Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,204,645 G→A *442* (TAG→TAA
N429N (AAC→AAT
zraR →
purD ←
fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,204,6450GA100.0% 80.3 / NA 26*442* (TAG→TAA
N429N (AAC→AAT
zraR
purD
fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/13);  total (13/13)

TTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCA  >  NC_000913/4204523‑4204776
                                                                                                                          |                                                                                                                                   
ttGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGa                                                                                                                     >  1:487846/1‑139 (MQ=255)
                          tCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCgg                                                                                           >  2:268920/1‑139 (MQ=255)
                                         gaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCACAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCGGGCAGAAGCAGTCGTcc                                                                            <  2:441191/139‑1 (MQ=255)
                                               gggCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCgg                                                                                           <  1:36614/118‑1 (MQ=255)
                                               gggCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCgg                                                                                           >  2:36614/1‑118 (MQ=255)
                                                   ggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGatat                                                                  <  2:478060/139‑1 (MQ=255)
                                                     caacaaAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                                <  1:296195/139‑1 (MQ=255)
                                                                                  ggATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTgcgc                                   >  1:700324/1‑139 (MQ=255)
                                                                                      cACGCGCAAAGCGGAATTAGCAAAACTGTCGCGTTAAAGCTGCACGCGTTCGATAGCGACACAGCCGCTGTCTTTCCGGCAGAATCAGTCGTCCCAGTGGATATCGGTCATAAAGGCATAGGCGAGTTTCTGCGCTTCt                               >  2:148347/1‑139 (MQ=255)
                                                                                       aCGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGtt                                                                                                                        >  1:604803/1‑49 (MQ=255)
                                                                                       aCGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGtt                                                                                                                        <  2:604803/49‑1 (MQ=255)
                                                                                        cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTccgc                                                                                                                               <  1:261235/41‑4 (MQ=255)
                                                                                        cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                            >  2:427624/1‑92 (MQ=255)
                                                                                        cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                            <  1:427624/92‑1 (MQ=255)
                                                                                        cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                             >  1:677348/1‑139 (MQ=255)
                                                                                        cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                             >  2:311379/1‑139 (MQ=255)
                                                                                        cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                             >  2:767579/1‑139 (MQ=255)
                                                                                        cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCCGc                                                                                                                               >  2:261235/1‑41 (MQ=255)
                                                                                         gcgcAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGTGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                            <  2:48812/139‑1 (MQ=255)
                                                                                          cgcAAAACGCTATTGGCAAAACTGTCGCATTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                           <  2:680973/139‑1 (MQ=255)
                                                                                                     aTTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGAccc                >  1:768366/1‑139 (MQ=255)
                                                                                                       tGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAg              <  2:609899/139‑1 (MQ=255)
                                                                                                       tGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAg              <  1:268920/139‑1 (MQ=255)
                                                                                                         gCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGAGAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg            <  1:661759/139‑1 (MQ=255)
                                                                                                                       ttAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca  <  1:668503/135‑1 (MQ=255)
                                                                                                                       ttAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca  >  2:668503/1‑135 (MQ=255)
                                                                                                                          |                                                                                                                                   
TTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCA  >  NC_000913/4204523‑4204776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: