Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,077,469 G→A L7L (TTG→TTA)  yiiD → GNAT family putative N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,077,4690GA100.0% 138.3 / NA 43L7L (TTG→TTA) yiiDGNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/19);  total (24/19)

ACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTGTCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACC  >  NC_000913/4077334‑4077588
                                                                                                                                       |                                                                                                                       
aCTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCa                                                                                                                      >  1:123054/1‑139 (MQ=255)
  tATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACg                                                                                                                    <  2:123054/139‑1 (MQ=255)
    tttCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACggg                                                                                                                  <  2:381231/139‑1 (MQ=255)
      tcgtcgAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGaa                                                                                                                >  2:245713/1‑139 (MQ=255)
       cgtcgAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGaaa                                                                                                               <  2:217695/139‑1 (MQ=255)
              cGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAagaga                                                                                                        >  2:81683/1‑139 (MQ=255)
              cGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAagaga                                                                                                        <  1:62293/139‑1 (MQ=255)
                      tCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCg                                                                                                <  1:452857/139‑1 (MQ=255)
                       cAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                               <  1:81683/139‑1 (MQ=255)
                        aGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGTCAGCTTCCAGGGTTATCACGGGAAAAAAGAGAGCGTATCGCt                                                                                              >  2:626086/1‑139 (MQ=255)
                         gCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTa                                                                                             >  2:443481/1‑139 (MQ=255)
                             gagaTGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGTTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTa                                                                                         <  1:626086/139‑1 (MQ=255)
                              agaTGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTAt                                                                                        <  2:177643/139‑1 (MQ=255)
                                  gAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCAcc                                                                                    >  1:713463/1‑139 (MQ=255)
                                   aaCACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCt                                                                                   >  2:560239/1‑139 (MQ=255)
                                    acacGCAAACAGGACGCTTCGCTGCGGATATGCAGGTCTCGCTCGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTAATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCtt                                                                                  >  1:515972/1‑139 (MQ=255)
                                        gCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCggg                                                                              >  1:32938/1‑139 (MQ=255)
                                         cAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCt                                                                                              <  1:244691/122‑1 (MQ=255)
                                         cAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCt                                                                                              >  2:244691/1‑122 (MQ=255)
                                                 aCGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCGGCTTCCAGGGTTATCACg                                                                                                                    >  1:223253/1‑92 (MQ=255)
                                                 aCGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCGGCTTCCAGGGTTATCACg                                                                                                                    <  2:223253/92‑1 (MQ=255)
                                                    cTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACag                                                                  >  2:604335/1‑139 (MQ=255)
                                                      tCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                               >  1:667045/1‑108 (MQ=255)
                                                      tCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGc                                                                                               <  2:667045/108‑1 (MQ=255)
                                                      tCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACagaa                                                                >  1:555882/1‑139 (MQ=255)
                                                        gCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTAt                                                                                                  <  1:39801/103‑1 (MQ=255)
                                                        gCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTAt                                                                                                  >  2:39801/1‑103 (MQ=255)
                                                                 atGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTaga                                                     >  2:299131/1‑139 (MQ=255)
                                                                 atGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTaga                                                     <  1:299131/139‑1 (MQ=255)
                                                                                 tCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGttt                                     >  1:104378/1‑139 (MQ=255)
                                                                                   aaTGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCg                                   <  1:560239/139‑1 (MQ=255)
                                                                                          gCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGG‑TCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGaaa                           >  2:681589/1‑139 (MQ=255)
                                                                                           ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCt                                                                                              <  1:585563/72‑1 (MQ=255)
                                                                                           ccccGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCt                                                                                              >  2:585563/1‑72 (MQ=255)
                                                                                                    aTTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACa                                                                                                             >  1:718378/1‑48 (MQ=255)
                                                                                                    aTTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACa                                                                                                             <  2:718378/48‑1 (MQ=255)
                                                                                                    aTTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTa                  <  2:573366/139‑1 (MQ=255)
                                                                                                         ggTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAg                                        >  1:768639/1‑112 (MQ=255)
                                                                                                         ggTTGCAGGTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAg                                        <  2:768639/112‑1 (MQ=255)
                                                                                                                ggTATGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATc      >  1:607609/1‑139 (MQ=255)
                                                                                                                    tGAGCCAGCTTCCAGGGTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAAcc  >  2:302851/1‑139 (MQ=255)
                                                                                                                                  gggTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTagag                                                    <  2:57958/75‑1 (MQ=255)
                                                                                                                                  gggTTATCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTagag                                                    >  1:57958/1‑75 (MQ=255)
                                                                                                                                       |                                                                                                                       
ACTATTTCGTCGAACGCTGCCGTCAGCAAGAGATGAACACGCAAACAGGACGCTTCGCTGCGGATATGCAGGTATCGCTGGTCAATGATGGCCCCGTGACATTCTGGTTGCAGGTATGAGCCAGCTTCCAGGGTTGTCACGGGAAACAAGAGAGAGTATCGCTATGTATCACCTTCGGGTTCCACAAACAGAAGAAGAATTAGAGCGTTACTATCAGTTTCGCTGGGAAATGTTGCGTAAGCCCCTGCATCAACC  >  NC_000913/4077334‑4077588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑