Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,102,221	T→C	E107E (GAA→GAG)	csgE ←	curlin secretion specificity factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,102,221	0	T	C	100.0%	277.8 / NA	77	E107E (GAA→GAG)	csgE	curlin secretion specificity factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/40);  total (37/40)

GAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTT  >  NC_000913/1102087‑1102354                                                                                                                                       |                                                                                                                                      gAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGcctctt                                                                                                                                   <  1:1380285/139‑1 (MQ=255)  aaaTAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCctcttc                                                                                                                                  >  2:1432845/1‑139 (MQ=255)                 gAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCAGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGc                                                                                                                   >  2:1238145/1‑139 (MQ=255)                    ctactGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCaaa                                                                                                                <  1:158822/139‑1 (MQ=255)                       ctGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAgac                                                                                                             >  2:817461/1‑139 (MQ=255)                            tGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAg                                                                                                        >  2:1251632/1‑139 (MQ=255)                              tttGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGtt                                                                                                      <  1:817461/139‑1 (MQ=255)                                       cATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCg                                                                                                >  1:738177/1‑136 (MQ=255)                                       cATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCg                                                                                                <  2:738177/136‑1 (MQ=255)                                        aTTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                            <  1:1238145/139‑1 (MQ=255)                                         ttGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGtc                                                                                           >  1:1472997/1‑139 (MQ=255)                                          tGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGtct                                                                                          <  2:1679321/139‑1 (MQ=255)                                          tGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGtct                                                                                          <  1:944635/139‑1 (MQ=255)                                                      aCAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                            >  2:145181/1‑125 (MQ=255)                                                      aCAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                            <  1:145181/125‑1 (MQ=255)                                                       cAATTTTTTATTTAGAATTCATCATGCGCCAAATCGGCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGaa                                                                             <  1:1716421/139‑1 (MQ=255)                                                                           atcatGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATa                                                         <  1:1076834/139‑1 (MQ=255)                                                                            tcatGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGtt                                                                                                                              >  1:1116006/1‑69 (MQ=255)                                                                            tcatGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGtt                                                                                                                              <  2:1116006/69‑1 (MQ=255)                                                                               tGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAAt                                                                                                                         <  1:1027771/71‑1 (MQ=255)                                                                               tGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAAt                                                                                                                         >  2:1027771/1‑71 (MQ=255)                                                                                gcgcCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGt                                                                   <  2:491763/124‑1 (MQ=255)                                                                                gcgcCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGt                                                                   >  1:491763/1‑124 (MQ=255)                                                                                gcgcCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTc                                                                  <  1:1253763/125‑1 (MQ=255)                                                                                gcgcCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTc                                                                  >  2:1253763/1‑125 (MQ=255)                                                                                gcgcCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTc                                                    >  2:1380503/1‑139 (MQ=255)                                                                                   ccAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGa                                                                                               >  2:1122885/1‑93 (MQ=255)                                                                                   ccAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGa                                                                                               <  1:1122885/93‑1 (MQ=255)                                                                                        tCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGa                                            <  1:42755/139‑1 (MQ=255)                                                                                           cccGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAAt                                                                                                                         >  2:371434/1‑59 (MQ=255)                                                                                           cccGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAAt                                                                                                                         <  1:371434/59‑1 (MQ=255)                                                                                           cccGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGa                                            >  1:359598/1‑136 (MQ=255)                                                                                           cccGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGa                                            <  2:359598/136‑1 (MQ=255)                                                                                             cGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                            <  1:848261/86‑1 (MQ=255)                                                                                             cGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGt                                                                                            >  2:848261/1‑86 (MQ=255)                                                                                              gTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATaaa                                                                        >  1:892072/1‑105 (MQ=255)                                                                                              gTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATaaa                                                                        <  2:892072/105‑1 (MQ=255)                                                                                                      taaCGCCTGGTTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATc                              >  2:822140/1‑139 (MQ=255)                                                                                                       aaCGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATcc                             <  1:1432845/139‑1 (MQ=255)                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAAt                                                                                                                         >  1:1309466/1‑45 (MQ=255)                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAAt                                                                                                                         <  2:1309466/45‑1 (MQ=255)                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGt                                                                   >  1:662634/1‑99 (MQ=255)                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGt                                                                   <  2:662634/99‑1 (MQ=255)                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc                                           >  1:387626/1‑123 (MQ=255)                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc                                           <  2:387626/123‑1 (MQ=255)                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAg                           >  2:1263944/1‑139 (MQ=255)                                                                                                         cGCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAg                           >  1:1535699/1‑139 (MQ=255)                                                                                                          gacTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACg                                                      <  1:1173701/109‑1 (MQ=255)                                                                                                          gCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAc                                                                                 >  1:1532938/1‑84 (MQ=255)                                                                                                          gCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAAc                                                                                 <  2:1532938/84‑1 (MQ=255)                                                                                                          gCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACg                                                      >  2:1173701/1‑111 (MQ=255)                                                                                                          gCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCCAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGa                                            <  1:183727/121‑1 (MQ=255)                                                                                                          gCCTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTGTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGa                                            >  2:183727/1‑121 (MQ=255)                                                                                                           ccTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGtt                                                                                                      <  1:1612538/62‑1 (MQ=255)                                                                                                           ccTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGtt                                                                                                      >  2:1612538/1‑62 (MQ=255)                                                                                                           ccTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCt                         >  1:1588974/1‑139 (MQ=255)                                                                                                            cTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                              <  2:1294245/117‑1 (MQ=255)                                                                                                            cTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                              >  1:1294245/1‑117 (MQ=255)                                                                                                            cTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCg                                         >  1:223894/1‑122 (MQ=255)                                                                                                            cTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCg                                         <  2:223894/122‑1 (MQ=255)                                                                                                            cTGATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCtt                        >  2:1340475/1‑139 (MQ=255)                                                                                                              gATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                      <  2:1588974/139‑1 (MQ=255)                                                                                                              gATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                      <  2:1472997/139‑1 (MQ=255)                                                                                                              gATTTATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                      <  1:1340475/139‑1 (MQ=255)                                                                                                                 ttATCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCa                   <  1:1251632/139‑1 (MQ=255)                                                                                                                   aTCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATc                 <  1:1380503/139‑1 (MQ=255)                                                                                                                    tCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                      >  2:1610705/1‑133 (MQ=255)                                                                                                                    tCTGGCGACGATTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                      <  1:1610705/133‑1 (MQ=255)                                                                                                                         cgacgaTTTAGTGCCTCTTCAGTTTGAATCAGTGCaaa                                                                                                                >  1:1423813/1‑38 (MQ=255)                                                                                                                         cgacgaTTTAGTGCCTCTTCAGTTTGAATCAGTGCaaa                                                                                                                <  2:1423813/38‑1 (MQ=255)                                                                                                                         cgacgaTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                              >  2:1624764/1‑104 (MQ=255)                                                                                                                         cgacgaTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGAtt                                              <  1:1624764/104‑1 (MQ=255)                                                                                                                              aTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTg         <  1:1605694/136‑1 (MQ=255)                                                                                                                              aTTTAGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTg         >  2:1605694/1‑136 (MQ=255)                                                                                                                                  aGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc                                           >  1:1284520/1‑98 (MQ=255)                                                                                                                                  aGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGAc                                           <  2:1284520/98‑1 (MQ=255)                                                                                                                                  aGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                      >  2:1174277/1‑119 (MQ=255)                                                                                                                                  aGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTcc                      <  1:1174277/119‑1 (MQ=255)                                                                                                                                  aGTGCCTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCttt  >  2:835169/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                      GAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTT  >  NC_000913/1102087‑1102354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑