| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 889,215 | A→G | L202P (CTG→CCG) | ybjL ← | putative transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 889,215 | 0 | A | G | 94.3% | 167.3 / ‑0.4 | 53 | L202P (CTG→CCG) | ybjL | putative transporter |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (24/26); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01 | |||||||||||
ATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCAGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGATTAAATAGGTTAAGGCATACCCGAGGCTCAGATT > NC_000913/889083‑889348 | aTGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCAc > 1:1678997/1‑139 (MQ=255) gcagcCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTcg > 1:594674/1‑139 (MQ=255) ccGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCggg > 2:986294/1‑139 (MQ=255) ggTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCg > 2:133643/1‑139 (MQ=255) aaaTACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGgc < 1:133643/139‑1 (MQ=255) aaTACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGgcg < 2:198146/139‑1 (MQ=255) aaTACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGgcg < 1:1012939/139‑1 (MQ=255) cccAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTgg > 1:1557123/1‑139 (MQ=255) gTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTg < 1:1355919/139‑1 (MQ=255) ttCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCTGGGCCACGTTcg < 1:439214/112‑1 (MQ=255) ttCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTcg > 2:439214/1‑112 (MQ=255) cGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGt > 1:1459468/1‑139 (MQ=255) cGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGt > 1:261632/1‑139 (MQ=255) caccaGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTaa < 2:594674/139‑1 (MQ=255) caGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTcgc > 1:943861/1‑80 (MQ=255) caGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTcgc < 2:943861/80‑1 (MQ=255) caGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCAGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAAcgc > 2:250743/1‑139 (MQ=255) aGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAAcgcg < 2:1555918/139‑1 (MQ=255) aGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAAcgcg < 1:986294/139‑1 (MQ=255) aGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAAcgcg < 1:435925/139‑1 (MQ=255) aGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCAGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAAcgcg < 2:775467/139‑1 (MQ=255) ccGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCAcc < 2:1678997/139‑1 (MQ=255) ggCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACcaac > 2:1353717/1‑139 (MQ=255) ccGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACcaacaa < 2:1557123/139‑1 (MQ=255) ccGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACcaacaa < 1:477090/139‑1 (MQ=255) aCGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGt > 2:1549927/1‑93 (MQ=255) aCGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGt < 1:1549927/93‑1 (MQ=255) aTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAAtt < 2:1625365/107‑1 (MQ=255) aTAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAAtt > 1:1625365/1‑107 (MQ=255) cgctGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCaaa < 1:1121895/131‑1 (MQ=255) cgcgGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCaaa > 2:1121895/1‑131 (MQ=255) gATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGgcg > 1:320352/1‑70 (MQ=255) gATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGgcg < 2:320352/70‑1 (MQ=255) gATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACgcgc > 1:1198846/1‑114 (MQ=255) gATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACgcgc < 2:1198846/114‑1 (MQ=255) cGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAAtt < 1:1183382/92‑1 (MQ=255) cGGTAAATAAACCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAAtt > 2:1183382/1‑92 (MQ=255) cGGTAAATAAACCTTACGGTTGGCATCAGTGTCCAGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGATTaaa < 1:250743/139‑1 (MQ=255) aaCCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAg < 1:404786/120‑1 (MQ=255) aaCCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAg > 2:404786/1‑120 (MQ=255) aCCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGa > 2:1252211/1‑124 (MQ=255) aCCTTACGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGa < 1:1252211/124‑1 (MQ=255) cGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAAtt > 2:1165605/1‑76 (MQ=255) cGGTTGGCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAAtt < 1:1165605/76‑1 (MQ=255) ggCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGATTAAATAGGTTAAGGCATAccc < 2:1149676/135‑1 (MQ=255) ggCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGATTAAATAGGTTAAGGCATAccc > 1:1149676/1‑135 (MQ=255) ggCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGATTAAATAGGTTAAGGCATACCCGAgg > 2:1300457/1‑139 (MQ=255) gCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACcaacaa < 1:1710483/94‑1 (MQ=255) gCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACcaacaa > 2:1710483/1‑94 (MQ=255) gCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGATTAAATAGGTTAAGGCATACCCGAGGc > 1:979596/1‑139 (MQ=255) gCATCAGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGATTAAATAGGTTAAGGCATACCCGAGGc < 1:1103356/139‑1 (MQ=255) aGTGTCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGATTAAATAGGTTAAGGCATACCCGAGGCTCAGa < 1:1300457/139‑1 (MQ=255) tgtCCGGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGATTAAATAGGTTAAGGCATACCCGAGGCTCAGAtt > 1:1599371/1‑139 (MQ=255) | ATGTAGCAGCCGGTTTGTCGATAAATACCCAGTTCACGCAGATTTTTGCCGTCGGTCCAGGCCACCAGTTCCGGGCCGACGCGATAGGCGCGGATCACCGGTAAATAAACCTTACGGTTGGCATCAGTGTCCAGGCCACGTTCGCGGGCGATTTGCTGGGCGCTGGTCTGTAAGTCCTGATGCTGCAATTTCGGCAAGTAACGCGCACCAACAATCAAACTCACCAGACCGATTAAATAGGTTAAGGCATACCCGAGGCTCAGATT > NC_000913/889083‑889348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |