Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,821,867 (A)8→7 intergenic (‑248/+51) chbB ← / ← osmE N,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,821,8600A.100.0% 387.7 / NA 94intergenic (‑241/+58)chbB/osmEN,N'‑diacetylchitobiose‑specific enzyme IIB component of PTS/osmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (49/45);  total (49/45)

TATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NC_000913/1821735‑1821994
                                                                                                                             |                                                                                                                                      
tataCGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGgcgc                                                                                                                          >  1:629234/1‑139 (MQ=255)
                 aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCg                                                                                                                  <  1:778647/130‑1 (MQ=255)
                 aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCg                                                                                                                  >  2:778647/1‑130 (MQ=255)
                 aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:543841/1‑139 (MQ=255)
                 aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:894542/1‑139 (MQ=255)
                 aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:130837/1‑139 (MQ=255)
                   cAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgttgt                                                                                                       <  1:894542/139‑1 (MQ=255)
                         tATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCa                                                                                                 <  2:130837/139‑1 (MQ=255)
                              cTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCtt                                                                                            >  2:1089236/1‑139 (MQ=255)
                                ttGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGc                                                                                                                    >  1:273543/1‑113 (MQ=255)
                                ttGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGc                                                                                                                    <  2:273543/113‑1 (MQ=255)
                                 tGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCACTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCa                                                                                         >  1:1133138/1‑139 (MQ=255)
                                  gACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAg                                                                                        <  2:629234/139‑1 (MQ=255)
                                   aCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaaccg                                                                                                                               <  1:1311140/100‑4 (MQ=255)
                                   aCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaaccg                                                                                                                               >  2:1311140/1‑97 (MQ=255)
                                   aCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGa                                                                                       >  1:166078/1‑139 (MQ=255)
                                   aCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGa                                                                                       >  1:813704/1‑139 (MQ=255)
                                        ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:1512392/1‑116 (MQ=255)
                                        ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                         <  2:1512392/116‑1 (MQ=255)
                                        ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAt                                                                                  >  2:1200301/1‑139 (MQ=255)
                                             ttGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGAtt                                                                             <  2:166078/139‑1 (MQ=255)
                                                tttCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             <  1:556230/104‑1 (MQ=255)
                                                tttCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             >  2:556230/1‑104 (MQ=255)
                                                  tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCaa                                                                                                                        <  1:1468935/91‑1 (MQ=255)
                                                  tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCaa                                                                                                                        >  2:1468935/1‑91 (MQ=255)
                                                   ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  2:543841/139‑1 (MQ=255)
                                                     ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:1089236/139‑1 (MQ=255)
                                                               tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgttg                                                                                                        >  2:884323/1‑94 (MQ=255)
                                                               tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgttg                                                                                                        <  1:884323/94‑1 (MQ=255)
                                                                ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATc                                                          >  1:1374470/1‑139 (MQ=255)
                                                                    aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGgcg                                                                                                                           <  2:1405525/70‑1 (MQ=255)
                                                                    aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGgcg                                                                                                                           >  1:1405525/1‑70 (MQ=255)
                                                                    aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  1:486714/107‑1 (MQ=255)
                                                                    aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      >  2:486714/1‑107 (MQ=255)
                                                                    aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      >  1:843074/1‑139 (MQ=255)
                                                                         ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  2:1658174/117‑1 (MQ=255)
                                                                         ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       >  1:1658174/1‑117 (MQ=255)
                                                                         ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  2:792382/1‑139 (MQ=255)
                                                                           tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGcc                                                                                                                   <  1:427153/71‑1 (MQ=255)
                                                                           tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGcc                                                                                                                   >  2:427153/1‑71 (MQ=255)
                                                                           tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      <  1:1462679/132‑1 (MQ=255)
                                                                           tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      >  2:1462679/1‑132 (MQ=255)
                                                                            ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGt                                                                                                                <  2:171127/73‑1 (MQ=255)
                                                                            ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGt                                                                                                                >  1:171127/1‑73 (MQ=255)
                                                                            ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgttg                                                                                                        <  1:1493304/81‑1 (MQ=255)
                                                                            ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgttg                                                                                                        >  2:1493304/1‑81 (MQ=255)
                                                                            ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc                                                                                                  >  1:1445961/1‑87 (MQ=255)
                                                                            ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTc                                                                                                  <  2:1445961/87‑1 (MQ=255)
                                                                               cacaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGc                                           >  1:527602/1‑139 (MQ=255)
                                                                                acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATTGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:1239983/139‑1 (MQ=255)
                                                                                acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:843074/139‑1 (MQ=255)
                                                                                  aTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGcaca                                        >  1:898199/1‑139 (MQ=255)
                                                                                     tCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGcc                                                                                                                   >  1:1051377/1‑61 (MQ=255)
                                                                                     tCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGcc                                                                                                                   <  2:1051377/61‑1 (MQ=255)
                                                                                      cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             >  2:519721/1‑66 (MQ=255)
                                                                                      cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             <  2:1383833/66‑1 (MQ=255)
                                                                                      cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             >  1:1383833/1‑66 (MQ=255)
                                                                                      cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTc                                                                                                             <  1:519721/66‑1 (MQ=255)
                                                                                      cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTc                                    >  1:1488003/1‑139 (MQ=255)
                                                                                       gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaacc                                                                                                                                <  1:203195/47‑3 (MQ=255)
                                                                                       gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCaaaaaaacc                                                                                                                                >  2:203195/1‑45 (MQ=255)
                                                                                       gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgttg                                                                                                        <  2:643448/70‑1 (MQ=255)
                                                                                       gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCgttg                                                                                                        >  1:643448/1‑70 (MQ=255)
                                                                                       gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTa                                                                                                     >  1:1253197/1‑73 (MQ=255)
                                                                                       gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTa                                                                                                     <  2:1253197/73‑1 (MQ=255)
                                                                                       gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                             >  2:19078/1‑81 (MQ=255)
                                                                                       gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                             <  1:19078/81‑1 (MQ=255)
                                                                                       gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGt                                     <  2:535588/137‑1 (MQ=255)
                                                                                       gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGt                                     >  1:535588/1‑137 (MQ=255)
                                                                                       gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                                   <  1:915366/139‑1 (MQ=255)
                                                                                           aaaTAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATgg                                                                                                                     <  2:943589/53‑1 (MQ=255)
                                                                                           aaaTAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATgg                                                                                                                     >  1:943589/1‑53 (MQ=255)
                                                                                            aataatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATgg                                                                                                                     <  1:1007437/52‑1 (MQ=255)
                                                                                            aataatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATgg                                                                                                                     >  2:1007437/1‑52 (MQ=255)
                                                                                            aataatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGc                                                                                                                    <  1:1693551/53‑1 (MQ=255)
                                                                                            aataatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGc                                                                                                                    >  2:1693551/1‑53 (MQ=255)
                                                                                            aataatTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATaa                              <  1:1200301/139‑1 (MQ=255)
                                                                                                 ttCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGa                         <  2:1488003/139‑1 (MQ=255)
                                                                                                  tCGCGAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAAGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATGACCGGAg                        <  2:1133138/139‑1 (MQ=255)
                                                                                                     cgAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGtt                                                                                                               >  1:542788/1‑49 (MQ=39)
                                                                                                     cgAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGtt                                                                                                               <  2:542788/49‑1 (MQ=39)
                                                                                                     cgAAGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtg                     >  1:207608/1‑139 (MQ=255)
                                                                                                       aaGATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtgat                   <  2:1374470/139‑1 (MQ=255)
                                                                                                         gATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  1:719133/104‑1 (MQ=255)
                                                                                                         gATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    >  2:719133/1‑104 (MQ=255)
                                                                                                         gATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  1:267408/104‑1 (MQ=255)
                                                                                                         gATAATTAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    >  2:267408/1‑104 (MQ=255)
                                                                                                               tAAGAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  2:813704/139‑1 (MQ=255)
                                                                                                                  gAACTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCgg        >  2:96365/1‑139 (MQ=255)
                                                                                                                   aaCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  1:240685/75‑1 (MQ=255)
                                                                                                                   aaCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       >  2:240685/1‑75 (MQ=255)
                                                                                                                    aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  2:1172740/59‑1 (MQ=255)
                                                                                                                    aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      >  1:1172740/1‑59 (MQ=255)
                                                                                                                    aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       >  1:319506/1‑74 (MQ=255)
                                                                                                                    aCTCAGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  2:319506/74‑1 (MQ=255)
                                                                                                                        aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtg                     <  2:695240/120‑1 (MQ=255)
                                                                                                                        aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtg                     >  1:695240/1‑120 (MQ=255)
                                                                                                                        aGGCCAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTctcttataca  >  1:1529832/1‑130 (MQ=255)
                                                                                                                             |                                                                                                                                      
TATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NC_000913/1821735‑1821994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: