| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 117,074 | A→G | L9P (CTG→CCG) | hofB ← | T2SE secretion family protein, P‑loop ATPase superfamily protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 117,074 | 0 | A | G | 90.5% | 190.8 / 6.4 | 64 | L9P (CTG→CCG) | hofB | T2SE secretion family protein, P‑loop ATPase superfamily protein |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (28/29); total (32/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
CAATACGTTTGGTGGTAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGT > NC_000913/116952‑117206 | cAATACGTTTGGTGGTAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTgg > 2:1254398/1‑139 (MQ=255) gTTTGGTGGTAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATAtt > 2:278932/1‑139 (MQ=255) ttGGTGGTAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCa > 2:337733/1‑139 (MQ=255) ttGGTGGTAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCa > 2:1722281/1‑139 (MQ=255) gtggtAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTg < 1:611847/139‑1 (MQ=255) aGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCt < 2:505143/139‑1 (MQ=255) atgcGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGc < 2:1367896/139‑1 (MQ=255) tCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGtcat < 1:93784/139‑1 (MQ=255) tCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGtcat < 1:1254398/139‑1 (MQ=255) ccAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGtcatc > 1:1417135/1‑139 (MQ=255) ccAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGtcatc > 2:364275/1‑139 (MQ=255) cAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGtcatca < 2:1417135/139‑1 (MQ=255) aGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGc > 1:1517441/1‑116 (MQ=255) aGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCACTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGc < 2:1517441/116‑1 (MQ=255) cTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCgg > 2:178013/1‑139 (MQ=255) cATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGaa > 1:604880/1‑139 (MQ=255) ggTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGt < 2:199259/80‑1 (MQ=255) ggTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGt > 1:199259/1‑80 (MQ=255) tGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCg < 1:1127066/139‑1 (MQ=255) cATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCt > 2:1497445/1‑81 (MQ=255) cATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCt < 1:1497445/81‑1 (MQ=255) aTCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTc > 1:1415482/1‑101 (MQ=255) aTCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTc < 2:1415482/101‑1 (MQ=255) aTCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAg > 2:590494/1‑139 (MQ=255) aTCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAg < 1:337733/139‑1 (MQ=255) aCTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGa < 1:525352/81‑1 (MQ=255) aCTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGa > 2:525352/1‑81 (MQ=255) aCTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACa > 1:398037/1‑126 (MQ=255) aCTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACa < 2:398037/126‑1 (MQ=255) aCTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCtt > 2:863195/1‑139 (MQ=255) tACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGt > 2:382587/1‑69 (MQ=255) tACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGt < 1:382587/69‑1 (MQ=255) aCCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGcc > 1:1403188/1‑90 (MQ=255) aCCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGcc < 2:1403188/90‑1 (MQ=255) cAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGtcatca > 2:1057876/1‑107 (MQ=255) cAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGACCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGtcatca < 1:1057876/107‑1 (MQ=255) aaCCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGc < 1:780793/93‑1 (MQ=255) aaCCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGc > 2:780793/1‑93 (MQ=255) accTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCAc > 1:1102182/1‑139 (MQ=255) ccTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACt < 2:974012/139‑1 (MQ=255) ccTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACt < 1:958861/139‑1 (MQ=255) ccTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACt < 1:178013/139‑1 (MQ=255) ctctTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACAt > 2:599335/1‑107 (MQ=255) ctctTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACAt < 1:599335/107‑1 (MQ=255) ctctTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACtt > 2:1138779/1‑139 (MQ=255) ctctTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACCGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACtt > 2:1278510/1‑139 (MQ=255) ctTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATg > 2:1347775/1‑126 (MQ=255) ctTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATg < 1:1347775/126‑1 (MQ=255) tCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGaa > 2:201921/1‑139 (MQ=255) tCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGaa < 1:1722281/139‑1 (MQ=255) cGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCt < 2:1326639/51‑1 (MQ=255) cGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCt > 1:1326639/1‑51 (MQ=255) tGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGcc < 1:794884/65‑1 (MQ=255) tGGCATCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGcc > 2:794884/1‑65 (MQ=255) aTCCAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAg < 1:470784/91‑1 (MQ=255) aTCCAGCAAGACTCCATGAAAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAg > 2:470784/1‑91 (MQ=255) cAGCAAGACTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTg < 1:1530664/139‑1 (MQ=255) aaGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGcgcg < 1:863195/139‑1 (MQ=255) cTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGc > 1:800261/1‑49 (MQ=255) cTCCATGATAACGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGc < 2:800261/49‑1 (MQ=255) cGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTg > 1:1296086/1‑37 (MQ=255) cGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTg < 2:1296086/37‑1 (MQ=255) cGCAGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGc > 1:629989/1‑139 (MQ=255) aGACACGGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGt < 1:522398/139‑1 (MQ=255) | CAATACGTTTGGTGGTAGCGAAATGCAATGCGTCCAGTAGCTCATGCGAAGGTGCATCGACTACCGCAACATGAACCACCTCTTCGCTGGCATCCAGCAAGACTCCATGATAACGCAGACACAGGGCAGTGAGCTGTGGAATATTCATTGCCGCTCCTTAGTTGGCGTCATCAAAGCGGAAGACATCTTCGCAGGCTTGCTGCAATGCGCTGTCACTTTGAATATTGCAGTTGCGCGTCCAGCCGGTGACGCCGT > NC_000913/116952‑117206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |