Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,208,517	C→T	P129L (CCT→CTT)  *201* (TAG→TAA)	tfaP → tfaE ←	e14 prophage, uncharacterized protein e14 prophage, putative tail fiber assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,208,517	0	C	T	100.0%	194.7 / NA	65	P129L (CCT→CTT)  *201* (TAG→TAA)	tfaP tfaE	e14 prophage, uncharacterized protein e14 prophage, putative tail fiber assembly protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/28);  total (37/28)

TAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGG  >  NC_000913/1208383‑1208644                                                                                                                                       |                                                                                                                                tAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACg                                                                                                                             >  1:98396/1‑139 (MQ=255)      gACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTcc                                                                                                                        >  2:832042/1‑139 (MQ=255)           tGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTAGGCCTCCGGCAg                                                                                                                   >  1:1133319/1‑139 (MQ=255)                gggTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                       <  2:1625018/130‑1 (MQ=255)                gggTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCg                                                                                                                       >  1:1625018/1‑130 (MQ=255)                                 gAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                            <  2:1133319/139‑1 (MQ=255)                                  aaGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcgc                                                                                           <  1:1502704/139‑1 (MQ=255)                                     aCTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGt                                                                                        >  2:921083/1‑139 (MQ=255)                                          aagaagATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTgg                                                                                   >  1:1497886/1‑139 (MQ=255)                                               gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATc                                                                                                 >  1:1064543/1‑120 (MQ=255)                                               gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATc                                                                                                 <  2:1064543/120‑1 (MQ=255)                                               gaTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCt                                                                              <  2:98396/139‑1 (MQ=255)                                                aTGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTg                                                                             >  2:1318296/1‑139 (MQ=255)                                                         aaCAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                            >  2:22605/1‑115 (MQ=255)                                                         aaCAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                            <  1:22605/115‑1 (MQ=255)                                                                     tgtgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGt                                                        >  1:828180/1‑139 (MQ=255)                                                                      gtgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATg                                                                                                      >  2:1147371/1‑92 (MQ=255)                                                                      gtgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATg                                                                                                      <  1:1147371/92‑1 (MQ=255)                                                                       tgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         >  1:394554/1‑136 (MQ=255)                                                                       tgGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         <  2:394554/136‑1 (MQ=255)                                                                        ggCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAAt                                                     >  1:1190948/1‑139 (MQ=255)                                                                                 aTCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAg                                            >  1:1520818/1‑139 (MQ=255)                                                                                       aaGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         <  1:229679/120‑1 (MQ=255)                                                                                       aaGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         >  2:229679/1‑120 (MQ=255)                                                                                            cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGtt                                        <  1:816101/132‑1 (MQ=255)                                                                                            cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGtt                                        >  2:816101/1‑132 (MQ=255)                                                                                            cTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTg                                 >  2:684488/1‑139 (MQ=255)                                                                                             tGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                              <  2:1318394/109‑1 (MQ=255)                                                                                             tGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                              >  1:1318394/1‑109 (MQ=255)                                                                                              ggAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGgt                               >  2:309696/1‑139 (MQ=255)                                                                                                   tGGTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGttt                          <  2:1520818/139‑1 (MQ=255)                                                                                                     gTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGt                                                                                        >  2:1373422/1‑75 (MQ=14)                                                                                                     gTTGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGt                                                                                        <  1:1373422/75‑1 (MQ=17)                                                                                                      ttGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         <  1:1639423/105‑1 (MQ=255)                                                                                                      ttGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         >  2:1639423/1‑105 (MQ=255)                                                                                                      ttGATACATCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTctg                       >  2:849700/1‑139 (MQ=255)                                                                                                             aTCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                <  2:1497886/139‑1 (MQ=255)                                                                                                             aTCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                <  1:684488/139‑1 (MQ=255)                                                                                                              tCCAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTc               >  2:1570534/1‑139 (MQ=255)                                                                                                               ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGttagg                                                                                                              <  1:1270033/44‑4 (MQ=14)                                                                                                               ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGttagg                                                                                                              >  2:1270033/1‑41 (MQ=11)                                                                                                               ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         <  1:1025049/96‑1 (MQ=255)                                                                                                               ccAGTGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCATCACCGCGTCAATg                                                         >  2:1025049/1‑96 (MQ=11)                                                                                                                   tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCGGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGttt          >  2:471982/1‑139 (MQ=255)                                                                                                                   tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGt                                                                                  >  2:621171/1‑67 (MQ=11)                                                                                                                   tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGt                                                                                  <  1:621171/67‑1 (MQ=11)                                                                                                                   tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         <  1:153653/92‑1 (MQ=255)                                                                                                                   tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATg                                                         >  2:153653/1‑92 (MQ=255)                                                                                                                   tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTctgcc                     >  1:1105718/1‑128 (MQ=255)                                                                                                                   tGCGCCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTctgcc                     <  2:1105718/128‑1 (MQ=255)                                                                                                                     cgcCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGtt           >  1:1504181/1‑136 (MQ=255)                                                                                                                     cgcCAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGtt           <  2:1504181/136‑1 (MQ=255)                                                                                                                       ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                   <  2:1048035/78‑1 (MQ=11)                                                                                                                       ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCAc                                                                   >  1:1048035/1‑78 (MQ=11)                                                                                                                       ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                <  1:831036/129‑1 (MQ=255)                                                                                                                       ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGt                >  2:831036/1‑129 (MQ=255)                                                                                                                       ccAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGt      >  1:737497/1‑139 (MQ=255)                                                                                                                        cAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                            <  2:1281454/52‑1 (MQ=11)                                                                                                                        cAGATATTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGgcg                                                                                            >  1:1281454/1‑52 (MQ=11)                                                                                                                           atatTGAATGGCTTACGCGTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCgg  >  2:960755/1‑139 (MQ=255)                                                                                                                           atatTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCgg  <  1:921083/139‑1 (MQ=255)                                                                                                                           atatTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCgg  <  1:832042/139‑1 (MQ=255)                                                                                                                           atatTGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGCTTCTGCCTGCGTCAGTTTACGTCCgg  >  2:1100937/1‑139 (MQ=255)                                                                                                                               tGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                              <  2:750863/75‑1 (MQ=11)                                                                                                                               tGAATGGCTTACGCCTCCGGCAGTT‑AGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGt                                                              >  1:750863/1‑75 (MQ=11)                                                                                                                                       |                                                                                                                                TAGCAGACAGTGGCAGGGTAAGGCTGCATTGGGAAGACTTAAAGAAGATGAGCTGAAACAATATAATTTGTGGCTGGATTATCTGGAAGCACTGGAACTGGTTGATACATCCAGTGCGCCAGATATTGAATGGCCTACGCCTCCGGCAGTTCAGGCCAGATGACATCCGGCGCGGTGCTGGTATCTGTTGCCGTCACCGCGTCAATGTAATCCAGCACAGCGTTAAGTCTGGTTGTTTCTGCCTGCGTCAGTTTACGTCCGG  >  NC_000913/1208383‑1208644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑