Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 634,746 2 bp→AT [ybdL][ybdM] [ybdL], [ybdM]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913634,7460GA100.0% 59.9 / NA 20*387* (TAG→TAAybdLmethionine aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/12);  total (8/12)
*NC_000913634,7470CT100.0% 61.3 / NA 20*210* (TAG→TAAybdMSpo0J family protein, ParB‑like nuclease domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/12);  total (8/12)

AGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCC  >  NC_000913/634616‑634857
                                                                                                                                  ||                                                                                                              
aGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTaa                                                                                                         <  1:171856/139‑1 (MQ=255)
    cGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGt                                                                                                     >  2:233619/1‑139 (MQ=255)
              gcgATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCgg                                                                                           <  2:368850/139‑1 (MQ=255)
                            ggTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTc                                                                             <  1:508695/139‑1 (MQ=255)
                             gtgtTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCg                                                                            <  1:27767/139‑1 (MQ=255)
                                      gcCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAaacaac                                                                   <  1:751356/139‑1 (MQ=255)
                                        cGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAActc                                                                 >  1:495582/1‑139 (MQ=255)
                                                           cTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGc                                              >  1:227809/1‑139 (MQ=255)
                                                                   tctcTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGgcgc                                                                                             >  1:313735/1‑84 (MQ=255)
                                                                   tctcTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGgcgc                                                                                             <  2:313735/84‑1 (MQ=255)
                                                                           ttGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACtt                              <  1:233619/139‑1 (MQ=255)
                                                                              ccAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGAc                                                                                 >  2:548913/1‑85 (MQ=255)
                                                                              ccAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGAc                                                                                 <  1:548913/85‑1 (MQ=255)
                                                                                        aTCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCAt                 >  1:42876/1‑139 (MQ=255)
                                                                                            aCGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTccc             <  2:42876/139‑1 (MQ=255)
                                                                                                      cagcagCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGc   <  2:495582/139‑1 (MQ=255)
                                                                                                                       cgcCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGAc                                                                                 >  2:230604/1‑44 (MQ=255)
                                                                                                                       cgcCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGAc                                                                                 <  1:230604/44‑1 (MQ=255)
                                                                                                                         ccAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGcc  >  2:687274/1‑121 (MQ=255)
                                                                                                                         ccAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGcc  <  1:687274/121‑1 (MQ=255)
                                                                                                                                  ||                                                                                                              
AGCACGGCGTAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCC  >  NC_000913/634616‑634857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: