Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	875,327	G→A	*783* (TAG→TAA)	yliE →	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	875,327	0	G	A	100.0%	194.1 / NA	61	*783* (TAG→TAA)	yliE	putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/30);  total (31/30)

TTGCGGAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAGTTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCC  >  NC_000913/875191‑875457                                                                                                                                         |                                                                                                                                   ttGCGGAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAAtt                                                                                                                                  <  1:983345/139‑1 (MQ=255)     ggAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACg                                                                                                                              <  2:372224/139‑1 (MQ=255)      gAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGCTGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt                                                                                                                             <  1:1033050/139‑1 (MQ=255)                    cGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt                                                                                                                             <  2:1467644/125‑1 (MQ=255)                    cGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt                                                                                                                             >  1:1467644/1‑125 (MQ=255)                          cGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGtt                                                                                                         >  1:730293/1‑139 (MQ=255)                             tGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGt                                                                                                      >  1:7360/1‑139 (MQ=255)                              gcgcgcGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTa                                                                                                     >  1:50620/1‑139 (MQ=255)                                               aTTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTATTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTctgct                                                                                    <  2:792133/139‑1 (MQ=255)                                                    gATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGAttt                                                                               >  1:331040/1‑139 (MQ=255)                                                    gATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGGAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGAttt                                                                               >  1:1083466/1‑139 (MQ=255)                                                         gtTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATc                                                                          <  1:285688/139‑1 (MQ=255)                                                           tCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCaa                                                                        <  2:1083466/139‑2 (MQ=255)                                                           tCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCAt                                                                        <  2:331040/139‑1 (MQ=255)                                                             aGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACg                                                                                                                              >  1:209258/1‑83 (MQ=255)                                                             aGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACg                                                                                                                              <  2:209258/83‑1 (MQ=255)                                                                  cAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTCTGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTca                                                                 >  2:1294068/1‑139 (MQ=255)                                                                  cAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTca                                                                 >  2:739075/1‑139 (MQ=255)                                                                    ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAg                                                                                                                     >  2:725693/1‑85 (MQ=255)                                                                    ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAg                                                                                                                     <  1:725693/85‑1 (MQ=255)                                                                    ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTa                                                                                                     <  2:773924/101‑1 (MQ=255)                                                                    ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTa                                                                                                     >  1:773924/1‑101 (MQ=255)                                                                    ggggTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTcagc                                                               >  2:1456196/1‑139 (MQ=255)                                                                          tCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGc                                                         <  1:739075/139‑1 (MQ=255)                                                                             gTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt                                                                                                                             >  1:665123/1‑68 (MQ=255)                                                                             gTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGt                                                                                                                             <  2:665123/68‑1 (MQ=255)                                                                             gTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTg                                                      <  2:7360/139‑1 (MQ=255)                                                                               aCCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCg                                                    <  2:730293/139‑1 (MQ=255)                                                                                    aaaCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAg                                                                                                           >  1:1296944/1‑79 (MQ=255)                                                                                    aaaCCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAg                                                                                                           <  2:1296944/79‑1 (MQ=255)                                                                                           tGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGc                                                     <  2:1217228/126‑1 (MQ=255)                                                                                           tGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGc                                                     >  1:1217228/1‑126 (MQ=255)                                                                                            gCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCg                                                    >  2:341661/1‑126 (MQ=255)                                                                                            gCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCg                                                    <  1:341661/126‑1 (MQ=255)                                                                                                 tctcCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCAt                                                                        >  1:1463701/1‑101 (MQ=255)                                                                                                 tctcCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCAt                                                                        <  2:1463701/101‑1 (MQ=255)                                                                                                  ctcCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGt                                 >  1:1314515/1‑139 (MQ=255)                                                                                                  ctcCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGt                                 <  2:50620/139‑1 (MQ=255)                                                                                                     cGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTcag                                                                >  1:1527086/1‑105 (MQ=255)                                                                                                     cGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTcag                                                                <  2:1527086/105‑1 (MQ=255)                                                                                                     cGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCaaa                                     >  1:179979/1‑132 (MQ=255)                                                                                                     cGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCaaa                                     <  2:179979/132‑1 (MQ=255)                                                                                                     cGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTaaa                              >  2:203028/1‑139 (MQ=255)                                                                                                       ccTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg                            >  1:930046/1‑139 (MQ=255)                                                                                                         tGGGATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGaa                          <  1:1294068/139‑1 (MQ=255)                                                                                                          gggATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTAcagt                                                                                             >  2:844107/1‑71 (MQ=255)                                                                                                          gggATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTAcagt                                                                                             <  1:844107/71‑1 (MQ=255)                                                                                                          gggATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg                            <  1:1613645/136‑1 (MQ=255)                                                                                                          gggATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAAc                         <  1:203028/139‑1 (MQ=255)                                                                                                          gggATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTACAGAATCAATAAGTTCGTACTTACAGGCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAgg                            >  2:1613645/1‑136 (MQ=255)                                                                                                           ggATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACg                        >  2:1063569/1‑139 (MQ=255)                                                                                                            gATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCt                                                       >  2:395736/1‑107 (MQ=255)                                                                                                            gATAAAAGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCt                                                       <  1:395736/107‑1 (MQ=255)                                                                                                                 aaGTGGAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGta                  >  2:1377072/1‑139 (MQ=255)                                                                                                                     ggAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGc              >  1:372933/1‑139 (MQ=255)                                                                                                                     ggAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGc              >  1:108899/1‑139 (MQ=255)                                                                                                                      gAAAATTAGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGcc             >  2:195424/1‑139 (MQ=255)                                                                                                                             aGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACa      >  1:872056/1‑139 (MQ=255)                                                                                                                             aGTAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACa      <  1:195424/139‑1 (MQ=255)                                                                                                                               tAAAAGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGt    <  1:246056/139‑1 (MQ=255)                                                                                                                                 aaaGAGTAATTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTcc  <  1:1377072/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   TTGCGGAAGGCGTAGAGAGCGAAGACGTTGCGCGCGAATTAATCAAATTAGGATGTGTTCAGGCTCAGGGGTATCTGTACCAGAAACCCATGCCATTCTCCGCCTGGGATAAAAGTGGAAAATTAGTAAAAGAGTAGTTTACGTATGTCCAGAATCAATAAGTTCGTACTTACAGTCAGTCTGCTGATTTTTATCATGATTTCAGCAGTTGCCTGCGGGATCTACACTCAAATGGTAAAGGAACGGGTGTATAGCCTGAAACAGTCC  >  NC_000913/875191‑875457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑