Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,006,491 C→T V180V (GTC→GTT
*370* (TAG→TAA
zapC →
ycbX ←
FtsZ stabilizer
6‑N‑hydroxylaminopurine detoxification oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,006,4910CT96.8% 190.1 / ‑2.2 62V180V (GTC→GTT
*370* (TAG→TAA
zapC
ycbX
FtsZ stabilizer
6‑N‑hydroxylaminopurine detoxification oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (31/29);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCAACTTCCACA  >  NC_000913/1006361‑1006619
                                                                                                                                  |                                                                                                                                
tATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGcc                                                                                                                         <  2:778191/139‑1 (MQ=255)
   gCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCaa                                                                                                                       >  2:1396214/1‑139 (MQ=255)
              caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGt                                                                                                           <  1:1570697/139‑1 (MQ=255)
                 cccTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                                                                                                        <  1:1456228/139‑1 (MQ=255)
                  ccTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                                                                                                        <  1:1396214/139‑1 (MQ=255)
                     gCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTa                                                                                                                                >  2:827462/1‑111 (MQ=255)
                     gCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGt                                                                                                    >  1:534889/1‑139 (MQ=255)
                     gCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGt                                                                                                    >  2:1070623/1‑139 (MQ=255)
                           ttATTGCGGGTCGCGCGATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                                                                                              >  2:661857/1‑139 (MQ=255)
                            tATTGCGGGTCGCGCGATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGc                                                                                             <  1:1624491/139‑1 (MQ=255)
                            tATTGCGGGTCGCGCGATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGc                                                                                             >  2:1455331/1‑139 (MQ=255)
                                     tCGCGCGATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa                                                                                    >  1:592164/1‑139 (MQ=255)
                                     tCGCGCGATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa                                                                                    >  2:1728685/1‑139 (MQ=255)
                                        cgcgATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGcc                                                                                                                         >  2:107345/1‑99 (MQ=255)
                                        cgcgATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGcc                                                                                                                         <  1:107345/99‑1 (MQ=255)
                                        cgcgATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCa                                                                                                             <  2:1428471/111‑1 (MQ=255)
                                        cgcgATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCa                                                                                                             >  1:1428471/1‑111 (MQ=255)
                                        cgcgATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGaa                                                                                 <  2:1266493/139‑1 (MQ=255)
                                          cgATGCAGTTGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATg                                                                               <  1:1455331/139‑1 (MQ=255)
                                                    tggCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCcatca                                                                      <  2:624936/138‑1 (MQ=255)
                                                         aCGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGcc                                                                 <  1:661857/139‑1 (MQ=255)
                                                         aCGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGcc                                                                 <  1:1070623/139‑1 (MQ=255)
                                                          cGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGccc                                                                <  1:878028/139‑1 (MQ=255)
                                                             cATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCa                                                                                                             <  2:1693900/91‑1 (MQ=255)
                                                             cATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCa                                                                                                             >  1:1693900/1‑91 (MQ=255)
                                                                          aaCGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCa                                                >  2:297214/1‑139 (MQ=255)
                                                                            cGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                                                                                                        >  1:457458/1‑81 (MQ=255)
                                                                            cGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCtt                                                                                                        <  2:457458/81‑1 (MQ=255)
                                                                              aCAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTa                                                                                           >  1:399620/1‑92 (MQ=255)
                                                                              aCAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTa                                                                                           <  2:399620/92‑1 (MQ=255)
                                                                               cAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAgcggc                                           >  1:1282559/1‑139 (MQ=255)
                                                                                      aaaCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACgcgc                                                                                                                          <  1:1153041/53‑1 (MQ=255)
                                                                                      aaaCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACgcgc                                                                                                                          >  2:1153041/1‑53 (MQ=255)
                                                                                      aaaCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCa                                                                                                             >  2:733822/1‑66 (MQ=255)
                                                                                      aaaCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCa                                                                                                             <  1:733822/66‑1 (MQ=255)
                                                                                      aaaCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAA‑‑CGCCAACTTAAGTGCAGTCTTCGGTACTCAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCg                                  >  1:1043963/1‑139 (MQ=255)
                                                                                       aaCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAAcgc                                                                                                                            >  2:727873/1‑50 (MQ=255)
                                                                                       aaCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAAcgc                                                                                                                            <  1:727873/50‑1 (MQ=255)
                                                                                            cAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAg                                               <  2:790381/122‑1 (MQ=255)
                                                                                            cAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAg                                               >  1:790381/1‑122 (MQ=255)
                                                                                            cAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCtt                              >  2:1445922/1‑139 (MQ=255)
                                                                                            cAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCtt                              >  1:1116563/1‑139 (MQ=255)
                                                                                              ggTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCt                            <  2:534889/139‑1 (MQ=255)
                                                                                                         aCAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAg                                               <  2:962920/109‑1 (MQ=255)
                                                                                                         aCAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAg                                               >  1:962920/1‑109 (MQ=255)
                                                                                                         aCAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAac                 >  1:381421/1‑139 (MQ=255)
                                                                                                         aCAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAac                 >  1:1428953/1‑139 (MQ=255)
                                                                                                             cTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCa                                                                                                             <  1:1364609/43‑1 (MQ=255)
                                                                                                             cTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCa                                                                                                             >  2:1364609/1‑43 (MQ=255)
                                                                                                             cTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTg                                                                            >  1:709462/1‑76 (MQ=255)
                                                                                                             cTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTg                                                                            <  2:709462/76‑1 (MQ=255)
                                                                                                             cTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCa                                                               >  2:1690367/1‑89 (MQ=255)
                                                                                                             cTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCa                                                               <  1:1690367/89‑1 (MQ=255)
                                                                                                             cTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAgcggc                                           <  1:877694/109‑1 (MQ=255)
                                                                                                             cTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAgcggc                                           >  2:877694/1‑109 (MQ=255)
                                                                                                              ttCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGaa                                                                                 >  2:1313528/1‑70 (MQ=255)
                                                                                                              ttCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGaa                                                                                 <  1:1313528/70‑1 (MQ=255)
                                                                                                                       gAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaa                                                                                     >  1:1553753/1‑57 (MQ=255)
                                                                                                                       gAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaa                                                                                     <  2:1553753/57‑1 (MQ=255)
                                                                                                                        aaCAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGc                                 >  1:1499327/1‑108 (MQ=255)
                                                                                                                        aaCAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGc                                 <  2:1499327/108‑1 (MQ=255)
                                                                                                                        aaCAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCAACTTCcaca  <  1:297214/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                
TATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCAACTTCCACA  >  NC_000913/1006361‑1006619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: