Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	544,754	C→T	R180H (CGT→CAT)	allC ←	allantoate amidohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	544,754	0	C	T	100.0%	215.6 / NA	66	R180H (CGT→CAT)	allC	allantoate amidohydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/32);  total (34/32)

TTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCA  >  NC_000913/544620‑544887                                                                                                                                       |                                                                                                                                      ttGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCgg                                                                                                                                   <  1:410655/139‑1 (MQ=255)  tGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGa                                                                                                                                  <  2:425511/139‑1 (MQ=255)      tCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTa                                                                                                                              <  2:11839/139‑1 (MQ=255)                      ccGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTCCTTTCCAGCACACACCCCTGTTCAATATGCAGATCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAag                                                                                                              >  2:789857/1‑139 (MQ=255)                      ccGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAag                                                                                                              >  1:1658920/1‑139 (MQ=255)                           taACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTaa                                                                                                         >  2:718131/1‑139 (MQ=255)                                acgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTAAACAAAGGCTTTAATATCCTGATGCgg                                                                                                                                   <  1:57029/108‑1 (MQ=255)                                acgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTAAACAAAGGCTTTAATATCCTGATGCgg                                                                                                                                   >  2:57029/1‑108 (MQ=255)                                 cgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGc                                                                                                   <  2:1658920/139‑1 (MQ=255)                                  gCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCa                                                                                                  <  1:718131/139‑1 (MQ=255)                                   cTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGc                                                                                                                       <  1:1385924/117‑1 (MQ=255)                                   cTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGc                                                                                                                       >  2:1385924/1‑117 (MQ=255)                                   cTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCaa                                                                                                 >  2:1354802/1‑139 (MQ=255)                                                   ttCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgc                                                                                      >  2:811973/1‑134 (MQ=255)                                                   ttCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTcatcgc                                                                                      <  1:811973/134‑1 (MQ=255)                                                      accacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaa                                                                              <  1:745257/139‑1 (MQ=255)                                                         acGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCg                                                                                                                      >  1:1687434/1‑96 (MQ=255)                                                         acGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCg                                                                                                                      <  2:1687434/96‑1 (MQ=255)                                                         acGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGc                                                                                                   <  1:709619/115‑1 (MQ=255)                                                         acGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGc                                                                                                   >  2:709619/1‑115 (MQ=255)                                                          cGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCaa                                                                                                 >  2:516243/1‑116 (MQ=255)                                                          cGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCaa                                                                                                 <  1:516243/116‑1 (MQ=255)                                                           gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGtt                                                                                                                    >  2:456602/1‑96 (MQ=255)                                                           gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGtt                                                                                                                    <  1:456602/96‑1 (MQ=255)                                                           gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTa                                                                         >  2:265349/1‑139 (MQ=255)                                                                     tgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTgg                                                               <  2:223406/139‑1 (MQ=255)                                                                      gCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGc                                                              >  1:1385512/1‑139 (MQ=255)                                                                      gCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGc                                                              <  2:1385512/139‑1 (MQ=255)                                                                       cccATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCg                                                                                                    <  1:194116/100‑1 (MQ=255)                                                                       cccATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCg                                                                                                    >  2:194116/1‑100 (MQ=255)                                                                         cATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGtt                                                                                                                    >  1:1053250/1‑82 (MQ=255)                                                                         cATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGtt                                                                                                                    <  2:1053250/82‑1 (MQ=255)                                                                             aCTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACa                                                                               <  1:1727217/115‑1 (MQ=255)                                                                             aCTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACa                                                                               >  2:1727217/1‑115 (MQ=255)                                                                                 tCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCgg                                                                                                                 <  1:1672524/77‑1 (MQ=255)                                                                                 tCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCgg                                                                                                                 >  2:1672524/1‑77 (MQ=255)                                                                                        cacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGT‑GGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTCGCATCACAGATATTCCGCAcg                                           >  2:1016391/1‑139 (MQ=255)                                                                                           aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGc                                                                                               <  2:945654/85‑1 (MQ=255)                                                                                           aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGc                                                                                               >  1:945654/1‑85 (MQ=255)                                                                                                       tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCgg                                                                                                                                   <  1:322650/37‑1 (MQ=38)                                                                                                       tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCgg                                                                                                                                   >  2:322650/1‑37 (MQ=38)                                                                                                       tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaa                                                                              <  1:1629735/90‑1 (MQ=255)                                                                                                       tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaa                                                                              >  2:1629735/1‑90 (MQ=255)                                                                                                          gCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAgg                                   <  2:23539/130‑1 (MQ=255)                                                                                                          gCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAgg                                   >  1:23539/1‑130 (MQ=255)                                                                                                            aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                            <  2:1706480/103‑1 (MQ=255)                                                                                                            aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                            >  1:1706480/1‑103 (MQ=255)                                                                                                            aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc                        >  1:1155596/1‑139 (MQ=255)                                                                                                            aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc                        >  1:1332985/1‑139 (MQ=255)                                                                                                               tCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGa                                                                                 <  2:1616165/79‑1 (MQ=255)                                                                                                               tCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCCTCATCGCATCGa                                                                                 >  1:1616165/1‑79 (MQ=255)                                                                                                                 aacaaAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaaaa                                                                            <  1:796369/82‑1 (MQ=255)                                                                                                                 aacaaAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaaaa                                                                            >  2:796369/1‑82 (MQ=255)                                                                                                                 aacaaAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCa                                             <  2:1584/113‑1 (MQ=255)                                                                                                                 aacaaAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCa                                             >  1:1584/1‑113 (MQ=255)                                                                                                                        gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTgg                                                               <  1:301588/88‑1 (MQ=255)                                                                                                                        gCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTgg                                                               >  2:301588/1‑88 (MQ=255)                                                                                                                        gCTTTAATATCCTGATGCGGAGTTAGGGGGGGGGTCGGAAGAGTAAATCCGAAAGCATAAAGCGAAAGGAGAAAACTAAGGGCTTTGGCATCACAGAGATTAAGGACGTCGTGAGGATTCGACAGCCAAAAAATAttat            >  2:173920/1‑137 (MQ=255)                                                                                                                         cTTTAATATCCTGATGCGGAGTTAGT‑GGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTa          <  1:1016391/139‑1 (MQ=255)                                                                                                                           ttAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGc                                                              >  1:1371099/1‑86 (MQ=255)                                                                                                                           ttAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGc                                                              <  2:1371099/86‑1 (MQ=255)                                                                                                                                  ccTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCaa                                                                                                 <  1:712247/44‑1 (MQ=255)                                                                                                                                  ccTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCaa                                                                                                 >  2:712247/1‑44 (MQ=255)                                                                                                                                  ccTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGc                                                              >  2:1491040/1‑79 (MQ=255)                                                                                                                                  ccTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGc                                                              <  1:1491040/79‑1 (MQ=255)                                                                                                                                  ccTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCa  >  1:959812/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                      TTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCA  >  NC_000913/544620‑544887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑