Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,664,363 (G)6→7 coding (256/729 nt) gadW ← transcriptional activator of gadA and gadBC, repressor of gadX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,664,3571.G100.0% 417.4 / NA 113M88L (ATG→CTG) gadWtranscriptional activator of gadA and gadBC, repressor of gadX
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (58/55);  total (58/55)

Only 100 of 134 total aligned reads displayed.

ATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACA  >  NC_000913/3664223‑3664489
                                                                                                                                       |                                                                                                                                    
aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgggg                                                                                                                                  >  1:1155901/1‑139 (MQ=255)
aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgggg                                                                                                                                  <  2:1498533/139‑1 (MQ=255)
      aaGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgggggggtga                                                                                                                            <  1:1420910/139‑5 (MQ=255)
          aaGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTgg                                                                                                                         <  2:743239/139‑1 (MQ=255)
           aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgg                                                                                                                                    <  1:1004510/126‑1 (MQ=255)
           aGGGGATCAGTGCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGAATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgg                                                                                                                                    >  2:1004510/1‑126 (MQ=255)
                aTCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGttaatt                                                                                                                   >  1:378749/1‑139 (MQ=255)
                   aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                       <  2:1213127/116‑1 (MQ=255)
                   aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                       <  1:1508501/116‑1 (MQ=255)
                   aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                       >  2:1508501/1‑116 (MQ=255)
                   aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                       >  1:1213127/1‑116 (MQ=255)
                     ttctttctTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACaa                                                                                                              <  2:1155901/139‑1 (MQ=255)
                        tttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgggggggtga                                                                                                                            >  1:790622/1‑117 (MQ=255)
                        tttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgggggggtga                                                                                                                            <  2:790622/121‑5 (MQ=255)
                         ttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACgg                                                                                                          >  1:747754/1‑139 (MQ=255)
                          tcttATGAGAAAACATCGATAAACAGGAGAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         <  1:355181/139‑1 (MQ=255)
                          tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         <  1:363699/139‑1 (MQ=255)
                             tATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCtt                                                                                                      >  1:1024427/1‑139 (MQ=255)
                             tATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCtt                                                                                                      <  2:378749/139‑1 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  1:846302/139‑1 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  1:846885/1‑139 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  2:1115949/1‑139 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  1:70597/1‑139 (MQ=255)
                                    aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:1725558/139‑1 (MQ=255)
                                    aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  2:846885/139‑1 (MQ=255)
                                    aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  2:48806/139‑1 (MQ=255)
                                        aTCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAAc                                                                                                            >  1:767378/1‑122 (MQ=255)
                                        aTCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  1:1330860/1‑135 (MQ=255)
                                        aTCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  2:1330860/135‑1 (MQ=255)
                                          cGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCtt                                                                                                      <  1:1641614/126‑1 (MQ=255)
                                            aTAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         >  1:164980/1‑121 (MQ=255)
                                            aTAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         <  2:164980/121‑1 (MQ=255)
                                                    gAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAAt                                                                                    >  1:1218782/1‑134 (MQ=255)
                                                     aaaGGTAGAGCATATTGCTCAGTATCGGTGTATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTg                                                                              <  2:747754/139‑1 (MQ=255)
                                                       aGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGt                                                                            <  2:1414931/139‑1 (MQ=255)
                                                         gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACt                                                                                                                           >  1:47668/1‑90 (MQ=255)
                                                         gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgggggggt                                                                                                                              >  1:468844/1‑84 (MQ=255)
                                                         gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgggggggt                                                                                                                              <  2:468844/86‑3 (MQ=255)
                                                            gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGt                                                                               >  2:1577073/1‑131 (MQ=255)
                                                            gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGt                                                                               <  1:1577073/131‑1 (MQ=255)
                                                            gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTg                                                                       >  2:600062/1‑139 (MQ=255)
                                                                 tatTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGaa                                                                  <  2:1158952/139‑1 (MQ=255)
                                                                  atTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                                 <  1:1115949/139‑1 (MQ=255)
                                                                   ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAata                                                                >  1:1268020/1‑139 (MQ=255)
                                                                   ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAata                                                                >  1:1268818/1‑139 (MQ=255)
                                                                      cTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGAc                                                                     >  2:876561/1‑131 (MQ=255)
                                                                         aGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         <  1:263603/92‑1 (MQ=255)
                                                                         aGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGt                                                                                                         >  2:263603/1‑92 (MQ=255)
                                                                         aGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgt                                                          >  2:508331/1‑139 (MQ=255)
                                                                           tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAata                                                                <  1:513013/131‑1 (MQ=255)
                                                                           tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                                 <  2:737864/130‑1 (MQ=255)
                                                                           tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                                 >  1:737864/1‑130 (MQ=255)
                                                                            aTCGGTGAACTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCCTGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaag                                                       <  2:1024427/139‑1 (MQ=255)
                                                                              cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGttaatt                                                                                                                   >  1:1309210/1‑77 (MQ=255)
                                                                              cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGttaatt                                                                                                                   <  2:1309210/77‑1 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACt                                                                                                                           >  1:1367788/1‑67 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACt                                                                                                                           <  2:1367788/67‑1 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                                 >  2:525815/1‑125 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                                 <  1:525815/125‑1 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgggggg                                                                                                                                <  1:685905/61‑1 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgggggg                                                                                                                                <  2:4319/61‑1 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATgggggg                                                                                                                                >  2:685905/1‑61 (MQ=255)
                                                                                   aaTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGc                                                <  2:70597/139‑1 (MQ=255)
                                                                                     tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACgg                                                                                                          >  1:803715/1‑79 (MQ=255)
                                                                                     tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACgg                                                                                                          <  2:803715/79‑1 (MQ=255)
                                                                                     tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  1:2683/1‑90 (MQ=255)
                                                                                     tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  2:2683/90‑1 (MQ=255)
                                                                                     tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGa                                                                   >  1:609394/1‑118 (MQ=255)
                                                                                     tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGa                                                                   <  2:609394/118‑1 (MQ=255)
                                                                                          cccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  2:1389033/1‑85 (MQ=255)
                                                                                          cccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  1:1389033/85‑1 (MQ=255)
                                                                                             ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAg                                      <  2:506220/139‑1 (MQ=255)
                                                                                             ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAg                                      <  1:600062/139‑1 (MQ=255)
                                                                                             ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAg                                      >  1:1405981/1‑139 (MQ=255)
                                                                                                  cATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCAt                                 <  2:78666/139‑1 (MQ=255)
                                                                                                   aTTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                              >  1:1529896/1‑125 (MQ=255)
                                                                                                   aTTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                                    >  2:200663/1‑135 (MQ=255)
                                                                                                   aTTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                                    <  1:200663/135‑1 (MQ=255)
                                                                                                         gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACaa                                                                                                              >  2:529554/1‑55 (MQ=255)
                                                                                                         gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACaa                                                                                                              <  1:529554/55‑1 (MQ=255)
                                                                                                         gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGt                                                                            <  1:1261638/89‑1 (MQ=255)
                                                                                                         gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGt                                                                            >  2:1261638/1‑89 (MQ=255)
                                                                                                         gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATc                          >  1:812683/1‑139 (MQ=255)
                                                                                                         gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATc                          >  2:1546699/1‑139 (MQ=255)
                                                                                                            aTCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    >  1:699722/1‑62 (MQ=255)
                                                                                                            aTCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                                    <  2:699722/62‑1 (MQ=255)
                                                                                                             tCAAAAATCGCCGTCACCAGCTGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgt                      >  1:442572/1‑139 (MQ=255)
                                                                                                             tCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACaa                                                                                                              <  2:1525753/51‑1 (MQ=255)
                                                                                                             tCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACaa                                                                                                              >  1:1525753/1‑51 (MQ=255)
                                                                                                             tCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGa                                                                   >  2:1715833/1‑94 (MQ=255)
                                                                                                             tCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGa                                                                   <  1:1715833/94‑1 (MQ=255)
                                                                                                             tCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgt                      <  2:605569/139‑1 (MQ=255)
                                                                                                             tCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAACTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgt                      >  2:1243308/1‑139 (MQ=255)
                                                                                                                   aTCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTcgc                >  2:1611451/1‑139 (MQ=255)
                                                                                                                       ccGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGAc                                                                     <  2:1259470/82‑1 (MQ=255)
                                                                                                                       ccGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                         <  2:314460/110‑1 (MQ=255)
                                                                                                                       ccGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGt                                         >  1:314460/1‑110 (MQ=255)
                                                                                                                                 cGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               <  2:57621/46‑1 (MQ=255)
                                                                                                                                 cGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                               >  1:57621/1‑46 (MQ=255)
                                                                                                                                 cGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACa  <  2:1405981/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
ATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTATCCGGTGAACA  >  NC_000913/3664223‑3664489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: