Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide and tripeptide permease A/glutathionine S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,714,3180T.98.2% 211.2 / ‑0.9 56intergenic (+47/‑59)dtpA/gstAdipeptide and tripeptide permease A/glutathionine S‑transferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (25/29);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

ATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAA  >  NC_000913/1714191‑1714444
                                                                                                                               |                                                                                                                              
aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttttgttc                                                                                                                     >  2:454368/1‑135 (MQ=255)
    tgctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCa                                                                                                                <  2:1621934/139‑1 (MQ=255)
     gctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCa                                                                                                                >  1:1306390/1‑138 (MQ=255)
     gctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCa                                                                                                                <  2:1306390/138‑1 (MQ=255)
       tgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACg                                                                                                             <  1:1164618/139‑1 (MQ=255)
           cgcgCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttttgtt                                                                                                                      >  2:614690/1‑124 (MQ=255)
           cgcgCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttttgtt                                                                                                                      <  1:614690/127‑4 (MQ=255)
              gcCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTAcc                                                                                                      <  1:718630/139‑1 (MQ=255)
                  aaaCTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCa                                                                                                     >  2:400106/1‑136 (MQ=255)
                  aaaCTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCa                                                                                                     <  1:400106/136‑1 (MQ=255)
                                                           ggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTc                                                          >  2:527877/1‑139 (MQ=255)
                                                           ggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                                                       <  2:1729968/109‑1 (MQ=255)
                                                           ggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                                                       <  1:1721652/109‑1 (MQ=255)
                                                           ggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                                                       >  1:1729968/1‑109 (MQ=255)
                                                           ggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                                                       >  2:1721652/1‑109 (MQ=255)
                                                           ggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑‑TTTTTTTGTTCAGCAACg                                                                                                             >  1:950442/1‑86 (MQ=255)
                                                           ggcgAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑‑TTTTTTTGTTCAGCAACg                                                                                                             <  2:950442/86‑1 (MQ=255)
                                                                   cagcCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCg                                                 <  1:571098/139‑1 (MQ=255)
                                                                   cagcCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCg                                                 >  2:571098/1‑139 (MQ=255)
                                                                           gCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATGCTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAAcc                                                  <  1:122406/130‑1 (MQ=255)
                                                                           gCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATGCTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAAcc                                                  >  2:122406/1‑130 (MQ=255)
                                                                           gCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTg                                         >  1:152620/1‑139 (MQ=255)
                                                                            cGTAAAATTCAGGGAAACTCTTTTACAAGTCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGc                                        >  1:1157963/1‑139 (MQ=255)
                                                                            cGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTAcc                                                                                                      >  2:1274772/1‑77 (MQ=255)
                                                                            cGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTAcc                                                                                                      <  1:1274772/77‑1 (MQ=255)
                                                                             gTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGct                                       <  2:1022504/139‑1 (MQ=255)
                                                                             gTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGCCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGATGCCTGct                                       >  1:182948/1‑139 (MQ=255)
                                                                              tAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGctc                                      >  1:1597309/1‑139 (MQ=255)
                                                                                aaaTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACg                                                                                                             >  2:661762/1‑66 (MQ=255)
                                                                                aaaTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACg                                                                                                             <  1:661762/66‑1 (MQ=255)
                                                                                  aTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTATGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCtctctc                                  <  2:1157963/139‑1 (MQ=255)
                                                                                       gggAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTc                             <  1:454368/139‑1 (MQ=255)
                                                                                        ggAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGtt                                                                          >  2:1385907/1‑93 (MQ=255)
                                                                                        ggAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGtt                                                                          <  1:1385907/93‑1 (MQ=255)
                                                                                             ctctTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACa                                                      >  1:1582283/1‑108 (MQ=255)
                                                                                             ctctTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACa                                                      <  2:1582283/108‑1 (MQ=255)
                                                                                                  ttACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAg                                                                                                                  <  2:1620396/43‑1 (MQ=255)
                                                                                                  ttACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAg                                                                                                                  >  1:1620396/1‑43 (MQ=255)
                                                                                                  ttACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGc                                                                                                            <  1:1083254/49‑1 (MQ=255)
                                                                                                  ttACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGc                                                                                                            >  2:1083254/1‑49 (MQ=255)
                                                                                                  ttACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCa                                                                                                     <  1:1485575/56‑1 (MQ=255)
                                                                                                  ttACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCa                                                                                                     >  2:1485575/1‑56 (MQ=255)
                                                                                                    aCAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTg                <  2:152620/139‑1 (MQ=255)
                                                                                                        gCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCa                                                                                                           <  1:1035433/44‑1 (MQ=38)
                                                                                                        gCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                                                       <  1:1600854/64‑1 (MQ=255)
                                                                                                        gCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                                                       >  2:1600854/1‑64 (MQ=255)
                                                                                                        gCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTc                             >  2:1539118/1‑122 (MQ=255)
                                                                                                        gCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTc                             <  1:1539118/122‑1 (MQ=255)
                                                                                                        gCCGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTg            >  2:298958/1‑139 (MQ=255)
                                                                                                        gCCGCTAACTTTTCGTTAGCGGCTTTTATTTGTTCAGCAACGCa                                                                                                           >  2:1035433/1‑44 (MQ=255)
                                                                                                          cGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGc                                                                                                                 <  1:762701/36‑1 (MQ=255)
                                                                                                          cGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGc                                                                                                                 >  2:762701/1‑36 (MQ=255)
                                                                                                          cGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                                                                         <  1:1503478/60‑1 (MQ=255)
                                                                                                          cGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACca                                                                                         >  2:1503478/1‑60 (MQ=255)
                                                                                                          cGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCt                               >  1:1344592/1‑118 (MQ=255)
                                                                                                          cGCTAACTTTTCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCt                               <  2:1344592/118‑1 (MQ=255)
                                                                                                                  tttCGTTAGCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGaaa  <  1:298958/139‑1 (MQ=255)
                                                                                                                          gCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTAc                                                       >  2:555960/1‑78 (MQ=255)
                                                                                                                          gCGGCTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTAc                                                       <  1:555960/78‑1 (MQ=255)
                                                                                                                               |                                                                                                                              
ATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAAGCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAA  >  NC_000913/1714191‑1714444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: