Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,950,420	G→A	*33* (TAG→TAA)	ilvL →	ilvG operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,950,420	0	G	A	100.0%	139.4 / NA	43	*33* (TAG→TAA)	ilvL	ilvG operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/18);  total (25/18)

TTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAAC  >  NC_000913/3950291‑3950554                                                                                                                                  |                                                                                                                                       ttCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGc                                                                                                                               >  1:29568/1‑139 (MQ=255) ttCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGc                                                                                                                               >  2:624814/1‑139 (MQ=255)  tCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGcc                                                                                                                              >  1:370651/1‑139 (MQ=255)       cGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAAc                                                                                                                         <  1:624814/139‑1 (MQ=255)           cAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAAc                                                                                                                     <  2:419176/139‑1 (MQ=255)                        aaGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCa                                                                                                        >  1:63934/1‑139 (MQ=255)                                                     aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  <  1:642076/100‑1 (MQ=255)                                                     aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  >  2:642076/1‑100 (MQ=255)                                                      ttAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCtt                                                                          <  2:60929/139‑1 (MQ=255)                                                              gTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACAt                                                                  >  2:222269/1‑139 (MQ=255)                                                                        gCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCgaggag                                                        >  2:158123/1‑139 (MQ=255)                                                                         cGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGc                                                       >  2:262778/1‑139 (MQ=255)                                                                          gtggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                      <  2:370651/139‑1 (MQ=255)                                                                             gtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGAt                                                                                                                                    <  2:72990/58‑1 (MQ=255)                                                                             gtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGAt                                                                                                                                    >  1:72990/1‑58 (MQ=255)                                                                              tggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACa                                                  >  2:335869/1‑139 (MQ=255)                                                                              tggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACa                                                  >  1:18410/1‑139 (MQ=255)                                                                              tggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACa                                                  <  1:222269/139‑1 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaa                                                                                                   <  2:679838/73‑1 (MQ=255)                                                                                               ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaa                                                                                                   >  1:679838/1‑73 (MQ=255)                                                                                               ccGTGCGGGACTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTaa                                                                        >  2:731124/1‑100 (MQ=255)                                                                                               ccGTGCGGGACTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTaa                                                                        <  1:731124/100‑1 (MQ=255)                                                                                                 gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAACACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAt                               >  1:738546/1‑139 (MQ=255)                                                                                                 gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACTAAAt                               >  2:146520/1‑139 (MQ=255)                                                                                                 gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAt                               >  1:270398/1‑139 (MQ=255)                                                                                                  tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACt                                                                                                                    >  2:559253/1‑53 (MQ=255)                                                                                                  tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACt                                                                                                                    <  1:559253/53‑1 (MQ=255)                                                                                                  tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    <  1:366037/117‑1 (MQ=255)                                                                                                  tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    >  2:366037/1‑117 (MQ=255)                                                                                                     ggggCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg                           <  1:235895/139‑1 (MQ=255)                                                                                                       ggCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTg                                                                               <  2:441680/85‑1 (MQ=255)                                                                                                       ggCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTg                                                                               >  1:441680/1‑85 (MQ=255)                                                                                                         cTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc                       >  1:439820/1‑139 (MQ=255)                                                                                                            cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc                                      <  2:346031/121‑1 (MQ=255)                                                                                                            cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc                                      >  1:346031/1‑121 (MQ=255)                                                                                                            cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa                    >  2:375222/1‑139 (MQ=255)                                                                                                                 ggACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGAccc                                                                                                             <  2:595348/45‑1 (MQ=255)                                                                                                                 ggACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGAccc                                                                                                             >  1:595348/1‑45 (MQ=255)                                                                                                                 ggACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAtt               >  1:584551/1‑139 (MQ=255)                                                                                                                         aaaGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCtt                                                                          >  1:49087/1‑72 (MQ=255)                                                                                                                         aaaGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCtt                                                                          <  2:49087/72‑1 (MQ=255)                                                                                                                              cTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac  <  2:584551/139‑1 (MQ=255)                                                                                                                              cTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac  <  2:29568/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       TTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAAC  >  NC_000913/3950291‑3950554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑