Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,897,050 +G coding (277/666 nt) yieH → phosphoenolpyruvate and 6‑phosphogluconate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,897,0461.G100.0% 346.2 / NA 95E91E (GAA→GAG) yieHphosphoenolpyruvate and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (49/46);  total (49/46)

Only 100 of 104 total aligned reads displayed.

TGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896917‑3897177
                                                                                                                                  |                                                                                                                                   
tgtAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTATCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑gggggctgg                                                                                                                            >  2:859612/1‑134 (MQ=255)
  aaaaaCTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAg                                                                                                                           >  2:1083350/2‑139 (MQ=255)
         gTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCt                                                                                                                    >  1:1250899/1‑139 (MQ=255)
          tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  1:1399952/1‑139 (MQ=255)
          tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  2:83459/1‑139 (MQ=255)
          tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  2:789448/1‑139 (MQ=255)
          tACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  1:279905/1‑139 (MQ=255)
            cGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                      >  2:632792/1‑118 (MQ=255)
            cGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                      <  1:632792/118‑1 (MQ=255)
                    tCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  1:1240179/1‑129 (MQ=255)
                    tCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   <  2:1240179/129‑1 (MQ=255)
                    tCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCa                                                                                                         >  1:882399/1‑139 (MQ=255)
                      gATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACt                                                                                                       <  1:1367970/139‑1 (MQ=255)
                         atTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTgcg                                                                                                    >  1:1077695/1‑139 (MQ=255)
                              ttCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCTGCGATCACTGCGCCAAt                                                                                               <  1:1083350/139‑1 (MQ=255)
                              ttCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               <  2:882399/139‑1 (MQ=255)
                               tCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTATCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtg                                                                                              >  1:1455919/1‑139 (MQ=255)
                                  cTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgt                                                                                           >  2:527977/1‑139 (MQ=255)
                                   ttGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          <  2:1399952/139‑1 (MQ=255)
                                    tGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtgt                                                                                         >  1:1244761/1‑139 (MQ=255)
                                    tGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtgt                                                                                         >  1:253893/1‑139 (MQ=255)
                                     gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt                                                                                             >  1:985623/1‑134 (MQ=255)
                                     gAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt                                                                                             <  2:985623/134‑1 (MQ=255)
                                      aaCATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTc                                                                                                                      >  2:390535/1‑108 (MQ=255)
                                      aaCATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTc                                                                                                                      <  1:390535/108‑1 (MQ=255)
                                           ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt                                                                                  >  1:693025/1‑139 (MQ=255)
                                           ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt                                                                                  >  1:1121383/1‑139 (MQ=255)
                                           ggTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCt                                                                                  >  1:549501/1‑139 (MQ=255)
                                             tgtTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTaa                                                                                >  1:14628/1‑139 (MQ=255)
                                               ttACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACg                                                                              >  2:1090855/1‑139 (MQ=255)
                                                tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  1:735666/1‑101 (MQ=255)
                                                tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   <  2:735666/101‑1 (MQ=255)
                                                tACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCG‑‑CCTGTCAGCGATCACTGCGCCAATGTGTGGGGTATCTAACGGcc                                                                           >  2:174719/1‑139 (MQ=255)
                                                 aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                            <  2:1121383/139‑1 (MQ=255)
                                                 aCGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGGCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGc                                                                            <  2:693025/139‑1 (MQ=255)
                                                  cGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               <  2:776606/119‑1 (MQ=255)
                                                  cGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAt                                                                                               >  1:776606/1‑119 (MQ=255)
                                                      aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCt                                                                                                                       >  2:1131516/1‑91 (MQ=255)
                                                      aGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTCTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCt                                                                                                                       <  1:1131516/91‑1 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                      <  2:1354702/75‑1 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                      >  1:1354702/1‑75 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAgc                                                                                                                          >  2:160820/1‑87 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAgc                                                                                                                          <  1:160820/87‑1 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGc                                                                                                              <  2:1345631/99‑1 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGc                                                                                                              >  1:1345631/1‑99 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 <  1:907894/112‑1 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCa                                                                                                 >  2:907894/1‑112 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAAt                                                                      >  1:1571718/1‑139 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAAt                                                                      >  2:719176/1‑139 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g                                                                                                                                    <  1:668615/76‑1 (MQ=255)
                                                       gCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑g                                                                                                                                    >  2:668615/1‑76 (MQ=255)
                                                             aCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa                                                                <  1:624821/139‑1 (MQ=255)
                                                             aCAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACaaa                                                                <  1:1090855/139‑1 (MQ=255)
                                                                gAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATg                                                             <  2:1244761/139‑1 (MQ=255)
                                                                 aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  1:1341314/1‑84 (MQ=255)
                                                                 aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   <  2:1341314/84‑1 (MQ=255)
                                                                 aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGAt                                                                                                           <  1:439442/92‑1 (MQ=255)
                                                                 aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGAt                                                                                                           <  1:931044/92‑1 (MQ=255)
                                                                 aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGAt                                                                                                           >  2:931044/1‑92 (MQ=255)
                                                                 aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGAt                                                                                                           >  2:439442/1‑92 (MQ=255)
                                                                 aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  <  2:1541152/101‑1 (MQ=255)
                                                                 aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  >  1:1541152/1‑101 (MQ=255)
                                                                 aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          >  1:1307244/1‑109 (MQ=255)
                                                                 aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          <  2:1307244/109‑1 (MQ=255)
                                                                 aaGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgc                                                            <  1:83459/139‑1 (MQ=255)
                                                                    cTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  >  1:825569/1‑98 (MQ=255)
                                                                    cTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGcc                                                                                                  <  2:825569/98‑1 (MQ=255)
                                                                    cTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagc                                                         <  1:789448/139‑1 (MQ=255)
                                                                     tGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagca                                                        <  2:1571718/139‑1 (MQ=255)
                                                                     tGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcagca                                                        <  1:719176/139‑1 (MQ=255)
                                                                              ttACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                               <  2:279905/139‑1 (MQ=255)
                                                                               tACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGc                                              >  1:1225176/1‑139 (MQ=255)
                                                                                 ccGTGCAGCAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTcc                                                                                                                     <  2:407049/66‑1 (MQ=255)
                                                                                 ccGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTcc                                                                                                                     >  1:407049/1‑66 (MQ=255)
                                                                                      cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                      >  1:512774/1‑44 (MQ=255)
                                                                                      cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGaa                                                                                                                                      <  2:512774/44‑1 (MQ=255)
                                                                                      cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   >  2:1521193/1‑63 (MQ=255)
                                                                                      cAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTg                                                                                                                   <  1:1521193/63‑1 (MQ=255)
                                                                                           gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACTGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATg                                  <  2:1225176/139‑1 (MQ=255)
                                                                                           gTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATg                                  <  1:859612/139‑1 (MQ=255)
                                                                                              gCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag                                                          <  1:1233914/112‑1 (MQ=255)
                                                                                              gCTCGGCTGTTCGATTCAGAAATGGAAGCCATCGAAGGGGGCTGGAGCGCTCCCGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATgcag                                                          >  2:1233914/1‑112 (MQ=255)
                                                                                                      gTTCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACtt                       >  1:702849/1‑139 (MQ=255)
                                                                                                       ttCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgt                                                                                             <  2:888139/68‑1 (MQ=255)
                                                                                                       ttCGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTc                      >  1:1353011/1‑139 (MQ=255)
                                                                                                         cGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          >  2:1633579/1‑69 (MQ=255)
                                                                                                         cGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTa                                                   <  1:1057211/108‑1 (MQ=255)
                                                                                                         cGATTCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTa                                                   >  2:1057211/1‑108 (MQ=255)
                                                                                                            ttCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                               <  2:818331/109‑1 (MQ=255)
                                                                                                            ttCAGAACTGGAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATggg                                               >  1:818331/1‑109 (MQ=255)
                                                                                                                      gAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCgg                  >  1:544355/1‑128 (MQ=255)
                                                                                                                      gAAGCCATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTc       <  2:14628/139‑1 (MQ=255)
                                                                                                                      gAAGCCATCGAAGGGGGCTGGAGCGATCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCgg                  <  2:544355/128‑1 (MQ=255)
                                                                                                                           cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAAtgtgtg                                                                                          >  1:222980/1‑51 (MQ=255)
                                                                                                                           cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  1:527977/139‑1 (MQ=255)
                                                                                                                           cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  2:702849/139‑1 (MQ=255)
                                                                                                                           cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  1:649423/139‑1 (MQ=255)
                                                                                                                           cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  >  2:649423/1‑139 (MQ=255)
                                                                                                                           cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  <  2:1250899/139‑1 (MQ=255)
                                                                                                                           cATCGAAGGGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCgg  >  1:1363799/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                   
TGTAAAACTGTACGAAATTATCGATATTGTTTCCCTTGAACATGGTGTTACGTTAGCGAAAACAGAAGCTGAACACGTTTACCGTGCAGAAGTCGCTCGGCTGTTCGATTCAGAACTGGAAGCCATCGAA‑GGGGCTGGAGCGCTCCTGTCAGCGATCACTGCGCCAATGTGTGTGGTATCTAACGGCCCAAATAACAAAATGCAGCATTCTATGGGCAAGCTGAATATGTTGCACTACTTCCCGGATAAACTGTTCAGCGG  >  NC_000913/3896917‑3897177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑