Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,869,022	(T)6→5	coding (68/1284 nt)	yeaH →	UPF0229 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,869,017	0	T	.	100.0%	248.0 / NA	61	coding (63/1284 nt)	yeaH	UPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (31/30);  total (31/30)

AGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NC_000913/1868884‑1869150                                                                                                                                      |                                                                                                                                      aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  2:1098857/139‑2 (MQ=255) aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  2:1445410/139‑2 (MQ=255) aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  2:104995/139‑2 (MQ=255) aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  2:1580950/139‑2 (MQ=255)    gAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacgc                                                                                                                               >  2:740634/1‑135 (MQ=255)     aaCCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                <  2:1265584/137‑4 (MQ=255)     aaCCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                >  1:1265584/1‑134 (MQ=255)         gTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTta                                                                                                                         >  2:1209010/1‑139 (MQ=255)               cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc                                                                                                                   <  2:1604837/139‑1 (MQ=255)                 gagaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgcgc                                                                                                                 >  2:1616545/1‑139 (MQ=255)                       cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTa                                                                                                           >  2:427193/1‑139 (MQ=255)                                       gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  1:740634/139‑1 (MQ=255)                                       gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  2:71680/139‑1 (MQ=255)                                         aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:1209010/139‑1 (MQ=255)                                         aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:427193/139‑1 (MQ=255)                                           aCAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGcc                                                                                       <  1:1616545/139‑1 (MQ=255)                                               ttGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  >  1:612869/1‑92 (MQ=255)                                               ttGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  2:612869/93‑2 (MQ=255)                                               ttGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             <  1:421070/129‑1 (MQ=255)                                               ttGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             >  2:421070/1‑129 (MQ=255)                                                  gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  1:995330/1‑139 (MQ=255)                                                       tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                           >  2:1142456/1‑139 (MQ=255)                                                         tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  2:1407958/83‑2 (MQ=255)                                                         tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  >  1:1407958/1‑82 (MQ=255)                                                            taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCg                                                                        <  1:1554890/137‑1 (MQ=255)                                                            taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCg                                                                        >  2:1554890/1‑137 (MQ=255)                                                            taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                      >  1:287187/1‑139 (MQ=255)                                                              cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    <  2:507346/139‑1 (MQ=255)                                                                 ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  >  2:1107389/1‑74 (MQ=255)                                                                 ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  1:1107389/75‑2 (MQ=255)                                                                 ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGa                                                                                                  <  2:1275658/106‑1 (MQ=255)                                                                 ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGa                                                                                                  >  1:1275658/1‑106 (MQ=255)                                                                 ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:201060/1‑139 (MQ=255)                                                                   ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           >  1:1360830/1‑111 (MQ=255)                                                                   ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  2:1360830/111‑1 (MQ=255)                                                                      atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  1:1123096/108‑1 (MQ=255)                                                                      atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           >  2:1123096/1‑108 (MQ=255)                                                                      atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                            >  2:785727/1‑139 (MQ=255)                                                                           aCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAg                                                       >  1:881650/1‑139 (MQ=255)                                                                             cTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTc                                                                                                    >  2:1402582/1‑92 (MQ=255)                                                                             cTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTc                                                                                                    <  1:1402582/92‑1 (MQ=255)                                                                              tGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  >  1:978197/1‑61 (MQ=255)                                                                              tGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  2:978197/62‑2 (MQ=255)                                                                               ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCATCGttttta                                                                                                                                  <  1:1127073/61‑2 (MQ=255)                                                                               ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  >  2:1127073/1‑60 (MQ=255)                                                                                gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         >  2:462815/1‑100 (MQ=255)                                                                                gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:462815/100‑1 (MQ=255)                                                                                gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  2:287187/139‑1 (MQ=255)                                                                                gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  2:881650/139‑1 (MQ=255)                                                                                gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  2:995330/139‑1 (MQ=255)                                                                                 tttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  >  1:365128/1‑58 (MQ=255)                                                                                 tttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  2:365128/59‑2 (MQ=255)                                                                                 tttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  2:1061281/59‑2 (MQ=255)                                                                                 tttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGAGttttta                                                                                                                                  >  1:1061281/1‑58 (MQ=255)                                                                                   tATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                >  1:351560/1‑56 (MQ=255)                                                                                   tATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                <  2:351560/59‑4 (MQ=255)                                                                                     ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACa                                                        <  2:198288/128‑1 (MQ=255)                                                                                     ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACa                                                        >  1:198288/1‑128 (MQ=255)                                                                                     ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:394674/1‑139 (MQ=255)                                                                                     ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:770425/1‑139 (MQ=255)                                                                                     ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:1598548/1‑139 (MQ=255)                                                                                       gACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  >  1:1458827/1‑52 (MQ=255)                                                                                       gACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  2:1458827/53‑2 (MQ=255)                                                                                          cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                >  1:1608543/1‑49 (MQ=255)                                                                                          cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                <  2:1608543/52‑4 (MQ=255)                                                                                          cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:1688427/1‑139 (MQ=255)                                                                                          cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  1:783581/1‑139 (MQ=255)                                                                                           ggCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  <  1:652632/49‑2 (MQ=255)                                                                                           ggCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta                                                                                                                                  >  2:652632/1‑48 (MQ=255)                                                                                             cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                >  2:1232504/1‑46 (MQ=255)                                                                                             cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                <  1:1232504/49‑4 (MQ=255)                                                                                               tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                >  1:1342554/1‑44 (MQ=255)                                                                                               tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg                                                                                                                                <  2:1342554/47‑4 (MQ=255)                                                                                                cTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTcc                                  <  2:770425/139‑1 (MQ=255)                                                                                                  gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  1:1130413/1‑85 (MQ=255)                                                                                                  gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      <  2:1130413/85‑1 (MQ=255)                                                                                                    aCGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                              <  2:783581/139‑1 (MQ=255)                                                                                                      ggCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                            <  1:785727/139‑1 (MQ=255)                                                                                                       gCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  1:1546666/1‑86 (MQ=255)                                                                                                       gCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                <  2:1546666/86‑1 (MQ=255)                                                                                                                  aGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGa                >  1:462515/1‑139 (MQ=255)                                                                                                                      tGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                   >  2:1481288/1‑52 (MQ=255)                                                                                                                      tGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                   <  1:1481288/52‑1 (MQ=255)                                                                                                                        gTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  1:647842/99‑1 (MQ=255)                                                                                                                        gTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  >  2:647842/1‑99 (MQ=255)                                                                                                                         tGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  >  1:1721531/1‑98 (MQ=255)                                                                                                                         tGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  2:1721531/98‑1 (MQ=255)                                                                                                                          gAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt        <  1:1142456/139‑1 (MQ=255)                                                                                                                          gAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt        <  1:1688427/139‑1 (MQ=255)                                                                                                                           aaTCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACa                                                        <  2:1682415/90‑1 (MQ=255)                                                                                                                           aaTCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACa                                                        >  1:1682415/1‑90 (MQ=255)                                                                                                                            aTCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  2:669939/54‑1 (MQ=255)                                                                                                                            aTCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           >  1:669939/1‑54 (MQ=255)                                                                                                                                ccAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcatc  >  2:327186/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                      AGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NC_000913/1868884‑1869150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑