| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | NC_000913 | 2,893,961 | Δ3 bp | coding (43‑45/261 nt) | ygcO → | putative 4Fe‑4S cluster‑containing protein |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 2893961 | 2893963 | 3 | 85 [0] | [0] 85 | ygcO | putative 4Fe‑4S cluster‑containing protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 2893960 | 0 (0.000) | 85 (1.250) | 52/204 | 0.1 | 100% | coding (42/261 nt) | ygcO | putative 4Fe‑4S cluster‑containing protein |
| ? | NC_000913 | 2893964 = | 0 (0.000) | coding (46/261 nt) | ygcO | putative 4Fe‑4S cluster‑containing protein | |||||
ATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCT > NC_000913/2893825‑2894096 | aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGatt > 1:631345‑M1/1‑136 (MQ=255) ttCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggct > 2:917496‑M1/1‑127 (MQ=255) ccACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt < 1:1051433‑M1/131‑7 (MQ=255) ccACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCCGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt > 2:1051433‑M1/1‑125 (MQ=255) ccGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactc > 1:350633‑M1/1‑122 (MQ=255) gCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcag < 2:350633‑M1/139‑38 (MQ=255) catcatTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcaga > 2:670377‑M1/1‑101 (MQ=255) catcatTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt < 2:1673269‑M1/107‑7 (MQ=255) catcatTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt > 1:1673269‑M1/1‑101 (MQ=255) tcatTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaac < 1:670377‑M1/139‑41 (MQ=255) atTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacgg > 2:588426‑M1/1‑97 (MQ=255) cgtcgtCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGat < 1:250216‑M1/94‑3 (MQ=255) cgtcgtCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGat > 2:250216‑M1/1‑92 (MQ=255) gtcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTCGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaac < 1:917496‑M1/139‑52 (MQ=255) tcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacg > 1:1588427‑M1/1‑87 (MQ=255) tcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaac < 2:460501‑M1/138‑52 (MQ=255) tcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaac > 1:460501‑M1/1‑87 (MQ=255) tcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgact > 1:1648738‑M1/1‑87 (MQ=255) tcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgact < 2:1648738‑M1/103‑17 (MQ=255) tcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGa < 1:301928‑M1/88‑2 (MQ=255) tcACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGa > 2:301928‑M1/1‑87 (MQ=255) aCGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt > 2:1010998‑M1/1‑85 (MQ=255) aCGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt < 1:1010998‑M1/91‑7 (MQ=255) tgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggc > 2:531926‑M1/1‑77 (MQ=255) tgGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggc > 2:325174‑M1/1‑77 (MQ=255) tCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtc > 1:1658254‑M1/1‑72 (MQ=255) cTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtct > 1:1705861‑M1/1‑71 (MQ=255) cTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtct > 2:1394492‑M1/1‑71 (MQ=255) cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGa > 1:1527490‑M1/1‑67 (MQ=255) cgggcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGa < 2:1527490‑M1/68‑2 (MQ=255) gcgATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcg < 1:1433615‑M1/139‑76 (MQ=255) gATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgct < 2:1588427‑M1/139‑78 (MQ=255) gATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgct < 2:245273‑M1/139‑78 (MQ=255) gATGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgct < 1:531926‑M1/139‑78 (MQ=255) tGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctca < 1:588426‑M1/139‑80 (MQ=255) tGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt < 2:736815‑M1/66‑7 (MQ=255) tGTTATCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgtt > 1:736815‑M1/1‑60 (MQ=255) aTCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacacc < 1:748995‑M1/139‑84 (MQ=255) tCAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccg < 2:1705861‑M1/139‑85 (MQ=255) cAGGAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgg < 2:1658254‑M1/139‑86 (MQ=255) gAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaag > 1:827242‑M1/1‑51 (MQ=255) gAGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaag > 1:1601129‑M1/1‑51 (MQ=255) aGTCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaagg > 2:256584‑M1/1‑50 (MQ=255) tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggt > 1:1295544‑M1/1‑48 (MQ=255) tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggt < 2:1295544‑M1/114‑67 (MQ=255) tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa < 2:32046‑M1/87‑40 (MQ=255) tCTGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa > 1:32046‑M1/1‑48 (MQ=255) tGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaac > 1:1641009‑M1/1‑46 (MQ=255) tGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgct < 2:334564‑M1/123‑78 (MQ=255) tGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgct > 1:334564‑M1/1‑46 (MQ=255) tGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgact > 1:147344‑M1/1‑46 (MQ=255) tGCGATGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgact < 2:147344‑M1/62‑17 (MQ=255) tGTCTGTAACCCGTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaacttacg > 1:1370398‑M1/1‑41 (MQ=255) taacccgtaATCTCTGGCGCGTTGCTGATGCGCCGattgtt > 1:296528‑M1/4‑35 (MQ=255) taacccgtaATCTCTGGCGCGTTGCTGATGCGCCGattgtt < 2:296528‑M1/38‑7 (MQ=255) gTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtcctgcaggtcttttttcgctcacaccggaag > 1:1094973‑M1/1‑29 (MQ=255) gTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccgtcaggtcttttttcgctc < 2:992270‑M1/107‑79 (MQ=255) gTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctc > 1:992270‑M1/1‑29 (MQ=255) gTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa < 2:680467‑M1/68‑40 (MQ=255) gTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaa > 1:680467‑M1/1‑29 (MQ=255) gTAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggatgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaag < 2:1094973‑M1/117‑89 (MQ=255) tAATCTCTGGCGCGTTGCTGATGCGCCGattgttccggctgactccgtcgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaacttacgtattgactatcgc > 2:919166‑M1/1‑28 (MQ=255) tGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaacttacgtattgactatcgcagttgcc > 1:1241672‑M1/1‑21 (MQ=255) tGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttt < 1:1042498‑M1/93‑73 (MQ=255) tGGCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtctattt > 2:1042498‑M1/1‑21 (MQ=255) ggCGCGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaacttacgtattgactatcgcagttgcct < 1:1394492‑M1/139‑120 (MQ=255) gcgcGTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaacttacgtattgactatcgcagttgcctg < 1:325174‑M1/139‑121 (MQ=255) cgcgTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcaca < 2:781802‑M1/99‑82 (MQ=255) cgcgTTGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcaca > 1:781802‑M1/1‑18 (MQ=255) ttGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaacttacgtattgactatcgcagttgcctggagtg > 2:705032‑M1/1‑14 (MQ=255) ttGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggt > 2:1555350‑M1/1‑14 (MQ=255) ttGCTGATGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggt < 1:1555350‑M1/57‑44 (MQ=255) aTGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggt < 1:748666‑M1/98‑91 (MQ=255) aTGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggt > 2:748666‑M1/1‑8 (MQ=255) tGCGCCGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctatccggcaggtcttttttcgctcacaccggaaggtaacttacgtattgactatcgcagttgcctggagtgtggcacc < 2:1241672‑M1/139‑133 (MQ=255) ccGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaacttacgtattgactatcgcagttgcctggagtgtggcacctgcc > 1:170656‑M1/1‑3 (MQ=255) ccGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaaggtaacttacgtatagactatcgcagttgcctggagtgtggcacctgcc > 1:1111916‑M1/1‑3 (MQ=255) ccGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaa < 2:526306‑M1/90‑88 (MQ=255) ccGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccggaa > 1:526306‑M1/1‑3 (MQ=255) ccGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgga < 1:181382‑M1/89‑87 (MQ=255) ccGattgttccggctgactccgttgagcgccagacggcagaacggttgattaacgcctgtccggcaggtcttttttcgctcacaccgga > 2:181382‑M1/1‑3 (MQ=255) ccGattgttccggctgactccgttgagcgccagacggcagaa > 1:981313‑M1/1‑3 (MQ=255) ccGattgttccggctgactccgttgagcgccagacggcagaa < 2:981313‑M1/42‑40 (MQ=255) ccGattgttccggctgactccgttgagcgccagacggcag < 1:129648‑M1/40‑38 (MQ=255) ccGattgttccggctgactccgttgagcgccagacggcag > 2:129648‑M1/1‑3 (MQ=255) | ATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGGCGATGTTATCAGGAGTCTGCGATGTCTGTAGCCCGTAATCTCTGGCGCGTTGCTGATGCGCCGCACATTGTTCCGGCTGACTCCGTTGAGCGCCAGACGGCAGAACGGTTGATTAACGCCTGTCCGGCAGGTCTTTTTTCGCTCACACCGGAAGGTAACTTACGTATTGACTATCGCAGTTGCCTGGAGTGTGGCACCT > NC_000913/2893825‑2894096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |