Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,767,445 (C)7→8 intergenic (+90/‑265) rnlB → / → yfjP CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,4381.C88.9% 106.9 / 5.9 36intergenic (+83/‑272)rnlB/yfjPCP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand):  ref base . (4/0);  new base C (13/19);  total (17/19)
Fisher's exact test for biased strand distribution p-value = 4.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGT  >  NC_000913/2767301‑2767565
                                                                                                                                           |                                                                                                                                  
aGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑c                                                                                                                                  >  2:677567/1‑139 (MQ=255)
aGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑c                                                                                                                                  <  1:677567/139‑1 (MQ=255)
   ctctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑cccc                                                                                                                              <  2:584638/139‑1 (MQ=255)
   ctctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑cccc                                                                                                                              <  1:72601/139‑1 (MQ=255)
    tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑ccccc                                                                                                                            <  1:1515758/139‑1 (MQ=255)
    tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑ccccc                                                                                                                            <  1:73658/139‑1 (MQ=255)
       gTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑cccccccc                                                                                                                         <  1:1118808/139‑2 (MQ=255)
            gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                     <  2:38498/139‑1 (MQ=255)
            gTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                     <  1:444973/139‑1 (MQ=255)
             ttccccGATGGTGTACGCCAAGTCCTGTCAAAGGATTTTTGAAACCCTTCTTTCTAAACGAACCAGTAAAGAATTCGCCCTTTTGGCATGATATTAACCTGTCCAATATGATCCAATAGCCTTAA‑‑ccccccccctcaca                                                                                                                   <  1:486274/136‑8 (MQ=255)
               tcCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACg                                                                                                                  >  1:985656/1‑139 (MQ=255)
                                  gTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAA‑‑CCCCCCCTCACAACGTCCTGCATAAGGAacacat                                                                                               >  2:210695/1‑137 (MQ=255)
                                        tCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTcc                                                                                          >  2:1410395/1‑139 (MQ=255)
                                        tCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                         <  2:985656/139‑1 (MQ=255)
                                        tCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                         <  2:811888/139‑1 (MQ=255)
                                         cAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                         <  1:1410395/139‑1 (MQ=255)
                                             ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                     >  1:578264/1‑106 (MQ=255)
                                             ggATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTcaca                                                                                                                     <  2:578264/106‑1 (MQ=255)
                                                  tttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑cccccccc                                                                                                                         >  1:600662/1‑95 (MQ=255)
                                                  tttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑cccccccc                                                                                                                         <  2:600662/96‑2 (MQ=255)
                                                  tttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑cccc                                                                                                                              >  2:909279/1‑92 (MQ=255)
                                                  tttGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑cccc                                                                                                                              <  1:909279/92‑1 (MQ=255)
                                                              ctttctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                   <  2:1148984/107‑1 (MQ=255)
                                                              ctttctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑c                                                                                                                                  <  1:309820/77‑1 (MQ=255)
                                                              ctttctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑c                                                                                                                                  >  2:309820/1‑77 (MQ=255)
                                                              ctttctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑CCCACCCCTCACAACGTCCTGCATAGGGAac                                                                                                   >  1:1148984/1‑107 (MQ=255)
                                                                    aaaCGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTcccccc                                                             >  2:1018128/1‑139 (MQ=255)
                                                                    aaaCGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑CCCCACCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTcccccc                                                             >  2:587810/1‑139 (MQ=255)
                                                                         aaCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTa                                                        <  1:1426516/139‑1 (MQ=255)
                                                                          aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCCCAACGTCCTGCATAGGGAac                                                                                                   >  1:1457894/1‑95 (MQ=255)
                                                                          aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAac                                                                                                   <  2:1457894/95‑1 (MQ=255)
                                                                          aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                         <  2:837285/105‑1 (MQ=255)
                                                                          aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                         >  1:837285/1‑105 (MQ=255)
                                                                              gTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGATTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACtt                                                   <  1:453257/139‑1 (MQ=255)
                                                                                    aaTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                >  1:148505/1‑72 (MQ=255)
                                                                                    aaTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTc                                                                                                                <  2:148505/72‑1 (MQ=255)
                                                                                        cGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc                                                                >  2:699974/1‑116 (MQ=255)
                                                                                        cGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTccc                                                                <  1:699974/116‑1 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACg                                                                                                <  2:786757/83‑1 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACg                                                                                                >  1:786757/1‑83 (MQ=255)
                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAAAACAATATCCCCTGTGCACTCACGACTAAATTTCCCCCCCCTTAAACTTCTCattaaaat                                        >  2:453257/1‑136 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACTTCCTGCATAGGGAACACGTTCTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACt                                                    >  1:1347993/1‑102 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGtttt                                                                                            <  1:1457644/62‑1 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGtttt                                                                                            >  2:1457644/1‑62 (MQ=255)
                                                                                                                  tCCAATATGATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACt                                                    <  2:1347993/102‑1 (MQ=255)
                                                                                                                          gATCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc       <  1:559018/139‑1 (MQ=255)
                                                                                                                           aTCAAATAGCATTAACCCCCCCCTCACAACGTCCTGCATAGCGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTcc      <  2:1344534/139‑1 (MQ=255)
                                                                                                                               aaTAGCATTAA‑‑CCCCACCCTCACAACGTCCTGCATAGGGAACACGTTTTACCCTGTGCACCCACGACTAAAATTCCCCCCCTTACAACTTCTCAATAATGTCACCTATTTTCTGGAAATCCTATGATGCCTTTCCCGGt  >  1:1344534/1‑139 (MQ=255)
                                                                                                                                  aGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc                                                                                <  1:1532526/58‑1 (MQ=255)
                                                                                                                                  aGCATTAACCCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCAcc                                                                                >  2:1532526/1‑58 (MQ=255)
                                                                                                                                           |                                                                                                                                  
AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGT  >  NC_000913/2767301‑2767565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: