Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,447,869 Δ1 bp coding (3758/3780 nt) tamB → translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,447,8670T.100.0% 240.2 / NA 59coding (3756/3780 nt)tamBtranslocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (30/29);  total (30/29)

TGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTG  >  NC_000913/4447743‑4447998
                                                                                                                            |                                                                                                                                   
tGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTc                                                                                                                      >  1:244675/1‑139 (MQ=255)
     ggTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGtt                                                                                                                 >  2:1734092/1‑139 (MQ=255)
       tCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAt                                                                                                                                     >  2:1630785/1‑118 (MQ=255)
       tCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAt                                                                                                                                     <  1:1630785/118‑1 (MQ=255)
       tCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGtt                                                                                                                       >  2:492476/1‑131 (MQ=255)
       tCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGtt                                                                                                                       <  1:492476/131‑1 (MQ=255)
       tCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGtttt                                                                                                               >  1:269565/1‑139 (MQ=255)
         tGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTaa                                                                                                             >  2:808918/1‑138 (MQ=255)
           cAAGTTAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCGGGAAGCCGTGTCTGGTGTAGACCAGGAACTGGATTGCTCTATCAGTTCGAGTTTTAACa                                                                                                           <  2:244675/139‑1 (MQ=255)
                     aCGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                 <  1:808918/139‑1 (MQ=255)
                                          ctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                            <  2:772484/139‑1 (MQ=255)
                                          ctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGcc                                                                            <  2:1289722/139‑1 (MQ=255)
                                             taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTaaca                                                                                                           >  1:915176/1‑102 (MQ=255)
                                             taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACa                                                                                                           <  2:915176/105‑1 (MQ=255)
                                               gCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaa                                                                       <  2:269565/139‑1 (MQ=255)
                                                 aaCACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGCATATTTGt                                                                                             <  2:102026/115‑1 (MQ=255)
                                                 aaCACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGt                                                                                             >  1:102026/1‑115 (MQ=255)
                                                     cTCACGTTACGTTATCGCCTGATGCGTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTaa                                                                                                             <  2:894133/95‑2 (MQ=255)
                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTaa                                                                                                             >  1:894133/1‑94 (MQ=255)
                                                      tCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCa                                                                >  2:671221/1‑139 (MQ=255)
                                                       cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                               <  1:671221/139‑1 (MQ=255)
                                                          gttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAg                                                            >  1:1695907/1‑139 (MQ=255)
                                                              cgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTaac                                                                                                            <  1:576323/87‑3 (MQ=255)
                                                              cgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTaac                                                                                                            >  2:576323/1‑85 (MQ=255)
                                                                      ccTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                 <  2:826232/90‑1 (MQ=255)
                                                                      ccTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                 >  1:826232/1‑90 (MQ=255)
                                                                         gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                             <  1:1734092/139‑1 (MQ=255)
                                                                          aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAAt                                                                                                         <  2:812173/78‑1 (MQ=255)
                                                                          aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAAt                                                                                                         >  1:812173/1‑78 (MQ=255)
                                                                          aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                           >  2:175319/1‑124 (MQ=255)
                                                                          aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                           <  1:175319/124‑1 (MQ=255)
                                                                          aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAAt                                            >  1:885244/1‑139 (MQ=255)
                                                                           tGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATg                                           <  2:1695907/139‑1 (MQ=255)
                                                                                 aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                 <  1:1526992/79‑1 (MQ=255)
                                                                                 aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAatat                                                                                                 >  2:1526992/1‑79 (MQ=255)
                                                                                      tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGtt                                                                                                                       >  1:1703028/1‑52 (MQ=255)
                                                                                      tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGtt                                                                                                                       <  2:1703028/52‑1 (MQ=255)
                                                                                      tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                   <  2:630403/120‑1 (MQ=255)
                                                                                      tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                   >  1:630403/1‑120 (MQ=255)
                                                                                      tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTg                                >  1:1602924/1‑139 (MQ=255)
                                                                                            gAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttgct                                                                                                                                 <  1:1531519/37‑4 (MQ=255)
                                                                                            gAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttgct                                                                                                                                 >  2:1531519/1‑34 (MQ=255)
                                                                                             aaGCCGTTTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTa                                                                                <  2:1239018/84‑1 (MQ=255)
                                                                                             aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTa                                                                                >  1:1239018/1‑84 (MQ=255)
                                                                                             aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                               >  2:1241191/1‑101 (MQ=255)
                                                                                             aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                               <  1:1241191/101‑1 (MQ=255)
                                                                                             aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCa                                                         <  2:170945/107‑1 (MQ=255)
                                                                                             aaGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCa                                                         >  1:170945/1‑107 (MQ=255)
                                                                                              aGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCg                                                                                                      <  2:1152601/61‑1 (MQ=255)
                                                                                              aGCCGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCg                                                                                                      >  1:1152601/1‑61 (MQ=255)
                                                                                                ccGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                             <  1:742645/132‑1 (MQ=255)
                                                                                                ccGTGTCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                             >  2:742645/1‑132 (MQ=255)
                                                                                                  gtgtCTGGTGTAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGt                    >  2:1344123/1‑139 (MQ=255)
                                                                                                           gtAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                         <  1:239476/109‑1 (MQ=255)
                                                                                                           gtAGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                         >  2:239476/1‑109 (MQ=255)
                                                                                                             aGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                               <  1:53346/85‑1 (MQ=255)
                                                                                                             aGACCAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                               >  2:53346/1‑85 (MQ=255)
                                                                                                                 cAGGCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg     <  1:1344123/139‑1 (MQ=255)
                                                                                                                   ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTa                                                                                <  2:296906/62‑1 (MQ=255)
                                                                                                                   ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTa                                                                                >  1:296906/1‑62 (MQ=255)
                                                                                                                   ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGa               <  2:724707/127‑1 (MQ=255)
                                                                                                                   ggCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGa               >  1:724707/1‑127 (MQ=255)
                                                                                                                    gCACTGGATTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg  >  1:483222/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                                   
TGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTG  >  NC_000913/4447743‑4447998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: