Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 49,767 A→G intergenic (+136/‑56) kefC → / → folA potassium:proton antiporter/dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091349,7670AG100.0% 246.8 / NA 67intergenic (+136/‑56)kefC/folApotassium:proton antiporter/dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/33);  total (34/33)

AGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTG  >  NC_000913/49637‑49894
                                                                                                                                  |                                                                                                                               
agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                         >  1:785458/1‑139 (MQ=255)
agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                         >  2:1615998/1‑139 (MQ=255)
agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                         >  2:1704902/1‑139 (MQ=255)
 gTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGttt                                                                                                                        <  1:1704902/139‑1 (MQ=255)
  tGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGGACCAGTTGGCGACGGTTTa                                                                                                                       <  1:1154783/139‑1 (MQ=255)
  tGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTa                                                                                                                       >  2:1154783/1‑139 (MQ=255)
   gACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTAc                                                                                                                      <  2:785458/139‑1 (MQ=255)
     cGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    <  1:1615998/139‑1 (MQ=255)
       tAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCtt                                                                                                                  <  2:199743/139‑1 (MQ=255)
       tAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCtt                                                                                                                  <  2:834415/139‑1 (MQ=255)
        aaaTCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCttt                                                                                                                 <  2:1079089/139‑1 (MQ=255)
             acacTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGta                                                                                                            <  2:1721182/139‑1 (MQ=255)
              cacTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGtat                                                                                                           >  2:723709/1‑139 (MQ=255)
                cTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAg                                                                                                         >  2:94151/1‑139 (MQ=255)
                        cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACa                                                                                                 >  1:537835/1‑139 (MQ=255)
                          aaCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    >  1:86016/1‑118 (MQ=255)
                          aaCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    <  2:86016/118‑1 (MQ=255)
                           aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCttt                                                                                                                 >  2:52676/1‑120 (MQ=255)
                           aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCttt                                                                                                                 <  1:52676/120‑1 (MQ=255)
                           aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCtt                                                                                                                  >  2:967489/1‑119 (MQ=255)
                           aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCtt                                                                                                                  <  1:967489/119‑1 (MQ=255)
                           aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAtt                                                                                              >  2:820271/1‑139 (MQ=255)
                           aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAtt                                                                                              >  1:113079/1‑139 (MQ=255)
                           aCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATCTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAtt                                                                                              >  2:474579/1‑139 (MQ=255)
                             tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAAtttt                                                                                            >  1:1167707/1‑139 (MQ=255)
                                                      cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCa                                                                   >  2:464509/1‑139 (MQ=255)
                                                        gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACg                                                                                                                     <  1:1321024/87‑1 (MQ=255)
                                                        gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACg                                                                                                                     >  2:1321024/1‑87 (MQ=255)
                                                        gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                                                                 >  2:1584021/1‑139 (MQ=255)
                                                        gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGt                                                                 >  1:1448313/1‑139 (MQ=255)
                                                         cgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                         >  1:75954/1‑82 (MQ=255)
                                                         cgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGtt                                                                                                                         <  2:75954/82‑1 (MQ=255)
                                                         cgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTc                                                                >  2:1195226/1‑139 (MQ=255)
                                                                 gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg                                                        >  2:261829/1‑139 (MQ=255)
                                                                 gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATCTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg                                                        <  1:474579/139‑1 (MQ=255)
                                                                   gagagaGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggc                                                      <  2:537835/139‑1 (MQ=255)
                                                                   gagagaGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggc                                                      <  1:94151/139‑1 (MQ=255)
                                                                   gagagaGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggc                                                      <  2:1448313/139‑1 (MQ=255)
                                                                     gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                    <  1:1195226/139‑1 (MQ=255)
                                                                     gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCAACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                    <  2:1167707/139‑1 (MQ=255)
                                                                      agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt                                                   >  2:1580959/1‑139 (MQ=255)
                                                                       gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   >  1:1551710/1‑74 (MQ=255)
                                                                       gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCt                                                                                                                   <  2:1551710/74‑1 (MQ=255)
                                                                          cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                    >  2:989110/1‑134 (MQ=255)
                                                                          cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                    <  1:989110/134‑1 (MQ=255)
                                                                           gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    >  1:435140/1‑69 (MQ=255)
                                                                           gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGc                                                                                                                    <  2:435140/69‑1 (MQ=255)
                                                                              gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTa                                                                                                                       <  2:12716/63‑1 (MQ=255)
                                                                              gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTa                                                                                                                       >  1:12716/1‑63 (MQ=255)
                                                                                   gCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTa                                                                                                                       >  2:1213599/1‑58 (MQ=255)
                                                                                   gCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTa                                                                                                                       <  1:1213599/58‑1 (MQ=255)
                                                                                   gCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATcgc                                      >  1:1077186/1‑139 (MQ=255)
                                                                                       gcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa                                  <  1:1580959/139‑1 (MQ=255)
                                                                                        cagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTAt                                 <  1:820271/139‑1 (MQ=255)
                                                                                                     tttCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGaaa                      >  2:1687429/1‑137 (MQ=255)
                                                                                                     tttCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGaaa                      <  1:1687429/137‑1 (MQ=255)
                                                                                                     tttCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAc                    >  2:1138231/1‑139 (MQ=255)
                                                                                                       tCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                 >  1:1005942/1‑76 (MQ=255)
                                                                                                       tCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg                                                        >  2:1409350/1‑101 (MQ=255)
                                                                                                       tCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg                                                        <  1:1409350/101‑1 (MQ=255)
                                                                                                       tCCTCAACATCATCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTGTTTTAtagcga                                                                                 <  2:1005942/76‑6 (MQ=255)
                                                                                                              catcatCCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCg           <  2:113079/139‑1 (MQ=255)
                                                                                                                 catcCTCGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTgg        >  2:864155/1‑139 (MQ=255)
                                                                                                                       cGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctg  <  1:723709/139‑1 (MQ=255)
                                                                                                                       cGCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctg  <  1:464509/139‑1 (MQ=255)
                                                                                                                        gCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc                                                >  1:917161/1‑92 (MQ=255)
                                                                                                                        gCACCAGTTGGCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc                                                <  2:917161/92‑1 (MQ=255)
                                                                                                                                  |                                                                                                                               
AGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTG  >  NC_000913/49637‑49894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: