Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 1,053,516 | (G)6→7 | coding (2663/2745 nt) | torS ← | hybrid sensory histidine kinase in two‑component regulatory system with TorR |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,053,510 | 1 | . | G | 91.9% | 205.7 / 9.0 | 62 | coding (2669/2745 nt) | torS | hybrid sensory histidine kinase in two‑component regulatory system with TorR |
Reads supporting (aligned to +/- strand): ref base . (3/2); new base G (31/26); total (34/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 |
CCGTTAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGG > NC_000913/1053375‑1053642 | ccGTTAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑ggg > 2:79471/1‑139 (MQ=255) tAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑ggggggg < 1:555939/139‑2 (MQ=255) taCCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATg < 1:287181/139‑1 (MQ=255) aTGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGgc > 2:1549270/1‑135 (MQ=255) aTGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGgc < 1:1549270/135‑1 (MQ=255) gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGgctgctgc > 2:313793/1‑139 (MQ=255) gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGgctgc < 1:742974/136‑1 (MQ=255) gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGgctgc > 2:742974/1‑136 (MQ=255) ttCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGtt < 1:313793/139‑1 (MQ=255) gttCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCAt > 2:246520/1‑139 (MQ=255) tCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAg > 2:1485851/1‑139 (MQ=255) tCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAg > 2:138353/1‑139 (MQ=255) tAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAgctgg < 1:571205/139‑1 (MQ=255) gCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCtggctggc > 2:1334679/1‑139 (MQ=255) ttGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGc < 2:45525/139‑1 (MQ=255) ttGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGc < 1:1204021/139‑1 (MQ=255) aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTgcg > 2:274060/1‑115 (MQ=255) aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTgcg < 1:274060/115‑1 (MQ=255) aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAgctggc < 2:276030/125‑1 (MQ=255) aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAgctggc > 1:276030/1‑125 (MQ=255) aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCAtt > 2:314607/1‑139 (MQ=255) aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑gg < 1:1613169/81‑1 (MQ=255) aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑gg > 2:1613169/1‑81 (MQ=255) aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑g < 1:274852/80‑1 (MQ=255) aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑g > 2:274852/1‑80 (MQ=255) aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGgctgctgct > 1:1546291/1‑103 (MQ=255) aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGgctgctgct < 2:1546291/103‑1 (MQ=255) aaTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg > 2:1558094/1‑139 (MQ=255) aaTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg < 1:1042646/139‑1 (MQ=255) ttCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGa > 2:1420863/1‑119 (MQ=255) ttCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGa < 1:1420863/119‑1 (MQ=255) tATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa > 2:1293570/1‑129 (MQ=255) tATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa < 1:1293570/129‑1 (MQ=255) tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAAc < 1:397803/128‑1 (MQ=255) tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAAc > 2:397803/1‑128 (MQ=255) tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCt > 2:1489908/1‑139 (MQ=255) gCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCt > 2:1296279/1‑139 (MQ=255) ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAg > 2:913346/1‑88 (MQ=255) ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAg < 1:913346/88‑1 (MQ=255) ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg < 2:639531/112‑1 (MQ=255) ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg > 1:639531/1‑112 (MQ=255) ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑ggggggg < 2:1115015/54‑2 (MQ=255) ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑ggggggg > 1:1115015/1‑53 (MQ=255) ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGAGGAAGGGGGGGCA‑GGTAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATg < 1:1296780/139‑1 (MQ=255) ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGAGGAAGGGGGGGCA‑GGTAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATg > 2:1296780/1‑139 (MQ=255) tGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑g > 1:501400/1‑45 (MQ=255) tGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑g < 2:501400/45‑1 (MQ=255) cTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCgg < 1:138353/139‑1 (MQ=255) cTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCgg < 2:491476/139‑1 (MQ=255) ccAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCAc > 1:1631114/1‑139 (MQ=255) ccAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCAc > 1:1251833/1‑139 (MQ=255) agCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa < 2:1254595/110‑1 (MQ=255) agCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa > 1:1254595/1‑110 (MQ=255) gCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGcgcg > 2:138313/1‑127 (MQ=255) gCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGcgcg < 1:138313/127‑1 (MQ=255) cAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTAttt > 1:382204/1‑139 (MQ=255) cAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTAttt > 1:1376148/1‑139 (MQ=255) cAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGGAGGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGGGCAACTGCTTTTTAGCTGATGCGCGGCACGCTtttttt > 1:520256/1‑139 (MQ=255) gCGTGTTATTTCTTCCTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCt < 2:520256/139‑1 (MQ=255) gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGc > 1:1100259/1‑121 (MQ=255) gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGc < 2:1100259/121‑1 (MQ=255) gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCt < 2:382204/139‑1 (MQ=255) cGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCa < 2:437199/86‑1 (MQ=255) cGTGTAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCa > 1:437199/1‑86 (MQ=255) gtgtAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTAt > 1:1128794/1‑139 (MQ=255) tgtAATTTCTTCGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATc > 1:975438/1‑139 (MQ=255) cttcGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTgg > 1:1431657/1‑139 (MQ=255) tcGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGtt > 2:347419/1‑41 (MQ=38) tcGTGTGGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGtt < 1:347419/41‑1 (MQ=38) gtgGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCggg < 1:79471/139‑1 (MQ=255) tgGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTcc < 1:639644/67‑1 (MQ=255) tgGAAGGGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTcc > 2:639644/1‑67 (MQ=255) | CCGTTAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAA‑GGGGGGCA‑GATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGG > NC_000913/1053375‑1053642 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |