑1 (MQ=255)
                                                                  tgCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGa                                                                >  1:314821/1‑139 (MQ=255)
                                                                       gCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACg                                                           >  1:416245/1‑139 (MQ=255)
                                                                       gCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACg                                                           >  1:45391/1‑139 (MQ=255)
                                                                          tGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGgcg                                                        <  1:605268/139‑1 (MQ=255)
                                                                            aCGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGcgcg                                                      >  1:102489/1‑139 (MQ=255)
                                                                            aCGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGcgcg                                                      >  1:153143/1‑139 (MQ=255)
                                                                                      gCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATcca                                            >  2:13207/1‑139 (MQ=255)
                                                                                         gTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATccacca                                         >  1:646223/1‑139 (MQ=255)
                                                                                         gTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATccacca                                         <  2:700210/139‑1 (MQ=255)
                                                                                                 aGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGaaa                                 <  1:73284/139‑1 (MQ=255)
                                                                                                     ttACTTCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGc                             >  1:478853/1‑139 (MQ=255)
                                                                                                          tCAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACt                        <  2:126804/139‑1 (MQ=255)
                                                                                                           cAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGgcg                                                        >  2:762837/1‑106 (MQ=255)
                                                                                                           cAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGgcg                                                        <  1:762837/106‑1 (MQ=255)
                                                                                                           cAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTg                       <  2:345738/139‑1 (MQ=255)
                                                                                                           cAGCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTg                       <  2:344646/139‑1 (MQ=255)
                                                                                                             gCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGt                               <  1:84256/129‑1 (MQ=255)
                                                                                                             gCTTAAAGGCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGt                               >  2:84256/1‑129 (MQ=255)
                                                                                                                    ggCATCGATAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTGGCATCtata              <  1:244390/139‑1 (MQ=255)
                                                                                                                            tAAAGCCTTTCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTGGCATCTATACTCGCGAt      <  1:454228/139‑1 (MQ=255)
                                                                                                                                gCCTTTCCGGGGGTAAAAGCCCTCACGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGATGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTGGCATCTATACTCGCGATGCCg  >  2:265758/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                     
AACCGCAGAAAGTCAGGCGGTAATTCGCAGCGGAGAATGTTCTCCGTATGCGAATATCATTCTCTGTGCTGGCGTGACGTTCTGAGGCCGTCATGGAAGCATTACTTCAGCTTAAAGGCATCGATAAAGCCTTCCCGGGCGTAAAAGCCCTCTCGGGCGCAGCGTTAAATGTCTATCCGGGCCGCGTGATGGCGCTGGTGGGCGAAAACGGCGCGGGTAAATCCACCATGATGAAAGTGCTTACTGGCATCTATACTCGCGATGCCG  >  NC_000913/3933686‑3933952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑