Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,208,534 Δ1 bp coding (403/414 nt);coding (586/603 nt) tfaP →
tfaE ←
e14 prophage, uncharacterized protein
e14 prophage, putative tail fiber assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,208,5340C.100.0% 256.2 / NA 71coding (403/414 nt)
coding (586/603 nt)
tfaP
tfaE
e14 prophage, uncharacterized protein
e14 prophage, putative tail fiber assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (39/32);  total (39/32)

GAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGTTGAATCA  >  NC_000913/1208415‑1208665
                                                                                                                       |                                                                                                                                   
gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                                                 <  2:1133319/139‑1 (MQ=255)
 aaGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcgc                                                                                                                <  1:1502704/139‑1 (MQ=255)
    aCTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGt                                                                                                             >  2:921083/1‑139 (MQ=255)
         aagaagATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTgg                                                                                                        >  1:1497886/1‑139 (MQ=255)
              gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATc                                                                                                                      >  1:1064543/1‑120 (MQ=255)
              gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATc                                                                                                                      <  2:1064543/120‑1 (MQ=255)
              gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCt                                                                                                   <  2:98396/139‑1 (MQ=255)
               aTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTg                                                                                                  >  2:1318296/1‑139 (MQ=255)
                        aaCAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                                                 >  2:22605/1‑115 (MQ=255)
                        aaCAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                                                 <  1:22605/115‑1 (MQ=255)
                                    tgtgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGt                                                                             >  1:828180/1‑139 (MQ=255)
                                     gtgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATg                                                                                                                           <  1:1147371/92‑1 (MQ=255)
                                     gtgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATg                                                                                                                           >  2:1147371/1‑92 (MQ=255)
                                      tgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                              <  2:394554/136‑1 (MQ=255)
                                      tgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                              >  1:394554/1‑136 (MQ=255)
                                       ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAAt                                                                          >  1:1190948/1‑139 (MQ=255)
                                                aTCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAg                                                                 >  1:1520818/1‑139 (MQ=255)
                                                      aaGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                              >  2:229679/1‑120 (MQ=255)
                                                      aaGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                              <  1:229679/120‑1 (MQ=255)
                                                           cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGtt                                                             <  1:816101/132‑1 (MQ=255)
                                                           cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGtt                                                             >  2:816101/1‑132 (MQ=255)
                                                           cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTg                                                      >  2:684488/1‑139 (MQ=255)
                                                            tGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                                                   >  1:1318394/1‑109 (MQ=255)
                                                            tGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                                                   <  2:1318394/109‑1 (MQ=255)
                                                             ggAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGgt                                                    >  2:309696/1‑139 (MQ=255)
                                                                  tGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGttt                                               <  2:1520818/139‑1 (MQ=255)
                                                                    gTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGt                                                                                                             >  2:1373422/1‑75 (MQ=14)
                                                                    gTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGt                                                                                                             <  1:1373422/75‑1 (MQ=17)
                                                                     ttGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                              >  2:1639423/1‑105 (MQ=255)
                                                                     ttGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                              <  1:1639423/105‑1 (MQ=255)
                                                                     ttGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTctg                                            >  2:849700/1‑139 (MQ=255)
                                                                            aTCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                                     <  2:1497886/139‑1 (MQ=255)
                                                                            aTCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                                     <  1:684488/139‑1 (MQ=255)
                                                                             tCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTc                                    >  2:1570534/1‑139 (MQ=255)
                                                                              ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                              <  1:1025049/96‑1 (MQ=255)
                                                                              ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCATCACCGCGTCAATg                                                                              >  2:1025049/1‑96 (MQ=11)
                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCGGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGttt                               >  2:471982/1‑139 (MQ=255)
                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGt                                                                                                       >  2:621171/1‑67 (MQ=11)
                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGt                                                                                                       <  1:621171/67‑1 (MQ=11)
                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                              >  2:153653/1‑92 (MQ=255)
                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                                              <  1:153653/92‑1 (MQ=255)
                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTctgcc                                          >  1:1105718/1‑128 (MQ=255)
                                                                                  tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTctgcc                                          <  2:1105718/128‑1 (MQ=255)
                                                                                    cgcCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGtt                                >  1:1504181/1‑136 (MQ=255)
                                                                                    cgcCAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGtt                                <  2:1504181/136‑1 (MQ=255)
                                                                                      ccAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                                        <  2:1048035/78‑1 (MQ=11)
                                                                                      ccAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                                        >  1:1048035/1‑78 (MQ=11)
                                                                                      ccAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                                     >  2:831036/1‑129 (MQ=255)
                                                                                      ccAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                                     <  1:831036/129‑1 (MQ=255)
                                                                                      ccAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGt                           >  1:737497/1‑139 (MQ=255)
                                                                                       cAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                                                 >  1:1281454/1‑52 (MQ=11)
                                                                                       cAGATATTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                                                 <  2:1281454/52‑1 (MQ=11)
                                                                                          atatTGAATGGCTTACGCGTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCgg                       >  2:960755/1‑139 (MQ=255)
                                                                                          atatTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCgg                       <  1:832042/139‑1 (MQ=255)
                                                                                          atatTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCgg                       <  1:921083/139‑1 (MQ=255)
                                                                                          atatTGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGCTTCTGCCTGCGTCAGTTTACGTCCgg                       >  2:1100937/1‑139 (MQ=255)
                                                                                              tGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                                                   >  1:750863/1‑75 (MQ=11)
                                                                                              tGAATGGCTTACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                                                   <  2:750863/75‑1 (MQ=11)
                                                                                                    gcttACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAAttt             >  2:839664/4‑139 (MQ=255)
                                                                                                     cttACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                                                 <  2:614091/36‑1 (MQ=14)
                                                                                                     cttACGCCTCCGGCAGTTAGGCCAGATGACATCCGgcg                                                                                                                 >  1:614091/3‑38 (MQ=14)
                                                                                                     cttACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                                        <  2:1552607/61‑1 (MQ=11)
                                                                                                     cttACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                                        >  1:1552607/3‑63 (MQ=11)
                                                                                                     cttACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGcac                                                                   <  2:1640274/82‑1 (MQ=255)
                                                                                                     cttACGCCTCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGcac                                                                   >  1:1640274/3‑84 (MQ=255)
                                                                                                             tCCGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGTTGAAt    <  1:849700/139‑1 (MQ=255)
                                                                                                              ccGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGtt                                <  1:1383210/110‑1 (MQ=255)
                                                                                                              ccGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGtt                                >  2:1383210/1‑110 (MQ=255)
                                                                                                              ccGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGt         >  1:200583/1‑133 (MQ=255)
                                                                                                              ccGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGt         <  2:200583/133‑1 (MQ=255)
                                                                                                               cGGCAGTTAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGTTGAATCa  >  2:170781/1‑139 (MQ=255)
                                                                                                                       |                                                                                                                                   
GAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGGCCTGCAATTTCAGTTGAATCA  >  NC_000913/1208415‑1208665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: