| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,605,605 | G→A | P20L (CCG→CTG) | fhuF ← | ferric iron reductase involved in ferric hydroximate transport |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,605,605 | 0 | G | A | 98.1% | 166.2 / ‑5.3 | 52 | P20L (CCG→CTG) | fhuF | ferric iron reductase involved in ferric hydroximate transport |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/29); minor base C (0/1); total (22/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACATTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTAT > NC_000913/4605469‑4605736 | cacaTTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGTGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGc > 1:663900/1‑139 (MQ=255) tCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGgag > 2:286040/1‑139 (MQ=255) tCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGgag < 2:769207/139‑1 (MQ=255) tgatgaCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGc > 1:699830/1‑139 (MQ=255) tgaCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGtt < 2:7919/139‑1 (MQ=255) gaCCATTTCGCCAGCGTCATGGCGTTAAGTGGTGCAGGTTCATCCAGTCGGATAAACTCGAGCAAATGTTCACGATGATTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTc > 2:488400/1‑139 (MQ=255) gaCCATTGCGCCAGCGTCATGGCGTTAAGGGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTc > 2:595995/1‑139 (MQ=255) aCCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCg > 1:723282/1‑139 (MQ=255) tGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGa > 2:614762/1‑139 (MQ=255) cgcCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATg > 2:512601/1‑139 (MQ=255) gcCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGa < 1:614762/139‑1 (MQ=255) gcCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGa < 2:558485/139‑1 (MQ=255) aGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACAt < 1:404525/139‑1 (MQ=255) cGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATagag < 2:721029/139‑1 (MQ=255) cGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATagag < 2:699830/139‑1 (MQ=255) aGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTg > 2:229250/1‑139 (MQ=255) aGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGa < 2:184958/139‑1 (MQ=255) ggTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGAt < 1:455925/139‑1 (MQ=255) gTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCCGCTGGATATGCGTTCGCCAGATTACATCTTCATAGAGCGGTGCGGAACGATa < 2:445955/139‑1 (MQ=255) gTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATa > 2:7267/1‑139 (MQ=255) tCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAgg > 2:134575/1‑139 (MQ=255) tCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCa > 2:553377/1‑139 (MQ=255) ggCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAAt < 1:387420/139‑1 (MQ=255) gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACAt < 1:367019/104‑1 (MQ=255) gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACAt > 2:367019/1‑104 (MQ=255) gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATa > 1:545514/1‑111 (MQ=255) gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATc > 1:492111/1‑139 (MQ=255) gCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTCGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATa < 2:545514/111‑1 (MQ=255) tAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGAt < 1:553377/139‑1 (MQ=255) aaaCTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATa < 1:7267/139‑1 (MQ=255) cTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTa < 1:90019/139‑1 (MQ=255) ccAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAAt < 1:286040/139‑1 (MQ=255) ccAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAAt < 1:282725/139‑1 (MQ=255) aGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCt < 2:723282/139‑1 (MQ=255) cACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataat < 1:134575/139‑1 (MQ=255) cGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataatga > 2:396282/1‑139 (MQ=255) cGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAaatgataatga < 2:240599/139‑1 (MQ=255) gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGTGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCg > 1:579548/1‑139 (MQ=255) gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGa > 2:336504/1‑87 (MQ=255) gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGa < 1:336504/87‑1 (MQ=255) gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCg > 2:281165/1‑139 (MQ=255) gtcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCg < 1:512601/139‑1 (MQ=255) tcgCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGa < 2:398904/139‑1 (MQ=255) cACGGCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGttt > 1:357271/1‑139 (MQ=255) gCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACa > 2:208258/1‑51 (MQ=255) gCTTGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACa < 1:208258/51‑1 (MQ=255) tGCGCAAGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGTGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGAt < 2:579548/139‑1 (MQ=255) aaGCGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCaa < 1:77838/139‑1 (MQ=255) cGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGAt < 2:592336/81‑1 (MQ=255) cGTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGAt > 1:592336/1‑81 (MQ=255) gTGGGATCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGa < 2:492111/139‑1 (MQ=255) aTCCTGCAGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTAt < 1:590804/139‑1 (MQ=255) | CACATTCGGTGATGACCATTGCGCCAGCGTCATGGCGTTAAGTGGGGCAGGTTCATCCAGGCGGATAAACTCCAGCAAATGTTCACGATGTTTTGCGATCGTCGCCCGCACGGCTTGCGCAAGCGTGGGATCCTGCGGCTGGAGATGCGTTCGCCAGATGACATCTTCATAGAGCGGTGCGGAACGATAGGCCATAATCGGGATAGTAATCTAAATGATAATGATTGCTAATCATAGCGATAGGTTTACCCGATAGCAAGGGATTTAT > NC_000913/4605469‑4605736 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |