Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,214,339	(C)6→5	coding (306/1686 nt)	yehU ←	inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,214,334	0	C	.	100.0%	185.3 / NA	46	coding (311/1686 nt)	yehU	inner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (24/22);  total (24/22)

GACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAA  >  NC_000913/2214198‑2214461                                                                                                                                         |                                                                                                                                gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                               <  1:1286566/139‑1 (MQ=255)     aaCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                               >  1:61669/1‑135 (MQ=255)     aaCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                               <  2:61669/135‑1 (MQ=255)           aCGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAata                                                                                                                    <  1:1562935/139‑1 (MQ=255)               cACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGa                                                                                                                >  2:1130602/1‑139 (MQ=255)                              ggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTc                                                                                                 >  1:860062/1‑139 (MQ=255)                               gTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatc                                                                                                                          <  2:228842/114‑4 (MQ=255)                               gTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccatc                                                                                                                          >  1:228842/1‑111 (MQ=255)                                               gTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCAcc                                                                                <  1:937858/139‑1 (MQ=255)                                                    cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgacga                                                                           <  2:608076/139‑1 (MQ=255)                                                     ccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAatat                                                                                                                   >  2:1488217/1‑98 (MQ=255)                                                     ccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAatat                                                                                                                   <  1:1488217/98‑1 (MQ=255)                                                        cggcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                               >  1:765377/1‑84 (MQ=255)                                                        cggcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                               <  2:765377/84‑1 (MQ=255)                                                          gcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                         >  2:751707/1‑119 (MQ=255)                                                          gcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                         <  1:751707/119‑1 (MQ=255)                                                            ggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggaccg                                                                   <  1:1130602/139‑1 (MQ=255)                                                                     tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                          <  2:860062/139‑1 (MQ=255)                                                                        tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAata                                                                                                                    >  1:398813/1‑78 (MQ=255)                                                                        tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAata                                                                                                                    <  2:398813/78‑1 (MQ=255)                                                                          tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                     >  1:1291447/1‑139 (MQ=255)                                                                           gtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                               >  2:403523/1‑65 (MQ=255)                                                                           gtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                               <  1:403523/65‑1 (MQ=255)                                                                                 ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccccca                                                                                                                            <  1:397444/62‑2 (MQ=255)                                                                                 ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccccca                                                                                                                            >  2:397444/1‑61 (MQ=255)                                                                                 ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                               >  2:1070480/1‑59 (MQ=255)                                                                                 ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                               <  1:1070480/59‑1 (MQ=255)                                                                                 ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgga                                                                      >  1:362440/1‑115 (MQ=255)                                                                                 ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgga                                                                      <  2:362440/115‑1 (MQ=255)                                                                                  gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                          >  1:1235481/1‑126 (MQ=255)                                                                                  gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                          <  2:1235481/126‑1 (MQ=255)                                                                                      ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGt                                                                                                  <  1:581369/82‑1 (MQ=255)                                                                                      ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGt                                                                                                  >  2:581369/1‑82 (MQ=255)                                                                                      ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                         >  1:762949/1‑139 (MQ=255)                                                                                       cGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggacc                                                                    >  1:1279131/1‑111 (MQ=255)                                                                                       cGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggacc                                                                    <  2:1279131/111‑1 (MQ=255)                                                                                        gAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTa                                       >  1:1367627/1‑139 (MQ=255)                                                                                                   cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTa                            <  2:762949/139‑1 (MQ=255)                                                                                                    aaCGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAt                           >  1:605441/1‑139 (MQ=255)                                                                                                         tGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                      >  2:853509/1‑139 (MQ=255)                                                                                                         tGGTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                      >  1:1558577/1‑139 (MQ=255)                                                                                                           gTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCAcc                                                                                <  2:193886/79‑1 (MQ=255)                                                                                                           gTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCAcc                                                                                >  1:193886/1‑79 (MQ=255)                                                                                                           gTCGAGATCATGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAAt                    >  2:438755/1‑139 (MQ=255)                                                                                                                    aTGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGt                                                             >  2:670584/1‑89 (MQ=255)                                                                                                                    aTGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGt                                                             <  1:670584/89‑1 (MQ=255)                                                                                                                     tGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                  >  2:1476203/1‑115 (MQ=255)                                                                                                                     tGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                  <  1:1476203/115‑1 (MQ=255)                                                                                                                     tGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa          >  2:1213261/1‑139 (MQ=255)                                                                                                                     tGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa          >  1:861292/1‑139 (MQ=255)                                                                                                                     tGCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa          <  2:861292/139‑1 (MQ=255)                                                                                                                      gCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                          >  2:1520366/1‑90 (MQ=255)                                                                                                                      gCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                          <  1:1520366/90‑1 (MQ=255)                                                                                                                      gCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                  <  2:661044/114‑1 (MQ=255)                                                                                                                      gCAACTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                  >  1:661044/1‑114 (MQ=255)                                                                                                                          cTTAACGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg     <  1:853509/139‑1 (MQ=255)                                                                                                                             aaCGCGGTCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCaa  <  1:1213261/139‑1 (MQ=255)                                                                                                                                    tCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg     >  2:1211826/1‑129 (MQ=255)                                                                                                                                    tCATG‑CCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg     <  1:1211826/129‑1 (MQ=255)                                                                                                                                         |                                                                                                                                GACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAA  >  NC_000913/2214198‑2214461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑