Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,053,516 (G)6→7 coding (2663/2745 nt) torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,053,5101.G91.9% 205.7 / 9.0 62coding (2669/2745 nt)torShybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand):  ref base . (3/2);  new base G (31/26);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01

CCGTTAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGG  >  NC_000913/1053375‑1053642
                                                                                                                                        |                                                                                                                                     
ccGTTAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAggg                                                                                                                                    >  2:79471/1‑139 (MQ=255)
    tAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAggggggg                                                                                                                                <  1:555939/139‑2 (MQ=255)
             taCCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATg                                                                                                                       <  1:287181/139‑1 (MQ=255)
                   aTGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgc                                                                                                                     >  2:1549270/1‑135 (MQ=255)
                   aTGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgc                                                                                                                     <  1:1549270/135‑1 (MQ=255)
                     gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgctgc                                                                                                               >  2:313793/1‑139 (MQ=255)
                     gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgc                                                                                                                  <  1:742974/136‑1 (MQ=255)
                     gCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGgctgc                                                                                                                  >  2:742974/1‑136 (MQ=255)
                            ttCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGtt                                                                                                        <  1:313793/139‑1 (MQ=255)
                                    gttCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCAt                                                                                                >  2:246520/1‑139 (MQ=255)
                                      tCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAg                                                                                              >  2:1485851/1‑139 (MQ=255)
                                      tCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAg                                                                                              >  2:138353/1‑139 (MQ=255)
                                         tAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgctgg                                                                                          <  1:571205/139‑1 (MQ=255)
                                               gCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCtggctggc                                                                                     >  2:1334679/1‑139 (MQ=255)
                                                      ttGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGc                                                                              <  2:45525/139‑1 (MQ=255)
                                                      ttGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGc                                                                              <  1:1204021/139‑1 (MQ=255)
                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTgcg                                                                                                   >  2:274060/1‑115 (MQ=255)
                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTgcg                                                                                                   <  1:274060/115‑1 (MQ=255)
                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgctggc                                                                                         <  2:276030/125‑1 (MQ=255)
                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAgctggc                                                                                         >  1:276030/1‑125 (MQ=255)
                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCAtt                                                                           >  2:314607/1‑139 (MQ=255)
                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAgg                                                                                                                                     <  1:1613169/81‑1 (MQ=255)
                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAgg                                                                                                                                     >  2:1613169/1‑81 (MQ=255)
                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAg                                                                                                                                      <  1:274852/80‑1 (MQ=255)
                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAg                                                                                                                                      >  2:274852/1‑80 (MQ=255)
                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGgctgctgct                                                                                                              >  1:1546291/1‑103 (MQ=255)
                                                         aTTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGgctgctgct                                                                                                              <  2:1546291/103‑1 (MQ=255)
                                                              aaTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg                                                                      >  2:1558094/1‑139 (MQ=255)
                                                              aaTCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg                                                                      <  1:1042646/139‑1 (MQ=255)
                                                                     ttCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGa                                                                                   >  2:1420863/1‑119 (MQ=255)
                                                                     ttCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGa                                                                                   <  1:1420863/119‑1 (MQ=255)
                                                                                  tATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa                                                            >  2:1293570/1‑129 (MQ=255)
                                                                                  tATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa                                                            <  1:1293570/129‑1 (MQ=255)
                                                                                    tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAAc                                                           <  1:397803/128‑1 (MQ=255)
                                                                                    tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAAc                                                           >  2:397803/1‑128 (MQ=255)
                                                                                    tGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCt                                               >  2:1489908/1‑139 (MQ=255)
                                                                                     gCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCt                                               >  2:1296279/1‑139 (MQ=255)
                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAg                                                                                              >  2:913346/1‑88 (MQ=255)
                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAg                                                                                              <  1:913346/88‑1 (MQ=255)
                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg                                                                      <  2:639531/112‑1 (MQ=255)
                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAg                                                                      >  1:639531/1‑112 (MQ=255)
                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAggggggg                                                                                                                                <  2:1115015/54‑2 (MQ=255)
                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAggggggg                                                                                                                                >  1:1115015/1‑53 (MQ=255)
                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGAGGAAGGGGGGGCAGGTAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATg                                           <  1:1296780/139‑1 (MQ=255)
                                                                                         ccATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGAGGAAGGGGGGGCAGGTAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATg                                           >  2:1296780/1‑139 (MQ=255)
                                                                                            tGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAg                                                                                                                                      >  1:501400/1‑45 (MQ=255)
                                                                                            tGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAg                                                                                                                                      <  2:501400/45‑1 (MQ=255)
                                                                                              cTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCgg                                      <  1:138353/139‑1 (MQ=255)
                                                                                              cTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCgg                                      <  2:491476/139‑1 (MQ=255)
                                                                                                 ccAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCAc                                   >  1:1631114/1‑139 (MQ=255)
                                                                                                 ccAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCAc                                   >  1:1251833/1‑139 (MQ=255)
                                                                                                     agCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa                                                            <  2:1254595/110‑1 (MQ=255)
                                                                                                     agCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCaa                                                            >  1:1254595/1‑110 (MQ=255)
                                                                                                         gCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGcgcg                                       >  2:138313/1‑127 (MQ=255)
                                                                                                         gCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGcgcg                                       <  1:138313/127‑1 (MQ=255)
                                                                                                          cAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTAttt                          >  1:382204/1‑139 (MQ=255)
                                                                                                          cAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTAttt                          >  1:1376148/1‑139 (MQ=255)
                                                                                                          cAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGGAGGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGGGCAACTGCTTTTTAGCTGATGCGCGGCACGCTtttttt                          >  1:520256/1‑139 (MQ=255)
                                                                                                                 gCGTGTTATTTCTTCCTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCt                   <  2:520256/139‑1 (MQ=255)
                                                                                                                 gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGc                                     >  1:1100259/1‑121 (MQ=255)
                                                                                                                 gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGc                                     <  2:1100259/121‑1 (MQ=255)
                                                                                                                 gCGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCt                   <  2:382204/139‑1 (MQ=255)
                                                                                                                  cGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCa                                                                       <  2:437199/86‑1 (MQ=255)
                                                                                                                  cGTGTAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCa                                                                       >  1:437199/1‑86 (MQ=255)
                                                                                                                   gtgtAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTAt                 >  1:1128794/1‑139 (MQ=255)
                                                                                                                    tgtAATTTCTTCGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATc                >  1:975438/1‑139 (MQ=255)
                                                                                                                            cttcGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTgg        >  1:1431657/1‑139 (MQ=255)
                                                                                                                              tcGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGtt                                                                                                        >  2:347419/1‑41 (MQ=38)
                                                                                                                              tcGTGTGGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGtt                                                                                                        <  1:347419/41‑1 (MQ=38)
                                                                                                                                  gtgGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCggg  <  1:79471/139‑1 (MQ=255)
                                                                                                                                   tgGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTcc                                                                         <  1:639644/67‑1 (MQ=255)
                                                                                                                                   tgGAAGGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTcc                                                                         >  2:639644/1‑67 (MQ=255)
                                                                                                                                        |                                                                                                                                     
CCGTTAAGTTGTATACCAAATGCCACTATTCTAGTTGTTCTTAACTGGCTGATATTGATTCAAATCGCGTTCAGGTCTTTCTTATGCAACCATGCTTCCAGAGCGGCAACACTGCGTGTAATTTCTTCGTGTGGAAGGGGGGCAGATAATGGCTGCTGCTCCAGTTGTGCGCATAGCTGGCTGGCGATATGCATTCCCAGACTCGAGCAACTGCTTTTTAGCTGATGCGCGGCACGCTTTATTTTCTCGCTATCCTGACTGGCGCGGG  >  NC_000913/1053375‑1053642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: