Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	905,743	G→A	*277* (TAG→TAA)  R337R (CGC→CGT)	amiD → ybjS ←	1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent, OM lipoprotein putative NAD(P)H‑dependent oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	905,743	0	G	A	98.7%	237.1 / NA	74	*277* (TAG→TAA)  R337R (CGC→CGT)	amiD ybjS	1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent, OM lipoprotein putative NAD(P)H‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (37/36);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGA  >  NC_000913/905611‑905877                                                                                                                                     |                                                                                                                                       gACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGc                                                                                                                                  >  2:974557/1‑139 (MQ=255)       gcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATACCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGAATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGtttt                                                                                                                            >  2:687949/1‑139 (MQ=255)       gcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAacg                                                                                                                                      >  2:797742/1‑129 (MQ=255)       gcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCgg                                                                                                                                   <  2:7539/132‑1 (MQ=255)       gcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCgg                                                                                                                                   >  1:7539/1‑132 (MQ=255)       gcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAg                                                                                                                                <  1:865696/135‑1 (MQ=255)       gcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAg                                                                                                                                >  2:865696/1‑135 (MQ=255)        cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTc                                                                                                                           >  1:51382/1‑139 (MQ=255)          cgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCg                                                                                                                         <  1:555269/139‑1 (MQ=255)                gcggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTcg                                                                                                                   <  1:1147244/139‑1 (MQ=255)                gcggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTcg                                                                                                                   <  1:687949/139‑1 (MQ=255)                        tGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCa                                                                                                           <  2:51382/139‑1 (MQ=255)                                     ccAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc                                                                                              >  1:326829/1‑139 (MQ=255)                                             aTTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                      <  2:326829/139‑1 (MQ=255)                                               ttCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCtt                                                                                    >  2:568636/1‑139 (MQ=255)                                                                 aaCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCg                                                                  <  2:1337372/139‑1 (MQ=255)                                                                   cGGCGAAGTGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGc                                                                >  2:1543474/1‑139 (MQ=255)                                                                   cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGc                                                                >  1:1005023/1‑139 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCgg                                                                                                                                   >  1:1212041/1‑69 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCgg                                                                                                                                   <  2:1212041/69‑1 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg                                                                                                         <  2:1370832/95‑1 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg                                                                                                         >  1:1370832/1‑95 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                      >  1:916681/1‑114 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                      <  2:916681/114‑1 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCt                                                                                     <  1:321901/115‑1 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCt                                                                                     >  2:321901/1‑115 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             >  1:1585290/1‑139 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             <  2:375913/139‑1 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             >  2:236809/1‑139 (MQ=255)                                                                      cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             >  2:402657/1‑139 (MQ=255)                                                                        aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAg                                                                                                                                >  2:1601064/1‑70 (MQ=255)                                                                        aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAg                                                                                                                                <  1:1601064/70‑1 (MQ=255)                                                                        aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                          >  2:1377963/1‑108 (MQ=255)                                                                        aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                          <  1:1377963/108‑1 (MQ=255)                                                                        aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATa                                                           >  1:139251/1‑139 (MQ=255)                                                                        aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATa                                                           >  1:1412025/1‑139 (MQ=255)                                                                          gCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGTCAGGCGGCAGTTTTCTCGATACATTCATCCAGGGTGATGAACGGCTGATAAc                                                         <  2:1005023/139‑1 (MQ=255)                                                                            ggATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCt                                                       <  2:54729/139‑1 (MQ=255)                                                                                    aaaCTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTc                                               <  1:1543474/139‑1 (MQ=255)                                                                                        tCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTgc                                           >  1:1428622/1‑139 (MQ=255)                                                                                          aGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTgcgc                                         <  2:1428622/139‑1 (MQ=255)                                                                                           ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCa                                        >  1:972643/1‑139 (MQ=255)                                                                                            gCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTaa                                                     <  2:1099236/125‑1 (MQ=255)                                                                                            gCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTaa                                                     >  1:1099236/1‑125 (MQ=255)                                                                                            gCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  1:402657/139‑1 (MQ=255)                                                                                            gCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                       <  1:974557/139‑1 (MQ=255)                                                                                                ttGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTaa                                                     <  2:857521/121‑1 (MQ=255)                                                                                                ttGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTaa                                                     >  1:857521/1‑121 (MQ=255)                                                                                                 tGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                  <  2:1585290/139‑1 (MQ=255)                                                                                                      aaGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTgcgc                                         >  1:1217574/1‑127 (MQ=255)                                                                                                      aaGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTgcgc                                         <  2:1217574/127‑1 (MQ=255)                                                                                                      aaGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATc                             >  1:93771/1‑139 (MQ=255)                                                                                                            tGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGt                       >  1:762047/1‑139 (MQ=255)                                                                                                             gCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                  >  2:637767/1‑127 (MQ=255)                                                                                                             gCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                  <  1:637767/127‑1 (MQ=255)                                                                                                              cTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTaa                     <  2:1412025/139‑1 (MQ=255)                                                                                                                 gagaAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGt                  <  2:139251/139‑1 (MQ=255)                                                                                                                  agaAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTc                 >  1:1597270/1‑139 (MQ=255)                                                                                                                   gaAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGAc                                                                    <  1:1096301/87‑1 (MQ=255)                                                                                                                   gaAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGAc                                                                    >  2:1096301/1‑87 (MQ=255)                                                                                                                   gaAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCa                >  2:1445295/1‑139 (MQ=255)                                                                                                                     aaTACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             >  2:482481/1‑92 (MQ=255)                                                                                                                     aaTACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                             <  1:482481/92‑1 (MQ=255)                                                                                                                      aTACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAAct                                                   <  1:462040/101‑1 (MQ=255)                                                                                                                      aTACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAAct                                                   >  2:462040/1‑101 (MQ=255)                                                                                                                        aCGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                      >  2:667318/1‑64 (MQ=255)                                                                                                                        aCGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                      <  1:667318/64‑1 (MQ=255)                                                                                                                        aCGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                  >  1:1344641/1‑116 (MQ=255)                                                                                                                        aCGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                  <  2:1344641/116‑1 (MQ=255)                                                                                                                           ggCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                          <  1:1168746/57‑1 (MQ=255)                                                                                                                           ggCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                          >  2:1168746/1‑57 (MQ=255)                                                                                                                           ggCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                      >  2:1378907/1‑61 (MQ=255)                                                                                                                           ggCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                      <  1:1378907/61‑1 (MQ=255)                                                                                                                            gCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                                                               <  1:619226/67‑1 (MQ=255)                                                                                                                            gCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                                                               >  2:619226/1‑67 (MQ=255)                                                                                                                                 aTTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGga  <  1:236809/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGA  >  NC_000913/905611‑905877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑