New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1390544 =41 (0.600)4 (0.060) 4/202 6.7 8.5% coding (55/729 nt) mpaA murein peptide amidase A
?NC_000913 1390553 = 45 (0.670)coding (46/729 nt) mpaA murein peptide amidase A
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGCCTGTAATACTTCCTGAACCAGAGACGATCATGACCGTAACCCGCCCACGCGCCGAACGCGGCGCATTTCCGCCCGGAACAGAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1390630‑1390544
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cCGGGCGGAAATGCGCCGCGTTCGGCGCG  >  NC_000913/1390553‑1390580
                                                                                                                   
TGCCTGTAATACTTCCTGAACCAGAGACGATCATGACCGTAACCCGCCCACGCGCCGAACGCGGCGCATTTCCGCCCGGAACAGAACCGGGCGGAAATGCGCCGCGTTCGGCGCG  >  1:952352/1‑115
TGCCTGTAATACTTCCTGAACCAGAGACGATCATGACCGTAACCCGCCCACGCGCCGAACGCGGCGCATTTCCGCCCGGAACAGAACCGGGCGGAAATGCGCCGCGTTCGGCGCG  <  2:952352/115‑1
         TACTTCCTGAACCAGAGACGATCATGACCGTAACCCGCCCACGCGCCGAACGCGGCGCATTTCCGCCCGGAACAGAAC‑‑GGCGGAAATGCGCCGCGTT         <  1:498485/97‑1
         TACTTCCTGAACCAGAGACGATCATGACCGTAACCCGCCCACGCGCCGAACGCGGCGCATTTCCGCCCGGAACAGAAC‑‑GGCGGAAATGCGCCGCGTT         >  2:498485/1‑97
                                                                                                                   
TGCCTGTAATACTTCCTGAACCAGAGACGATCATGACCGTAACCCGCCCACGCGCCGAACGCGGCGCATTTCCGCCCGGAACAGAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1390630‑1390544
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cCGGGCGGAAATGCGCCGCGTTCGGCGCG  >  NC_000913/1390553‑1390580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.