Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 183,607 Δ1 bp coding (1145/1158 nt) cdaR → carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913183,6070A.98.2% 214.7 / ‑3.0 55coding (1145/1158 nt)cdaRcarbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (30/24);  minor base T (1/0);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAACGTCAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCT  >  NC_000913/183476‑183739
                                                                                                                                   |                                                                                                                                    
ggCAACGTCAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGc                                                                                                                              >  2:641628/1‑139 (MQ=255)
   aaCGTCAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGt                                                                                                                           <  2:726533/139‑1 (MQ=255)
                            cGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggcgg                                                                                                  >  1:1524210/1‑139 (MQ=255)
                             gTAATACCCTGGAGTATCGACTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGt                                                                                                                           <  2:91468/113‑1 (MQ=255)
                             gTAATACCCTGGAGTATCGACTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGt                                                                                                                           >  1:91468/1‑113 (MQ=255)
                              tAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                <  1:1522853/139‑1 (MQ=255)
                                         aGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTc                                                                                     >  1:1333986/1‑139 (MQ=255)
                                             tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                     >  2:971854/1‑103 (MQ=255)
                                             tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                     <  1:971854/103‑1 (MQ=255)
                                             tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                >  2:589915/1‑124 (MQ=255)
                                             tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                <  1:589915/124‑1 (MQ=255)
                                             tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                 <  2:1323836/139‑1 (MQ=255)
                                             tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                 >  1:1323836/1‑139 (MQ=255)
                                                      tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                        <  2:1333986/139‑1 (MQ=255)
                                                                gAACTGACCGGGCTTGATTTGGGCATTTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAggg                                                              >  1:985131/1‑139 (MQ=255)
                                                                gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                          <  1:879762/127‑1 (MQ=255)
                                                                gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                          >  2:879762/1‑127 (MQ=255)
                                                                gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAggg                                                              >  2:1057559/1‑139 (MQ=255)
                                                                   cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGaa                                                           >  1:1683013/1‑139 (MQ=255)
                                                                   cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTACGTGGCGTTACAACTGTTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGaa                                                           >  1:1103564/1‑139 (MQ=255)
                                                                      aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                          >  1:582193/1‑121 (MQ=255)
                                                                      aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                        >  1:717538/1‑139 (MQ=255)
                                                                      aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                        >  1:828058/1‑139 (MQ=255)
                                                                      aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                        >  1:1109846/1‑139 (MQ=255)
                                                                      aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                        <  2:828058/139‑1 (MQ=255)
                                                                      aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                        >  2:687978/1‑139 (MQ=255)
                                                                      aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                        >  1:1400191/1‑139 (MQ=255)
                                                                      aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCCGTAAGTTATGCGTTAAGGTTGAGGCGGTGCTGGCTCATTCCCCTCAGCCCg                                                                          <  2:582193/121‑1 (MQ=255)
                                                                                aTTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAAc                                              >  2:1308032/1‑139 (MQ=255)
                                                                                   tGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                           <  2:1109846/139‑1 (MQ=255)
                                                                                     ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                         >  1:7927/1‑139 (MQ=255)
                                                                                     ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCa                                         >  1:1518517/1‑139 (MQ=255)
                                                                                        aaTTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                      <  2:1518517/139‑1 (MQ=255)
                                                                                             tgatgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGa                                 <  2:985131/139‑1 (MQ=255)
                                                                                               atgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGatt                               >  1:1552595/1‑139 (MQ=255)
                                                                                                   cAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTAttt                           <  1:1308032/139‑1 (MQ=255)
                                                                                                      gTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAgggg                                                             >  2:129041/1‑102 (MQ=255)
                                                                                                      gTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAgggg                                                             <  1:129041/102‑1 (MQ=255)
                                                                                                      gTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAcg                        >  2:99954/1‑139 (MQ=255)
                                                                                                            gctgTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                 >  2:1276413/1‑76 (MQ=255)
                                                                                                            gctgTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                 <  1:1276413/76‑1 (MQ=255)
                                                                                                            gctgTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAg                  <  1:99954/139‑1 (MQ=255)
                                                                                                             ctgTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGt                 <  1:1057559/139‑1 (MQ=255)
                                                                                                                  tgtgGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                          >  2:806780/1‑77 (MQ=255)
                                                                                                                  tgtgGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                          <  1:806780/77‑1 (MQ=255)
                                                                                                                  tgtgGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                      <  1:969492/113‑1 (MQ=255)
                                                                                                                  tgtgGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                      >  2:969492/1‑113 (MQ=255)
                                                                                                                  tgtgGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAcgcg                      <  2:1570454/129‑1 (MQ=255)
                                                                                                                  tgtgGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAcgcg                      >  1:1570454/1‑129 (MQ=255)
                                                                                                                     ggCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                <  2:1232241/52‑1 (MQ=255)
                                                                                                                     ggCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGgcggcg                                                                                                >  1:1232241/1‑52 (MQ=255)
                                                                                                                      gCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGa        <  2:1552595/139‑1 (MQ=255)
                                                                                                                       cGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt       <  2:1400191/139‑1 (MQ=255)
                                                                                                                       cGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt       >  2:980707/1‑139 (MQ=255)
                                                                                                                            cAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCt  >  2:871820/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                    
GGCAACGTCAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCT  >  NC_000913/183476‑183739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: