Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,292,149 A→G Y213C (TAC→TGC)  nrfE → heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,292,1490AG100.0% 80.2 / NA 24Y213C (TAC→TGC) nrfEheme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/14);  total (10/14)

TGGCGCTGGCGAGTCTACTGCGCGGCGAGTTTGATGGTGCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTACTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTGGCTTTCTGCCACTGCGCTGCTGCACAGTTTGTCCCTGACACGCCAGCGGGGGATTTTTTGCCACTGGT  >  NC_000913/4292026‑4292283
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tGGCGCTGGCGAGTCTACTGCGCGGCGAGTTTGATGGTGCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTg                                                                                                                         <  2:557723/139‑1 (MQ=255)
     cTGGCGAGTCTACTGCGCGGCGAGTTTGATGGTGCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGggcg                                                                                                                    <  2:569941/139‑1 (MQ=255)
          gAGTCTACTGCGCGGCGAGTTTGATGGTGCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCtgg                                                                                                               <  1:701347/139‑1 (MQ=255)
            gTCTACTGCGCGGCGAGTTTGATGGTGCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCtggtg                                                                                                             <  2:419432/139‑1 (MQ=255)
                    cgcgGCGAGTTTGATGGTGCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTATCTGGCTGGAGTGCATTAACGGCGCGGATCATCCTCGGTTGCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTggg                                                                                                     <  1:490892/139‑1 (MQ=255)
                         cgAGTTTGATGGTGCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCt                                                                                                                                      >  1:291947/1‑101 (MQ=255)
                         cgAGTTTGATGGTGCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCt                                                                                                                                      <  2:291947/101‑1 (MQ=255)
                                    gTGCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGc                                                                                     <  2:346240/139‑1 (MQ=255)
                                      gCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCct                                                                                   <  1:67413/139‑1 (MQ=255)
                                                  aTTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCt                                                                       <  2:302870/139‑1 (MQ=255)
                                                        tGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTgg                                                                     >  2:90096/1‑135 (MQ=255)
                                                        tGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTgg                                                                     <  1:90096/135‑1 (MQ=255)
                                                                               gAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTGGCTTTCTGCCACTGCgctg                                                   >  2:85857/1‑130 (MQ=255)
                                                                               gAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTGGCTTTCTGCCACTGCgctg                                                   <  1:85857/130‑1 (MQ=255)
                                                                               gAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTGGCTTTCTGCCACTGCGCTGCTGCACAGt                                          >  1:174577/1‑139 (MQ=255)
                                                                               gAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGAGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTGGCCTTCTGCCACTGCGCTGCTGCACAGt                                          >  1:513590/1‑139 (MQ=255)
                                                                                      ttAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTgg                                                                     >  2:233403/1‑105 (MQ=255)
                                                                                      ttAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTgg                                                                     <  1:233403/105‑1 (MQ=255)
                                                                                         aCGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCctc                                                                                  >  2:39806/1‑89 (MQ=255)
                                                                                         aCGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCctc                                                                                  <  1:39806/89‑1 (MQ=255)
                                                                                                       ccTCGGTTCCTGGTGGGCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTGGCTTTCTGCCACTGCGCTGCTGCACAGTTTGTCCCTGACACGCCAGCggggg                  >  2:704387/1‑139 (MQ=255)
                                                                                                                       gCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTgg                                                                     >  2:230741/1‑72 (MQ=255)
                                                                                                                       gCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTgg                                                                     <  1:230741/72‑1 (MQ=255)
                                                                                                                       gCCTGCTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTGGCTTTCTGCCACTGCGCTGCTGCACAGTTTGTCCCTGACACGCCAGCGGGGGATTTTTTGCCACTGGt  >  1:140483/1‑139 (MQ=255)
                                                                                                                           |                                                                                                                                      
TGGCGCTGGCGAGTCTACTGCGCGGCGAGTTTGATGGTGCCTGCGCCCGAATTTGCTGGCGCTGGGCGTTACCTGGCTGGAGTGCATTAACGGCGGGGATCATCCTCGGTTCCTGGTGGGCCTACTGCGAACTCGGCTGGGGCGGCTGGTGGTTCTGGGATCCGGTGGAAAACGCCTCTTTATTACCCTGGCTTTCTGCCACTGCGCTGCTGCACAGTTTGTCCCTGACACGCCAGCGGGGGATTTTTTGCCACTGGT  >  NC_000913/4292026‑4292283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: